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Citations for New England Biolabs :
5401 - 5450 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The pooled PCR products were incubated at a 10:1 ratio with DpnI enzyme (New England Biolabs) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were resuspended in 25 μl cDNA amplification mix (1X Phusion HF PCR Master Mix (NEB, #M0531L) and 0.5uM P5 and P7 short primer mix (see Supplementary Table 2) ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed using the Q5® Hot Start High-Fidelity 2X Master Mix (NEB, M0494) following the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2022Quote: Selected Aß42 variants for individual testing were obtained by PCR linearisation (Q5 high-fidelity DNA polymerase, NEB) with mutagenic primers (primers MS_38-47 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR reaction to simultaneously amplify all eight gene segments was performed using Phusion polymerase (NEB #M0530L) as follows ...
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bioRxiv - Microbiology 2022Quote: ... and mucI PCR products were ligated into pBGE using the Gibson Assembly Cloning Kit (New England Biolabs) to generate pmucG-C ...
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bioRxiv - Microbiology 2022Quote: ... Subsequent on-bead PCR indexing-amplification of tagmented DNA was performed using 2x Phusion Master Mix (NEB) and custom-ordered indexing primers (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR fragments were gel purified and equal amounts of DNA were subject to DrdI (New England Biolabs) enzyme digest following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These fragments were PCR-amplified by Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs), visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) ...
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bioRxiv - Microbiology 2022Quote: 50 μl PCR reactions were performed using Q5 Hot start High-Fidelity DNA polymerase (New England Biolabs), PJF119EH-cusF (pAV54 ...
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bioRxiv - Neuroscience 2020Quote: ... target site flanking sequences of approximately 1kb size were amplified by PCR (Q5 polymerase, New England Biolabs) from genomic DNA of nos-Cas9[attP2] fly line used for embryo injection ...
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bioRxiv - Plant Biology 2021Quote: ... DNA fragments were PCR-amplified for 19 cycles using the NEB 2X Taq MasterMix (NEB, MA, USA) and 35 ng of starting DNA in an Eppendorf Mastercycler ep gradient S (Eppendorf ...
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bioRxiv - Neuroscience 2020Quote: ... PCR(20 total cycles) was then carried out using the Q5 Ultra II Q5 Master Mix(NEB) to amplify final libraries using universal Illumina primers ...
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bioRxiv - Neuroscience 2020Quote: ... DNA from 2 biological replicates was purified with Monarch PCR & DNA Cleanup Kit (Cat. no. T1030, NEB). Library preparation ...
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bioRxiv - Microbiology 2020Quote: ... PCR enrichment of adaptor-ligated DNA was performed using NEBNext Multiplex Oligos for Illumina (New England Biolabs) to produce Illumina-ready libraries ...
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bioRxiv - Microbiology 2021Quote: ... and the PCR fragment of the plasmid were assembled together using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
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bioRxiv - Bioengineering 2021Quote: A standard PCR process was performed using Q5 high-fidelity DNA polymerase from NEB (cat. no. M0491S). All PCR primers were purchased from IDT (Supplementary Table S1) ...
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bioRxiv - Biophysics 2021Quote: ... Two of them correspond to two PCR-fabricated DNAs (C1 and C2) derived from lambda DNA (NEB) as template and including suitable restriction sites in the designed primers (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... PCR to amplify constructs for cloning and sequencing were performed using Q5 High-Fidelity DNA polymerase (NEB), OneTaq DNA polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: PCR reactions were done with the Phusion high fidelity DNA polymerase (New England Biolabs Canada, Whitby Ontario) using standard protocols ...
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bioRxiv - Genomics 2022Quote: ... All PCR reactions were performed using Q5® Hot Start High-Fidelity 2X Master Mix (NEB, M0494S). The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were ligated using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA) and transformed into competent DH5a E ...
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bioRxiv - Synthetic Biology 2022Quote: ... The two PCR fragments were then assembled using the HiFi Gibson Assembly kit from NEB (Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2019Quote: ... were assembled by PCR using the Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA) and the mCherry_KpnI_fw and mCherry_KpnI_rv primers (Supplementary Table 4) ...
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bioRxiv - Neuroscience 2019Quote: ... HDAC6 cDNA was generated from pCMV6-XL4-HDAC6 by PCR using Phusion High Fidelity DNA polymerase (NEB) with primers containing restriction sites (SalI-HDAC6-forward ...
