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Citations for New England Biolabs :
5201 - 5250 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Mutant constructs were created and amplified via PCR with Q5® High-Fidelity DNA Polymerase (NEB), and the respective primers provided by NEBaseChanger (Synbio Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... the reaction was cleaned and concentrated with a Monarch PCR & DNA Cleanup Kit (NEB catalog # T1030L) and eluted in either 10 or 12 µL of nuclease free H2O ...
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bioRxiv - Genomics 2022Quote: ... The product was then purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L), and eluted with 20 μl of ddH2O ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs), which yield pDRF1-GW AKAR3-EV.
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bioRxiv - Evolutionary Biology 2022Quote: ... Fragments were amplified from the UCA860 sequence via PCR using Q5 Polymerase (NEB, Ipswich, MA, #M0491). The resulting fragments were purified using a 2X ratio of Aline beads (Aline Biosciences ...
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bioRxiv - Evolutionary Biology 2022Quote: All cassettes were designed and prepared by fusion PCR approach using Phusion polymerase (NEB Biolabs, M0530S) as described elsewhere (Faktorová et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: All cassettes were designed and prepared by fusion PCR approach using Phusion polymerase (NEB Biolabs, M0530S) as described elsewhere (Faktorová et al. ...
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bioRxiv - Plant Biology 2022Quote: ... pENTR_proMpTIR1 and the 3xFLAG-AtTIR1 PCR products were digested with AscI (New England Biolabs, Massachusetts, U.S.A.) and then ligated into pENTR_proMpTIR1 to generate pENTR_proMpTIR1:3xFLAG-AtTIR1 ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions (50 µl) contained 1.0 unit of Phusion DNA polymerase (New England Biolabs catalog # M0419), 1 × Phusion HF buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The target gene sequences were PCR amplified from the purchased plasmids using either Phusion (NEB, M0531S) or Q5 High Fidelity (NEB ...
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bioRxiv - Microbiology 2022Quote: ... and PCR enrichment (eight cycles) with NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA). DNA concentrations were estimated using qPCR and the Kapa Library Quantitation Assay for Illumina (Kapa Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... and 200 bp downstream of marR were PCR amplified using Q5 DNA Polymerase (New England Biolabs) and cloned into the DraI and HindIII sites of the integrative vector pMV306 (67) ...
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bioRxiv - Plant Biology 2022Quote: ... The specific cDNA was amplified by PCR using Phusion DNA polymerase (New England Biolabs, Ipswich, MA) and primers AtINOcDNA-f and AtINOcDNA-r (Supplemental Table S1) ...
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bioRxiv - Immunology 2022Quote: ... 10 μl PCR products were incubated with 1.5 μl 10X NEBuffer (New England Biolabs, Ipswich, MA) and 1.5μl Nuclease-free water with the thermal cycler setting ...
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bioRxiv - Molecular Biology 2023Quote: Purified samples were combined with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs M01541) for amplification ...
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bioRxiv - Plant Biology 2023Quote: ... Amplifications were performed using the High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) or the GoTaq G2 polymerase (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was amplified by PCR using the Q5 High-Fidelity DNA polymerase kit (New England BioLabs) following the manufacturer’s recommendations and with primers designed around the mutations (supplementary Table S9) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μl was used for PCR amplification using Q5 hot start high-fidelity polymerase (NEB #M0494S) and a unique combination of the dual-barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293) ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), and 300 ng of the cDNA and 10 µM of each the RSV5U and RTRHC primers ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthesized cDNA was purified using Monarch® PCR and DNA Cleanup Kit (New England Biolabs). Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... A 50 μL PCR reaction for the gene block was carried out with Phusion polymerase (NEB) using primers DGR_gblock_fwd and DGR_gblock_rev for 35 cycles (denaturing ...
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bioRxiv - Molecular Biology 2022Quote: ... the adaptor-ligated cDNA was processed to library PCR amplification using Phusion Master Mix (NEB, M0531L) with IDT adaptor indexes ...
