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Citations for New England Biolabs :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... using Phusion High-Fidelity DNA Polymerase (New England Biolabs), as described previously [31] ...
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bioRxiv - Microbiology 2024Quote: ... as well as Q5 High-Fidelity DNA Polymerase (New England Biolabs Inc; 0.4 unit) and PCR buffer (5 ×) ...
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bioRxiv - Microbiology 2024Quote: All animal use was approved under ACUP EB17-033-002 (Cidara/Explora Biolabs, San Diego).
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bioRxiv - Microbiology 2024Quote: ... restriction enzymes and T7 ligase (NEB Biolabs, USA) were used according to manual ...
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bioRxiv - Immunology 2024Quote: ... The construct was transformed in T7 Express E coli (NEB) and grown to O.D ...
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bioRxiv - Immunology 2024Quote: ... 0.5μL 20x WarmStart RT Enzyme Mix (NEB), 0.5μL of target gene specific forward and reverse primers and probe mix as previously described8 ...
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bioRxiv - Microbiology 2024Quote: ... followed by excision and ligation for 2 hours at room temperature using the Instant Sticky-end Ligase Master Mix (NEB) in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Thawed RNA specimens were converted to cDNA using the Luna RT mix (NEB). Arctic multiplex PCR primer pools (IDT ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5-α competent cells (NEB) following the company’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5-α competent cells (C2987H, NEB—New England BioLabs Inc., Ipswich, USA) as a host.
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bioRxiv - Microbiology 2024Quote: ... coli DH5-α competent cells (C2987H, NEB—New England BioLabs Inc., Ipswich, USA) as a host.
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bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: All constructs in this study were generated using Gibson Assembly cloning (New England Biolabs). The targeting vector containing heterologous enhancers (e.g. ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed with Luna Universal qPCR Master Mix (Biolabs) using the Step-One Real-time PCR system (Life Technologies) ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s guidelines ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... The USER enzyme (NEB, USA) was introduced to the cDNA of the selected size and adaptor ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of total RNA was used for mRNA enrichment by using the NEBNext mRNA polyA Isolation Module(NEB, #E7490L) and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNA was treated with PNK4 before being used for cDNA synthesis using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (NEB). Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB) for sequencing.
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bioRxiv - Cell Biology 2024Quote: ... primers with homology overhangs for pDT7 (L4440) (Timmons and Fire, 1998, Timmons et al., 2001) were designed via NEBuilder Assembly Tool (New England Biolabs Inc., Ipswich, USA) (see Appendix Table S13) ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation of DNA regions in close physical proximity was performed using 1,000 U of T4 DNA ligase (NEB) for overnight ...
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bioRxiv - Cell Biology 2024Quote: ... DNA amount was measured by Qubit and NEBNext Ultra II (NEB) used for library prep ...
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bioRxiv - Cell Biology 2024Quote: ... amplified by PCR (15 cycles, Phusion, NEB) and cloned into the XhoI and EcoRI sites of pSC2 as previously described(38 ...
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bioRxiv - Cell Biology 2024Quote: ... and Hind III (NEB) at 37°C for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was isolated from total RNA using NEBNext Poly(A) mRNA magnetic isolation module (NEB cat# E7490. Primers (below) were designed to recognize the 5’ half of the mRNA ...
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bioRxiv - Cell Biology 2024Quote: ... and was placed in a shuttle vector encoding the rat insulin promoter and rabbit beta-globin intron (a gift from Matt Merrins) (43) between EcoRI and XhoI with a T2A and BFP cloned by HiFi (NEB). Sequence was validated by Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... cAMP-22p was cloned into pEN-RIP1-BGH-pA vector with T2a-h2b-mCherry between the EcoRI and XhoI by HiFi assembly (NEB, E2621). The correct sequence was validated by Sanger sequencing and virus was produced as above ...
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed with glycoprotein denaturing buffer (B1704S, NE BioLabs). Each sample was sonicated ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were then treated with 500 U of peptide-N-glycosidase F (PNGase F; P0704L, NE BioLabs) in a reaction solution containing 50 mM sodium phosphate (pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... and treated with proteinase K (P8107, NE BioLabs) at different concentrations for 10 min at room temperature (R.T.) ...
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bioRxiv - Cell Biology 2024Quote: ... in a reaction solution containing 50 mM sodium phosphate (pH 7.5) and 1% NP-40 (B2704S, NE BioLabs) for 1 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared from rRNA depleted samples using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on the Novaseq 6000 S4 (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... PCR enrichment of adaptor-ligated DNA was conducted with five cycles using NEBNext Multiplex Oligos for Illumina (NEB, USA) for paired-end barcoding ...
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bioRxiv - Immunology 2024Quote: ... Using NEBuilder HiFi DNA assembly (NEB), the E ...
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bioRxiv - Cell Biology 2024Quote: H in the reaction buffer containing GlycoBuffer 3 (B1720S, NE BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... The miRNA:mRNA chimeric ligation step was done at room temperature for 1hr with T4 RNA ligase (NEB). The IP samples were then treated with alkaline phosphatase (FastAP ...
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bioRxiv - Cell Biology 2024Quote: ... by Gibson assembly (NEB). A DNA mix containing pCZ986 (50 ng/µL) ...
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bioRxiv - Developmental Biology 2024Quote: ... The generated cDNA was used as the template for qPCR analysis by utilizing Universal qPCR Master Mix (BioLabs). At least three independent experiments were performed to indicate the change in gene transcription ...
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bioRxiv - Cell Biology 2024Quote: ... Double digested PCR product and plasmid were further subjected to Ligation using T4 DNA ligase (NEB) at 16°C for 12-14 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product and Plasmid were subjected to restriction digestion by using Xho1 (NEB) and Hind III (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were sent to the Penn State Genomics Core Facility for library preparation using NEBNext® UltraTM II DNA Library Prep kits for Illumina® with NEBNext® Multiplex Oligos (New England Biolabs, NEB: Ipswich, MA). Each library was prepared from 500ng DNA ...
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bioRxiv - Cell Biology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554), the DpnI (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 375 U of MboI (NEB, R0147) was used to digest ...
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bioRxiv - Genomics 2024Quote: ... the DNA was ligated by T4 DNA ligase (NEB # M0202) at 4 °C overnight ...
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bioRxiv - Genomics 2024Quote: ... 360 μl 10x T4 DNA ligase buffer (NEB, B0202), 15 μl of 400 U/μl T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 10 µL of tagmented DNA was PCR amplified for 14 cycles in a 50 µL reaction volume using NEBNext High-Fidelity 2X PCR Master Mix (NEB # M0541S) and Nextera primers ...
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bioRxiv - Genomics 2024Quote: ... 75 μl of NEBuffer™ DpnII (NEB, B0543S) and 600U of DpnII (R0543M ...