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Citations for New England Biolabs :
701 - 750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and PE 2.0 (5′-CAA GCA GAA GAC GGC ATA CGA GAT CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC* T-3′) for 15 cycles using Phusion polymerase (NEB M0530S). The library was purified by electrophoresis on a 1.2% agarose gel to get rid of adapter dimers ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was A-tailed with Fermentas Klenow 3′ to 5′ exonuclease (Cat# EP0421) and ligated with adaptor oligonucleotides (NEB NEXT adaptor oligos) using Mighty Mix Ligase (Cat# TAK6023) ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (New England BioLabs, #E7645S) per manufacturer’s instructions with minor modifications ...
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... IDT synthesized oligonucleotides were annealed and phosphorylated using PNK (NEB#C3019) at 37° C for 30min ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and subsequently transformed into NEB10beta (NEB#C3019). Purified DNA from propagated colonies were confirmed for gRNA insertion via Sanger sequencing (Genewiz) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... in an all-in-one T7 ligase (NEB#M0318S) based Golden-Gate assembly (37° C for 5min ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µl Q5 DNA Polymerase (NEB). Amplification was done with an initial denaturation at 95 °C for 30 sec followed by 40 cycles at 95 °C for 10 sec ...
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bioRxiv - Physiology 2024Quote: ... and Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA). Targeted genes related to gluconeogenesis and glucose transporter were ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng of total RNA from each sample was used for preparing the libraries with the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs, #E7760), using the rRNA depletion module ...
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bioRxiv - Physiology 2024Quote: ... followed by RNA purification using a Monarch RNA Cleanup Kit following the manufacturer’s protocol (New England Biolabs, Whitby, ON, Canada). One-day old male and female adult mosquitoes were briefly anesthetized using CO2 and injected in the thorax with 1 μg of AedaeItp ...
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bioRxiv - Physiology 2024Quote: ... Libraries were constructed from polyA-selected RNA using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) and sequenced on Illumina HiSeq2500 instrument ...
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bioRxiv - Physiology 2024Quote: ... 1mM dNTPs (N0477L, NEB), RNase inhibitor (30281 ...
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bioRxiv - Physiology 2024Quote: ... The flow cell was washed with nuclease-free water three times and incubated with DraI (R0129, NEB) and CIAP (M0525 ...
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bioRxiv - Physiology 2024Quote: Tissue sections were treated with exonuclease I (#M2903, NEB) cocktail the next day in 37 °C for 45 min to remove unused single-stranded DNA probes ...
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bioRxiv - Physiology 2024Quote: ... Klenow Fragment (M0212, NEB), and nuclease-free water ...
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bioRxiv - Physiology 2024Quote: ... 1mM dNTPs (N0477, NEB), Klenow Fragment (M0212 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The construct pcDNA4TO-TwinStrep (TS)-SNAP-β2AR was generated by amplification of the SNAP and β2AR sequences of the pSNAPf-ADRB2 plasmid (NEB) and inserted into pcDNA4TO-TS using Gibson assembly (Heydenreich et al. ...
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bioRxiv - Bioengineering 2024Quote: ... coli Shuffle T7 strain (NEB) was transformed with pET17b OVA-ZE ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... plasmids purified as described above harboring the region of interest were digested by BsmI (NEB, Ipswich MA). The reaction mixture was prepared in a nuclease-free microcentrifuge tube ...
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bioRxiv - Biophysics 2024Quote: ... coli BL21(DE3)T1R (New England Biolabs) was used to express full-length NadA variant 3 following transformation with pET21-NadA ...
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bioRxiv - Developmental Biology 2024Quote: ... and 6 U SalI (New England Biolabs, Cat. No. R0138S)) were incubated with the following condition ...
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bioRxiv - Developmental Biology 2024Quote: ... Reagents (400ng of pT7-gRNA, 0.25 µM annealed oligo, 1x NEBuffer 3.1 (New England Biolabs, Cat. No. B7203), 1x T4 DNA ligase buffer (Promega Co. ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 U BglII (New England Biolabs, Cat. No. R0144S) and 6 U SalI (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... cut with 1 unit of BpiI (NEB) at 37 °C for 1 hour ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Évry-Courcouronnes, France). qRT-PCR was performed in quadruplets on a CFX384 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Plant Biology 2024Quote: ... The anti-pTEpY antibody purchased from Cell Signaling (NEB 4370S) was used for detecting phosphorylated MAPK3 and MAPK6 following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 0.4 μL Buffer Tag DNA polymerase (5 U/μL, NEB, Cat. No. B9004S), 1.6 μL dNTP (TAKARA ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... DNase I treatment (NEB) was employed to eliminate any residual genomic DNA contamination ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized using M-MuLV Reverse Transcriptase (NEB) enzyme ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Enlgand Biolabs, #E7760S/L).
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bioRxiv - Biochemistry 2024Quote: ... The purified PCR product was digested by KpnI and XhoI (NEB, underlined in primer sequences) and repurified ...
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bioRxiv - Biochemistry 2024Quote: ... The SUGCT insert and pcDNA5/FRT vector were ligated by using the Quick Ligation Kit (NEB) and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... reverse 5’-GTGGCC CTCGAG TCA GTG AGT TTC ATG TTG G-3’ and then purified using the Monarch PCR plus DNA purification kit (NEB). The purified PCR product was digested by KpnI and XhoI (NEB ...
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bioRxiv - Physiology 2024Quote: ... The flow cell was then treated with exonuclease I (M2903, NEB) cocktail in 37 °C for 45 min to remove non-specific single-stranded DNA ...
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bioRxiv - Physiology 2024Quote: ... The array was loaded with secondary strand synthesis mixture (1X NEBuffer-2 (NEB), 10 uM TruSeq Read2-conjugated Random Primer (IDT) ...
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bioRxiv - Physiology 2024Quote: ... and CIAP (M0525, NEB) enzyme mixture in 37 °C overnight to expose oligo-dT end ...
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bioRxiv - Physiology 2024Quote: ... 16 U/mL Proteinase K (P8107S, NEB)) at 37 °C for 40 min ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from 30-35 sets of MTs isolated from 5-7-day old female flies in five independent biological replicates using the Monarch Total RNA Miniprep kit (New England BioLabs, Whitby, ON, Canada). Total RNA was quantified using a UV spectrophotometer (Synergy 2 microplate reader ...
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bioRxiv - Biochemistry 2024Quote: ... was used for high-fidelity PCR and the 2X HiFi Gibson Assembly Mix (NEB) was used for amplicon assembly ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Primer sequences and plasmid construct information is listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2024Quote: ... the 2X Q5 Master Mix (NEB) was used for high-fidelity PCR and the 2X HiFi Gibson Assembly Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Acad11 genotypes were assessed using a three-primer PCR strategy with the 5X Multiplex PCR Mix (NEB) to allow for the simultaneous detection of both wild-type and mutant alleles ...
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bioRxiv - Bioengineering 2024Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Bioengineering 2024Quote: ... inserts with homology overhangs for cloning were ordered from IDT and cloned into the pPB-Zeocin backbone using HiFi DNA Assembly Master Mix (NEB). All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Bioengineering 2024Quote: ... coli Protein Synthesis System (NEB) [48] at 2 x the reaction scale ...
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bioRxiv - Plant Biology 2024Quote: ... or ligated using T4 ligase (Thermo Fisher Scientific/New England Biolabs) in corresponding clones.