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Citations for New England Biolabs :
5601 - 5650 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... A 673 bp digoxigenin-labeled DNA handle was amplified in Q5U PCR master mix (NEB, Cat# M0597L) with a forward primer containing a deoxyuridine and a reverse primer with a 5′ digoxigenin tag ...
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bioRxiv - Synthetic Biology 2024Quote: ... the barcode and sensor regions of the biosensor libraries were amplified using Q5 PCR (New England Biolabs) directly off 5 μL of glycerol stock using primers p117 and p118 (Table 2) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were then cloned into SalI and NotI sites of a pMAL-C5X vector (New England Biolabs). All constructs were validated by DNA sequencing.
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bioRxiv - Genetics 2021Quote: ... NbVHH05/Nb127D01 PCR fragments were cloned into pET-26b-Nb-GGA by BsaI-Golden Gate Assembly (NEB, E1601).
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bioRxiv - Genetics 2021Quote: ... PCR reactions were performed with Phusion High Fidelity DNA Polymerase following the manufacturer’s instructions (NEB – New England Biolabs). All primers used in this study are available in Supplementary Table S1.
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned up using Exo-AP treatment (7.5 µl PCR product mixed with 0.5 µl Exonuclease-I (NEB), 0.5 µl Antarctic Phosphatase (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The 2.1 kbp region upstream of cas1 was amplified by PCR using Phusion DNA polymerase (New England Biolabs) and primers 2322 (adding a KpnI site ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNAs were used as templates in PCR amplification using Phusion High-fidelity DNA Polymerase (New England Biolabs). PCR products were individually treated for 16 h at 37 °C with 20 units of DpnI enzyme (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... Captured libraries were re-amplified using Post LM-PCR oligos (Nimblegen) and Q5 High-Fidelity DNA polymerase (NEB) directly from the beads ...
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bioRxiv - Developmental Biology 2021Quote: ... The correct sized fragments were amplified by PCR using PCRBio Ultramix (PCRBiosystems) and ligated into the pTK_JC_EGFP plasmid (Chen & Streit, 2015) using T4 ligase (NEB). Ligation products were transformed into DH5α E.coli and grown on agar plates with Ampicillin selection ...
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bioRxiv - Developmental Biology 2021Quote: ... This PCR product was cloned into pUC19 backbone using NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA). The assembled plasmid was microinjected at 25 ng/μl concentration ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned using 1U of Exonuclease I and Antarctic Phosphatase (New England Biolabs, Ipswich, Mass., USA) and incubating at 37 °C for 20 min followed by 10 min enzyme denaturation at 80 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Products from the first round PCR were then purified using Monarch DNA Gel Extraction Kit (New England Biolabs) and used as the templates for the second round insert PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... SEI1 mutants were generated in pBMF1 using appropriate PCR products and NEBuilder HiFi DNA Assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was digested with NdeI and EcoRI and was ligated to pMAL-c5X (NEB, Inc, USA). Escherichia coli (E ...
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bioRxiv - Molecular Biology 2020Quote: ... and excess adaptors were removed by magnetic beads (HighPrep PCR, Magbio) before and after amplification (Phusion polymerase, NEB).
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bioRxiv - Bioengineering 2022Quote: One μL of amplicons from colony PCR is mixed with 1 μL of Gel Loading Dye (6x; NEB) and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Pooled Illumina amplicon sequencing was performed with PCR amplifications using Q5 polymerase (New England Biolabs, catalog no. M0493L) (see Supplementary Methods for primer details) ...
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bioRxiv - Genomics 2020Quote: ... PCR was performed using cDNA template and Phusion High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the RNA-seq libraries were amplified via emulsion PCR [50] using the Phusion DNA Polymerase (New England Biolabs). The following primers containing Illumina adapters were used in the PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of PCR product of RFLP_region 1 and RFLP_region 2 were digested with PstI (New England Biolabs) and VspI (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... For the 16S enrichment we performed a PCR with Q5® High-Fidelity DNA Polymerase (New England Biolabs), the forward primer 341F and the reverse primer 806R to cover the V3/V4 region ...
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bioRxiv - Microbiology 2019Quote: ... sgRNA oligonucleotides were annealed and inserted into AflII-digested pCR-BluntII-Topo by Gibson Assembly (New England Biolabs). To generate the IRF3 CRISPR guide RNA plasmids ...
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bioRxiv - Genomics 2019Quote: ... The amplicons were size-selected (∼600 bp) and cloned using the NEB PCR Cloning Kit (New England Biolabs). The resulting amplicons were Sanger-sequenced at Eurofins Genomics (Cologne ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each 8-sample pool was then Qbit quantified and amplified using Phusion high-fidelity PCR (New England Biolabs) with a PCR primer with one of three unique barcodes ...