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bioRxiv - Immunology 2019Quote: ... Polymerase chain reactions (PCR) for cloning steps were performed with Phusion High Fidelity polymerase (New England Biolabs). KHNYN-2 (NM_001290256 ...
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bioRxiv - Bioengineering 2019Quote: ... the DNA from the PCR reaction was purified using a clean-up kit (T1030, New England Biolabs) and sequenced using SimpleSeq™ tubes (Eurofins Genomics ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCRs for new constructs were performed using Q5 High Fidelity polymerase (New England Biolabs, Ipswich, Massachusetts, USA), and analytic PCRs were performed using DNA AmpliTools Green Master Mix (BioTools ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Endpoint PCR for genotyping or single guide RNA template preparation was carried out using Q5 (M0491 NEB) or Taq (M0267 NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... 50μL of the completed PCR reaction mixture was then digested with 2.5μL Dpn1 and 5.8μL CutSmart® Buffer (New England Biolabs) for 3h at 37°C to degrade methylated template DNA ...
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bioRxiv - Microbiology 2019Quote: ... PCR products and expression plasmid pJN105 (Newman and Fuqua, 1999) were digested with EcoRI and SacI (NEB) for 37°C for 1 hr ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR was carried out using Phusion® or Phusion U High-Fidelity DNA Polymerase (New England Biolabs) according manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Ligation and phosphorylation of PCR products were performed simultaneously using T4 DNA ligase (New England Biolabs, M0202L) and T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (kind gift from Jonathan Abraham lab) ...
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bioRxiv - Systems Biology 2020Quote: SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (gift of Jonathan Abraham) ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCR amplifications were carried out with the Q5® High-Fidelity DNA Polymerase (New England Biolabs) following the protocol recommended by the manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCR reaction were carried out using the Q5® High-Fidelity DNA Polymerase (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... An 8.9 kb fragment preceding and including exon 5 was amplified by PCR (Phusion DNA Polymerase, NEB) and cloned into a plasmid encoding both a floxed neomycin resistance cassette and a diphtheria toxin negative selection marker ...
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bioRxiv - Cell Biology 2020Quote: ... GRET and PalmGRET fragments were PCR amplified with Q5® High-Fidelity DNA polymerase (New England Biolabs) from pRetroX-Tight-MCS_PGK-GpNLuc (a gift from Antonio Amelio ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products used for sequencing or plasmid synthesis were performed with Q5 high-fidelity polymerase (NEB). The resulting DNA fragment was digested and ligated into NdeI-xhoI digested pET21b(- ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PCR product was digested with StuI restriction enzyme (NEB, New England Biolabs, Ipswich, MA, USA), according to manufacturer protocols ...
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bioRxiv - Plant Biology 2020Quote: ... followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs, USA) which was finally performed in 7500 Fast Real-Time PCR System (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs, USA) which was finally performed in 7500 Fast Real-Time PCR System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... one PCR product amplified from gBlock1 (JEP1862+JEP1863) and pMS26 digested with NotI using NEBuilder Hifi (NEB). pMTP114 was constructed by assembling two PCR products amplified from F plasmid (JEP1398+1340 and JEP1341+1399 ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCRs were performed with KOD-Plus DNA polymerase (Toyobo, Osaka, Japan) or Q5 DNA polymerase (NEB, Beijing). The one-step cloning method was employed in plasmid construction according to the manufacturer’s instructions (Vazyme ...
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bioRxiv - Immunology 2020Quote: ... and then Illumina barcoded and amplified with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). A portion of the reaction was performed as a separate qPCR reaction to determine ideal cycle number ...
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bioRxiv - Synthetic Biology 2020Quote: ... Transformants were screened by colony PCR using OneTaq 2x Master Mix (New England Biolabs, Ipswich, MA, USA). Production plasmids were isolated and analyzed by PCR and Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PCR product was digested with StuI restriction enzyme (NEB, New England Biolabs, Ipswich, MA, USA), according to manufacturer protocols ...
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bioRxiv - Molecular Biology 2021Quote: Genotyping was performed by Q5 PCR (0.5 μl Q5® HiFi DNA Polymerase (New England Biolabs, M0491), 1xQ5 reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The NOCT PCR insert and pcDNA5/FRT/TO were digested with BamHI and NotI restriction enzymes (NEB) before ligation with T4 DNA ligase (NEB) ...