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Citations for New England Biolabs :
701 - 750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... using the NEBuilder HiFi DNA Assembly kit (New England Biolabs); by strictly following the protocols recommended by the manufacturers ...
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bioRxiv - Biophysics 2024Quote: ... Gi was incubated in 0.02% DDM/CHS on ice for 30 minutes before addition of 0.5 µl apyrase (NEB) and ScFV at a 1:1 ratio (the final concentrations of Gi and ScFV were both ∼90 µM ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Biophysics 2024Quote: ... Then the suspension was incubated at room temperature for 1.5 h after adding apyrase (25 mU/mL; New England Biolabs). The supernatant was removed by ultracentrifugation at 40,000 rpm for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... Escherichia coli BL21 (DE3) competent cells (New England Biolabs) were transformed with a pET28a vector containing the N-terminal six-histidine tagged human TG2 ...
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bioRxiv - Biophysics 2024Quote: ... The resulting construct was expressed in Escherichia coli BL21(DE3) cells (New England Biolabs, C2527H) at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio ...
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bioRxiv - Cancer Biology 2024Quote: ... These gene fragments were cloned into pcDNA3.1-PsChR2-eYFP backbone vector using the HiFi Assembly Kit (New England Biolabs, lpswich, MA).
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bioRxiv - Cancer Biology 2024Quote: ... linearized with BsmBI (NEB, #R0739S) and a PCR based insert produced by Phusion DNA Polymerase (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... by In Fusion cloning into pDual_dsCas9_Venus digested with BamHI-HF (NEB, #R3136). Dual-sgRNAs against chromosome 1 non-coding locus ...
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bioRxiv - Cancer Biology 2024Quote: ... and a PCR based insert produced by Phusion DNA Polymerase (NEB, #M0530L) containing a second sgRNA cloning site (SbfI) ...
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bioRxiv - Cancer Biology 2024Quote: ... EcoRI (NEB, USA) and BsmI (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... or BsmBI (NEB, #R0739S). All plasmids were verified by sequencing and deposited with Addgene (#214678–214692) ...
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bioRxiv - Cancer Biology 2024Quote: ... and introduced by In Fusion cloning into pDual_dsCas9_Venus/BFP/Puro/Blast linearized with SbfI (NEB, #R0642S) or BsmBI (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.025 U/ml Taq polymerase (NEB, #M0267) in a final reaction volume of 15 µl and published thermal cycling parameters [17] ...
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bioRxiv - Cancer Biology 2024Quote: ... Double-stranded cDNA was then synthesized by reverse transcription and underwent end-repair and ligation using New England Biolabs (NEB) adapters ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR products then underwent end-repair and A-tail addition followed by New England Biolabs (NEB) adapter ligation ...
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bioRxiv - Cell Biology 2024Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). Proteins were transfected into Rosetta2 (DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... was fused to the cDNA sequence of mScarlet-I by PCR and subcloned into pMNATZA1 using Gibson assembly with NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA). The spPKA-KTR1.0 is stably expressed under the Padh1 promoter from the Z locus ...
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bioRxiv - Cell Biology 2024Quote: ... Monarch® DNA Gel Extraction Kits (NEB) were used per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was amplified using the Luna Universal qPCR Master Mix (New England Biolabs M3003E) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA templates were amplified using Phusion polymerase (New England Biolabs) with index- sequenced primers.
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bioRxiv - Cell Biology 2024Quote: ... The residual RNAs were reverse transcribed using ProtoScript II (New England Biolabs) and circularized with circLigase2 (Epicentre) ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA fragment underwent restriction enzyme digest (New England Biolabs) to generate overhangs and was subcloned into the pCCL vector ...
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bioRxiv - Cell Biology 2024Quote: ... and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs). DNA sequences and resultant transformants are listed in Table S6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... we used NheI-HF (New England Biolabs, cat. R3131L) to cut both the AR-DBD_ERT2 construct and the purified fragments ...
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bioRxiv - Cancer Biology 2024Quote: ... and AatII (New England Biolabs, cat. R0117L) to cleave both the MACC constructs and the backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... The process involved using SalI-HF (New England Biolabs, cat. R3138S) and AatII (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs, cat. M0371S) after AR-DBD_ERT2 construct NheI fragmentation to dephosphorylate ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified ChIP DNA was used to prepare Illumina multiplexed sequencing libraries using the NEBNext Ultra II DNA Library Prep kit and the NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped using the Vaccinia capping system (NEB M2080S) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and two BsmBI Type IIS restriction enzyme sites was cloned into the 3’ end of the bovine U6 promoter using Gibson Assembly (NEBuilder HiFi, NEB) (see Supplementary Figure 1a) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µl of thermolabile proteinase K (New England Biolabs, P811S) added to Mitochondria-bound beads resuspended in 30 µl of KPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole exome library construction was generated using the NEB Next® Ultra RNA Library Prep Kit for Illumina (E7530L, NEB, USA) after DNA shearing ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... the sgRNA regions were amplified from the genomic DNA by PCR using the NEBNext Q5 HotStart HiFi polymerase (NEB, M0543) and specific primers ...
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bioRxiv - Cancer Biology 2024Quote: ... the NEB Next® Ultra RNA Library Prep Kit for Illumina (E7530L, NEB, USA) was used ...
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bioRxiv - Cell Biology 2024Quote: ... and Taq polymerase (New England Biolabs). Primers (Supplemental Table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To ensure simultaneous amplification of both the large unedited ( 12.6 kb) and edited allele (646 bp) we used the LongAmp Taq DNA Polymerase (NEB) which is specially formulated to amplify large products ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.1μL HaeIII (NEB – R0108S), 1 μL patient sample gDNA with a concentration range of 10 – 66 ng/μL and 6.7 μL nuclease free water to make a 20 μL total reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped using the Vaccinia Capping System (BioLabs) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification of the BC-sgRNA1-sgRNA2 region from 32 μg of bulk lung genomic DNA using Q5 Ultra II High-Fidelity 2× Master Mix (New England Biolabs, M0494X). Unique dual-indexed primers were used to amplify each sample followed by purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-seq libraries were prepared using the NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) as previously described76 ...
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bioRxiv - Cancer Biology 2024Quote: ... 28 novel RNF43 RING domain variants were generated using Q5 mutagenesis (New England Biolabs). All ZNRF3 and RNF43 expression plasmids were full-length sequence verified ...
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bioRxiv - Cancer Biology 2024Quote: ... The first one made use of Q5 mutagenesis following the manufacturer’s instructions (New England Biolabs), but due to the high GC-content of exon1 specific for the ZNRF3 long isoform ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Forward and reverse primers (100 µM) were phosphorylated and annealed using T4 Polynucleotide Kinase (PNK; New England Biolabs M0201S) and 10x T4 Ligation Buffer (Thermo B69) ...
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bioRxiv - Cancer Biology 2024Quote: ... after which the cells were washed twice with DPBS and total RNA was extracted using the Monarch® Total RNA Miniprep Kit (NEB, Ipswich, MA). Total RNA was stored at −80°C until sequencing ...