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bioRxiv - Synthetic Biology 2019Quote: ... by digesting both pCTCON2-RXG and the PCR-amplified BFP gene with EcoRI-HF and BamHI-HF (NEB), then ligating with T4 DNA ligase (NEB ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We then used a standard PCR reaction (Table S7; Q5 High Fidelity 2X Master Mix, New England Biolabs) to amplify DNA from an E ...
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bioRxiv - Neuroscience 2019Quote: ... We PCR amplified two fragments carrying three independent guides for PDFR using Q5-polymerase (New England BioLabs, NEB) and performed a Gibson assembly (NEB) ...
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bioRxiv - Neuroscience 2019Quote: ... We PCR amplified two fragments carrying three independent guides for PDFR using Q5-polymerase (New England BioLabs, NEB) and performed a Gibson assembly (NEB) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This fragment was generated by a standard 3-step PCR protocol using Phusion DNA polymerase (New England Biolabs) and then cloned into the XbaI and HindIII sites of pEX18A (Prentki and Krisch ...
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bioRxiv - Cancer Biology 2019Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (New England Biolabs Next UltraII, M5044S). i5 and i7 multiplexing barcodes were added in a second round of PCR and final gel-purified products were sequenced on Illumina NextSeq500 systems to determine sgRNA representation in each sample.
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bioRxiv - Synthetic Biology 2020Quote: DNA amplification was performed via PCR using the Q5 DNA polymerase and 5X Q5 buffer (New England Biolabs) in 50 µl ...
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bioRxiv - Microbiology 2019Quote: ... upstream and downstream regions of about 750 bp flanking the bspL gene were amplified by PCR (Q5 NEB) from B ...
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bioRxiv - Genomics 2020Quote: ... Strand extension reaction was performed by directly adding 0.32 U/μl Bst 3.0 DNA Polymerase and 1X NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB) to tagmentation products and incubating at 72°C for 15 min ...
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bioRxiv - Genetics 2020Quote: Unless indicated otherwise PCRs were performed with the Q5 Hot-start 2x master mix (New England Biolabs (NEB)) and cloning was performed using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Genetics 2020Quote: Unless indicated otherwise PCRs were performed with the Q5 Hot-start 2x master mix (New England Biolabs (NEB)) and cloning was performed using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: Donors bearing the desired mutations were generated by overlap extension PCR using Q5 polymerase (New England Biolabs M0492S) Given the high efficiency of homologous recombination following the generation of a defined double strand break ...
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bioRxiv - Neuroscience 2020Quote: ... The DNA insert and the hCPEB3 IDR PCR product were digested with XhoI and BamHI restriction enzymes (NEB) and cloned into a pEGFP-C1 expression vector ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR amplification were performed using the Truseq Small RNA Sample Prep Kit for Illumina (NewEngland Biolabs, E7335S) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... were amplified by PCR and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Biochemistry 2020Quote: ... The DNA was immediately purified using a DNA cleanup column (the Monarch PCR & DNA cleanup kit from NEB) and eluted with water ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB).
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB). The specificity of AlkB repair reaction was confirmed using an inactive AlkB control reaction in which Fe2+ ...
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bioRxiv - Evolutionary Biology 2019Quote: ... All PCR reactions were carried out in duplicate using Taq DNA Polymerase and ThermoPol Buffer (New England Biolabs). We used (RT negative ...
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bioRxiv - Cancer Biology 2021Quote: ... Gel-purified PCR product was then cloned into the pGL4.23 vector using T4 DNA ligase (New England Biolabs). Finally ...
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bioRxiv - Biophysics 2021Quote: ... Other constructs were cloned by PCR and DNA assembly (NEBuilder HiFi DNA Assembly Master Mix; New England Biolabs). The RGG domain used here is the N-terminal IDR (residues 1-168 ...
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bioRxiv - Cell Biology 2021Quote: ... and the polymerase chain reaction (PCR) was used to amplify DNA fragments with Phusion polymerase (New England Biolabs) of the genomic sequence using a forward primer that starts from 2199 bases upstream of the ATG start codon of zipt-2.3 and a reverse primer that contained the codon preceding the stop codon of zipt-2.3 and the coding sequence of the T7 epitope (MASMTGGQQMG) ...
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bioRxiv - Neuroscience 2019Quote: ... Ten microliters of PCR products were digested with Nsi1 (3 hours with 5 units of Nsi1 enzyme (NEB)) and then ...
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bioRxiv - Biochemistry 2020Quote: ... PCR was carried out under standard conditions using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). Samples were kept at 98 °C for 30 s (1 cycle) ...
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bioRxiv - Cell Biology 2021Quote: ... PCR reactions for amplicon generation were carried out with Q5 High-Fidelity DNA Polymerase (NEB, cat. no. M0491). pCineo_EGFP_Giant_CT ...