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Citations for New England Biolabs :
5501 - 5550 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... 25 μL of 2xKAPA NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Cat. M0543L), and 0.5μL of 100x SYBR Green I dye (FMC BioProducts ...
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bioRxiv - Plant Biology 2022Quote: ... the MpCNL1 (Mp3g01950,1-266aa) and MpCNL1ΔN (Mp3g01950,31-266aa) were cloned by PCR (Q5 High Fidelity Polymerase, NEB) with attL-containing primers using codon optimized gene fragments as a template ...
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bioRxiv - Plant Biology 2022Quote: ... PCR amplification spanning the gene deletion was achieved using Phusion High-Fidelity DNA Polymerase (New England Biolabs) according to the manufacturers protocol and visualized on 1% agarose gel supplied with ethidium bromide.
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions were run on 1.2% TAE agarose gel with a standard 1kb ladder (New England BioLabs) to confirm formation of products ...
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bioRxiv - Plant Biology 2024Quote: ... the respective promoter was isolated via PCR using Phusion High Fidelity Enzyme (New England Biolabs, no. M0530L) and inserted upstream of GUS gene of pCambia1301 vectors ...
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bioRxiv - Plant Biology 2024Quote: ... and the genes were PCR-amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... The DNA fragment carrying the cassette was obtained by overlap PCR using Q5 polymerase (New England Biolabs). For each target gene ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions followed the manufacturer recommended recipe for Phusion High Fidelity Polymerase (New England BioLabs, Inc.) with these PCR conditions ...
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bioRxiv - Microbiology 2024Quote: ... Size-selected cDNA was then PCR amplified for 12 cycles using Q5 High-fidelity polymerase (NEB #M0491S). Amplified libraries were then run on a 6% TBE gel ...
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bioRxiv - Biochemistry 2024Quote: Point mutants were generated using inverse polymerase chain reaction (PCR) with Phusion DNA polymerase (New England Biolabs) or Herculase II (Agilent) ...
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bioRxiv - Microbiology 2024Quote: LT area was amplified from 12-week gDNA with PCR using Q5 High-Fidelity DNA Polymerase (NEB) and cloned to pUC19 vector to BamHI/HindIII site ...
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bioRxiv - Microbiology 2024Quote: ... The resulting GlnA1_opt PCR-product and vector pRS375 were restricted with NdeI and BamHI (NEB, Schwalbach, Germany); the resulting pRS375 vector fragment and the GlnA1 fragment were ligated resulting in pRS1841 ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified using GeneAid and ∼100 ng of DNA subjected to digestion by NaeI (NEB). Clones that showed no apparent digestion product (due to the modification of the NaeI restriction site ...
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA and cDNA PCRs were carried out using OneTaq polymerase (New England Biolabs, Ipswich, MA, USA) as per instructions ...
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bioRxiv - Microbiology 2024Quote: ... The 3’ ends of the VSV G and GP64 ORFs were PCR-amplified (using Taq polymerase, NEB) from DNA isolated from each fly line ...
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bioRxiv - Evolutionary Biology 2024Quote: ... France) the amplicon obtained from PCR using Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and the primers Salten-pb1-3183-F / Salten-pb1-4136-R primers (TableS1) ...
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bioRxiv - Immunology 2024Quote: ... Transcript expression was analyzed via reverse transcriptase quantitative real-time PCR using Lunaprobe reagent (New England Biolabs) on a Quantstudio3 thermocycler (ABI) ...
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bioRxiv - Microbiology 2024Quote: All PCRs needed for cloning were carried out with high fidelity enzymes Q5 polymerase (New England Biolabs) or Accuzyme (Bioline) ...
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bioRxiv - Bioengineering 2024Quote: All polymerase chain reaction (PCR) amplification steps were performed using Phusion DNA polymerase (NEB, Whitby, Ontario, Canada). Primers used for PCR amplification were ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR reactions were performed with Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in 20 µl reactions and products were run on a 1% agarose gel alongside an appropriately sized ladder (either New England Biolabs 100 bp or 1kb DNA ladder ...
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bioRxiv - Bioengineering 2024Quote: ... 0.5 % Tween-20 and the selected genes amplified by PCR using Q5® Hot Start polymerase (NEB) using forward primer ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments of the nucleosome library present in each section were amplified via PCR (NEB Taq Polymerase) by using as primers the sequences of the Asc1 and BamH1 adapters described in Supplementary Fig ...
