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Citations for New England Biolabs :
5301 - 5350 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Homology arms were amplified from HaCat cell genomic DNA via PCR using Q5 DNA polymerase (NEB). The −loxP-PGK-Em7-NeoR-loxP- cassettes were amplified from PL452 (79) ...
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bioRxiv - Immunology 2023Quote: ... for mouse was PCR-amplified and ligated into this vector via Gibson assembly (NEB, Cat# E2621S). The ligated product was precipitated ...
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bioRxiv - Microbiology 2023Quote: ... and 12.5 μL of master mix (2X Phusion High-Fidelity PCR Master Mix, New England Biolabs). The high ratio of exterior to interior primers helps ensure the dominant product is the desired full-length amplicon ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were gel-purified and assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB). The assembled product was transformed into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA). For ITS we used the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... The gRNA cassette was amplified using the NEBNext® High-Fidelity 2X PCR Master Mix (NEB) and primers including Illumina adaptors and 8nt barcodes to allow Next Generation Sequencing analysis (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... 2µg of resulting PCR products were then digested with Pst1-HF restriction enzyme (New England Biolabs) to cleave the unspliced XBP1 template ...
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bioRxiv - Cell Biology 2023Quote: ... Mutagenesis on Cas9 and cGAS was performed by conventional PCR followed by HiFi DNA Assembly (NEB) or In-Fusion cloning (Takara Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB) and InFusion cloning (Takara Bio) ...
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bioRxiv - Synthetic Biology 2023Quote: ... All colony PCRs were done using Quick-Load® Taq 2X Master Mix (New England Biolabs) according to the manufacturer’s recommendation ...
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bioRxiv - Genetics 2024Quote: ... and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: ... phi47 orf65 (tifA) and orf65-L32F were PCR amplified using Q5 master mix (New England Biolabs) and cloned into pLZ12A ...
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bioRxiv - Cell Biology 2024Quote: ... Doxycycline inducible CCDC134 constructs were cloned by PCR amplification followed by Gibson assembly (New England Biolabs) into pLenti-TRE- rtta3G-BLAST.
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bioRxiv - Genomics 2024Quote: ... and amplified with 14 cycles of PCR using LongAmp Taq Master Mix (New England Biolabs, UK). cDNA was quantified using the Qubit DNA High sensitivity assay (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed according to manufacturer directions using Q5 or OneTaq DNA Polymerases (NEB, Ipswitch, MA). DNA was digested with restriction enzymes from New England Biolabs (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... Purified PCR fragments were assembled using Gibson Assembly® Master Mix (New England Biolabs, MA, USA) following the manufacturer’s procedures.
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bioRxiv - Cell Biology 2024Quote: ... 100ng cDNA was amplified through a 30-cycle PCR protocol using OneTaq polymerase (New England BioLabs) and the primers indicated in Table S2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified error-prone PCR products were digested using BamHI-HF (New England Biolabs, MA, USA) and HindIII-HF (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... purified with tRNA-coated streptavidin beads and amplified with 2x Phusion high-fidelity PCR mix (NEB). CAGE libraries were treated with Exonuclease I (NEB ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were carried out using Phusion high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). Plasmids were digested using NEB high-fidelity restriction endonucleases in Cutsmart buffer and plasmid assembly reactions were performed with NEBuilder HiFi DNA assembly ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed in 25 μL reaction volumes using 12.5 μL of Taq 2X MasterMix (NEB), 9.5μL water ...
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bioRxiv - Cell Biology 2024Quote: ... Double digested PCR product and plasmid were further subjected to Ligation using T4 DNA ligase (NEB) at 16°C for 12-14 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The 50 µl PCR product with 10 µl of 6X Purple Loading Dye (New England Biolabs) was electrophoresed through a 50 ml gel – 1% SeaKem Agarose (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Molecular Biology 2024Quote: ... Inserts and fragments generated by PCR were synthesized using Q5 High Fidelity DNA Polymerase (NEB, M0491). All primers were synthesized by Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was then amplified in the first round of PCR using Q5 DNA Polymerase (NEB), and Illumina barcodes were appended during a second PCR ...
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bioRxiv - Genomics 2024Quote: ... The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB) with NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The digested PCR products were purified and ligated into NotI-digested and shrimp alkaline phosphatase (NEB) dephosphorylated pPL-RAG vector using T4 DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were digested using 0.2 μL of DpnI restriction enzyme (New England Biolabs, catalog #R0176L) (37 °C for 2 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then used as DNA template for PCR reactions using standard Taq DNA polymerase (NEB).
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bioRxiv - Microbiology 2020Quote: ... The cut plasmid and PCR product were then purified and ligated by T4 ligation (New England Biolabs). For constructing the pZKF2 (pBBR-ftsZGFP) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
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bioRxiv - Synthetic Biology 2021Quote: ... Linear DNA templates from IDT were amplified by PCR using Q5 High fidelity DNA polymerase (NEB, #M0491S) and dNTPs (NEB ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this PCR reaction was then combined with 5 μL Q5 buffer (NEB, cat#M0491S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were used as a template for RNA synthesis with T7 RNA polymerase (New England Biolabs) and purified (QIAGEN RNA purification kit ...
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bioRxiv - Cell Biology 2020Quote: ... We quantified the cDNA with NanoDrop and performed PCRs with Q5 Hot Start High Fidelity polymerase (NEB) using equal amounts of obtained cDNA from each tissue sample to detect presence of TMEM16K (primer set 1 ...
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bioRxiv - Cell Biology 2020Quote: ... The fragments for the assembly were prepared by PCR using Q5 High Fidelity polymerase (New England Biolabs). The plasmids used along with details on their preparation are listed in Supplementary Table 2.
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bioRxiv - Molecular Biology 2021Quote: ... two independent amplicons were generated by PCR using Q5 Hot Start High-Fidelity 2X Master Mix (NEB). One amplicon for the targeted locus and one amplicon of the pegRNA locus (primers listed in Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We did 12 rounds of high-fidelity PCR amplification (Phusion High-Fidelity DNA Polymerase, NEB, Ipswich, MA) using PCR primers that included one of 12 unique Illumina multiplex read indices (Supplementary Table S2) ...
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bioRxiv - Developmental Biology 2020Quote: ... Final library for paired-end sequencing was prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB). PCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... Final library for paired-end sequencing was prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB) and a nested PCR approach as described in Schwartzman et al ...
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bioRxiv - Genetics 2022Quote: ... We cleaned the PCR product with Exonuclease I and Antarctic Phosphatase (New England BioLabs, Ipswich, MA, USA) before sending to Eurofins Genomics (Louisville ...
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bioRxiv - Neuroscience 2021Quote: ... Site directed mutagenesis was performed using overlapping PCR with Phusion® High-Fidelity DNA Polymerase (NEB M0530S) and primer sequences mentioned in Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by re-circularization through PNK phosphorylation of PCR product ends and ligation by T4 ligase (NEB). Similarly ...
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bioRxiv - Molecular Biology 2020Quote: ... real time PCR was performed in triplicate using Luna® Universal qPCR Master Mix (New England Biolabs) with variable amounts of cDNA and 0.5 μM of target-specific primers in a CFX connect instrument (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... pri-miRNA sequences were amplified by PCR using Phusion® High-Fidelity DNA Polymerase (NEB BioLabs, USA) from human embryonic kidney (HEK293T ...