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bioRxiv - Microbiology 2022Quote: Putative replicon fragments were obtained through amplification PCR using high fidelity enzyme Q5 DNA polymerase (NEB) and primers including a KpnI or NcoI restriction site (Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... Digested and purified PCR products were then phosphorylated and ligated simultaneously using T4 PNK Enzyme (NEB), T4 DNA Ligase (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB) site of broad host-range vector pBBR1MCS-5 (Kovach et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and amplified in 50 µl PCR reactions using Taq DNA polymerase (NEB, Cat. No. / ID: #M0273S) and primers 1154A.S8 and 1154A.S29 (1121bp) ...
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bioRxiv - Biophysics 2022Quote: ... miRFP670nano replaced EGFP and EGFPA206K mutated back into EGFP using PCR and Gibson Assembly System (NEB). Cell transfection was done with PEI reagent (24765-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Then the PCR amplicon of Cdk1 CpG was digested by KpnI and MfeI (New England Biolabs) to remove the overhangs ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were inserted into the respective plasmids using the NEBuilder HiFi DNA Assembly system (NEB). All constructs used in this study were confirmed by sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... comprising 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), 0.2 μM of forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Microbiology 2023Quote: ... The template was gel-purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Massachusettes, USA) and transformed into UKMCC1015 electrocompetent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-seq libraries were prepared using the NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) as previously described76 ...
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bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product and the vector were enzyme-digested using NdeI and XbaI (New England Biolabs) for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR products (∼300 ng) were incubated with 2 μl 10X NEBuffer 4 (New England Biolabs), 1 μl BtsCI restriction enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... The linearized vector was then ligated to the digested PCR product using a quick ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Homo sapiens cDNAs were amplified by PCR using Q5 High Fidelity DNA Polymerase (New England Biolabs) and sub-cloned into a variety of vector backbones as indicated in the figure legends ...
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bioRxiv - Microbiology 2024Quote: ... Flanking regions of the target genes were amplified via PCR using Phusion polymerase (New England BioLabs). All primers used in the study are listed in Table S7.
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bioRxiv - Evolutionary Biology 2024Quote: ... Successful amplicons were purified using the Exo-CIP Rapid PCR Cleanup Kit (New England BioLabs®) and sent for bi-directional Sanger sequencing with Eurofins Genomics.
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid obtained by PCR was ligated using the KLD enzyme mix (NEB cat # M0554S) according to the manufacturer’s protocol before transformation ...
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bioRxiv - Immunology 2024Quote: ... DNA was then amplified using NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) for 5 cycles ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2µg of resulting PCR products were then digested with Pst1-HF restriction enzyme (New England Biolabs) to cleave the unspliced XBP1 template ...
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bioRxiv - Cell Biology 2023Quote: ... Mutagenesis on Cas9 and cGAS was performed by conventional PCR followed by HiFi DNA Assembly (NEB) or In-Fusion cloning (Takara Bio) ...
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bioRxiv - Immunology 2023Quote: ... for mouse was PCR-amplified and ligated into this vector via Gibson assembly (NEB, Cat# E2621S). The ligated product was precipitated ...
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bioRxiv - Genomics 2023Quote: ... Homology arms were amplified from HaCat cell genomic DNA via PCR using Q5 DNA polymerase (NEB). The −loxP-PGK-Em7-NeoR-loxP- cassettes were amplified from PL452 (79) ...
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bioRxiv - Molecular Biology 2023Quote: ... The deaminated ΦX174 DNA was purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo plasmid was linearized via Polymerase Chain Reaction (PCR) with the Q5 DNA polymerase (NEB) and a pair of primers for each Cac1 mutant that anneal to the sequences flanking the section to be modified (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... HindIII/NotI-digested PCR reactant and backbone were ligated by T4 DNA ligase (New England Biolabs) and transformed to CloneCatcher™ (Genlantis ...