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bioRxiv - Biochemistry 2022Quote: ... PCR reactions were carried out using Q5 High Fidelity Hot Start DNA Polymerase (New England Biolabs (NEB), UK) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR reactions were carried out using Q5 High Fidelity Hot Start DNA Polymerase (New England Biolabs (NEB), UK) ...
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bioRxiv - Microbiology 2023Quote: ... Each fragment was amplified by PCR using the Q5® High-Fidelity DNA Polymerase (NEB, Cat #: M4091L) following the manufacturers protocols ...
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bioRxiv - Genetics 2023Quote: ... gRNA templates were PCR amplified and gRNAs were in vitro transcribed with T7 transcriptase (NEB, cat# M0251S). gRNAs and Cas9 protein (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and quick change PCR reactions were performed using Vent DNA polymerase (purchased from New England Biolabs, NEB) and oligonucleotide primers purchased from Eurofins Genomics ...
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bioRxiv - Synthetic Biology 2023Quote: ... and quick change PCR reactions were performed using Vent DNA polymerase (purchased from New England Biolabs, NEB) and oligonucleotide primers purchased from Eurofins Genomics ...
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bioRxiv - Molecular Biology 2023Quote: The coding sequence of PolA without the start codon was amplified by PCR using Q5 polymerase (NEB) and cloned BamHI-XhoI into pET28a (Thermo ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were performed using OneTaq Quick Load 2X Master Mix with Standard Buffer (New England Biolabs). Detection of Mincle-specific cDNA was conducted by PCR with the same gene-specific primers described in the previous section (Suppl ...
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bioRxiv - Microbiology 2023Quote: ... PCR product was assembled with EcoRI-linearized pBAD L24 using NEBuilder HiFi DNA Assemly Master Mix (NEB). Strep-Tag II and His-tag C-terminally modified SAMase coding plasmids were constructed in a similar manner ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed using OneTaq 2ξ Master Mix or Q5 High-Fidelity 2ξ Master Mix from NEB according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: PCR reactions were performed using NEB EpiMark Hot Start Taq DNA Polymerase (New England Biolabs, catalog #M0490L). The following program was used for all bisulfite-converted genomic DNA PCRs ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-free libraries were prepared with the NEBNext UltraTM II DNA Library Prep Kits (New England BioLabs) according to supplier recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The whole genomic sequence of TgAPT1 (www.toxodb.org accession number TGME49_261070) was amplified by PCR with the Q5 High Fidelity polymerase (New England Biolabs) from Pru genomic DNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then amplified by performing 20 cycles of PCR using Q5® High-Fidelity 2X Master Mix (NEB) (final concentration between 30-100 ng.µl-1) ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral fragments encompassing the pre-miRNA sequence were amplified by PCR using the High-Fidelity MasterMix (NEB) and 400 ng genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using OneTaq Quick-Load 2X Master Mix with Standard Buffer (New England Biolabs, USA). DNA assembly was done by using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... rLCMV-GP1-FLAG was generated by insertion PCR utilising Q5 site-directed mutagenesis kit (New England BioLabs) and FLAG primers ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Frankfurt a.M., Germany) and subse-quently ligated into the vector system pCR-Blunt II-TOPO via the Zero Blunt™ TOPO™ PCR cloning kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of MS2×128-containing lines was further confirmed by PCR using Q5 (New England Biolabs) and Terra (Takara ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified using a reaction mixture containing 2X NEB PCR Master Mix (NEB M0541L), a universal forward primer i5 ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using the high-fidelity polymerase Q5 (New England Biolabs - NEB M0492 or M0493) and purified using a DNA clean and concentrate kit (Zymo Research #D4014) ...
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bioRxiv - Genetics 2023Quote: ... The PCR products containing the geneticin128-795-TtrpCand LB border were digested using AgeI (New England Biolabs) followed by a ligation reaction ...
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bioRxiv - Microbiology 2023Quote: ... was amplified in PCR using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) using primers flanked with SfiI restriction enzyme recognition sequence (underlined ...
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bioRxiv - Bioengineering 2023Quote: ... then was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs, Catalog #M0493S), and subcloned into a bacterial expression vector (Addgene plasmid #29656 ...
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bioRxiv - Cancer Biology 2022Quote: ... All PCR reactions were carried out using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). pCL-ECO was used to generate retroviral virions ...