Labshake search
Citations for New England Biolabs :
5351 - 5400 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... pri-miRNA sequences were amplified by PCR using Phusion® High-Fidelity DNA Polymerase (NEB BioLabs, USA) from human embryonic kidney (HEK293T ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified vector was ligated with the IRES-TagBFP PCR amplified product using Gibson assembly cloning (NEB). The sequences of the resulting plasmid clones were confirmed by Sanger sequencing.
-
bioRxiv - Genomics 2020Quote: ... DNA libraries were amplified by PCR for 6-10 cycles with Phusion HF DNA Polymearse (NEB, M0530) using Illumina TruSeq indexing primers for multiplexing ...
-
bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Q5 hot start DNA polymerase from New England Biolabs (NEB). PCR products were cleaned with the DNA Clean & Concentrator kit (Zymo Research) ...
-
bioRxiv - Bioengineering 2019Quote: ... PCR amplified samples were purified (AMPure XP beads) and added to prewashed streptavidin magnetic beads (NEB #S1420S) (wash and bind buffer ...
-
bioRxiv - Microbiology 2019Quote: ... PCR products and vector pBRCS12 (Wadler & Vanderpool, 2009) were digested with HindIII and BamHI (New England Biolabs) restriction endonucleases ...
-
bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
-
bioRxiv - Developmental Biology 2019Quote: ... lin-29a and lin-29b were amplified by PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S) from purified genomic wild-type C ...
-
bioRxiv - Bioengineering 2019Quote: ... All PCR amplifications were performed using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, Cat: M0494S) unless specified ...
-
bioRxiv - Microbiology 2019Quote: ... Deletion of the fliC gene was confirmed by colony PCR using OneTaq DNA polymerase (NEB, Hitchin, U.K.) and primers 372F ...
-
bioRxiv - Microbiology 2019Quote: ... A total amount of 25 ng cDNA was used in a standard PCR using Taq-Polymerase (NEB; number of cycles for product amplification ...
-
bioRxiv - Neuroscience 2019Quote: ... The nested PCR provided an approximate equivalent amplification needed for T7 Endonuclease I (T7E1, NEB; United States). The resulting digest was visualized on a 1.5 % agarose gel ...
-
bioRxiv - Molecular Biology 2019Quote: ... was introduced via PCR overlap extension using Gibson Assembly® Master Mix (New England Biolabs cat. #E2611), following the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Individual parts were ordered as gBlocks (IDT) or PCR amplified (NEB Q5 High-Fidelity 2x Master Mix). PCR products were purified with a GeneJET PCR Purification Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... bovis C9.1 line genomic DNA (gDNA) was PCR-amplified with Phusion polymerase (New England Biolabs; Beverley, MA), using primers specific to unique sequences at nt 2,592,052-2,592,072 (DA170 ...
-
bioRxiv - Microbiology 2019Quote: ... PCR was performed on a plasmid template using the Q5 High-fidelity 2× Master Mix (NEB, M0492L), with primers at final concentration of 500nM ...
-
bioRxiv - Immunology 2019Quote: ... PCR amplicons were then used for library preparation using NEBNext DNA Library Modules for Illumina platform (NEB). The final libraries were quantified using KAPA library quantification kit for Illumina (KAPA Biosystems) ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA, #M0530L) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
-
bioRxiv - Bioengineering 2021Quote: ... GFP and taCA were cloned by polymerase chain reaction (PCR) using pMAL-c5X (New England Biolabs, USA), pGEX-4T-1 (GE Healthcare ...
-
bioRxiv - Bioengineering 2019Quote: ... whereas colony PCRs were performed using 2xOneTaq Quick-Load Master Mix with Standard Buffer (New England Biolabs). Genomic DNA was extracted from overnight cultures using Yeast DNA Extraction Kit (Thermo Scientific) ...
-
bioRxiv - Microbiology 2021Quote: PCR amplifications were performed using OneTaq Quick-Load 2X Master Mix (New England BioLabs, Ipswich, MA, USA), 200 nM of each primer ...
-
bioRxiv - Cell Biology 2020Quote: ... Multiplex quantitative PCR was then performed using the Luna Universal Probe qPCR Master Mix (ref M3004, NEB), a Roche LightCycler or Rotor Gene Q real-time PCR (Qiagen ...
-
bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
-
bioRxiv - Bioengineering 2021Quote: ... the PCR reaction was purified via Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). Plasmids were constructed via the Hot Fusion Assembly Method (Fu et al. ...
-
bioRxiv - Immunology 2021Quote: ... Remaining variants of BRD9 were generated by overlap-extension PCR using Q5 Hot Start High-Fidelity (NEB). TurboID-V5 constructs were generated by first subcloning TurboID from an existing vector (Addgene plasmid #10716949 ...
-
bioRxiv - Bioengineering 2020Quote: ... Positive clones were confirmed for the genotype by colony PCR using Q5 Polymerase enzyme (NEB, Ipswitch, MA). The dCpf1/CRISPRi system was adapted for use in P ...
-
bioRxiv - Neuroscience 2021Quote: ... The open reading frame was PCR-amplified (Phusion high-fidelity DNA polymerase, New England Biolabs Cat #M0530) using primers Irk1-F and Irk1-R (Supplemental Table 7) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The PCR product was then end-repaired to produce blunt ends using T4 DNA polymerase (NEB M0203L), DNA Polymerase I ...
-
bioRxiv - Cancer Biology 2020Quote: ... The purified PCR products were denatured and reannealed in NEBuffer 2 (New England BioLabs, Ipswich, MA, USA), using a thermocycler ...
-
bioRxiv - Cell Biology 2021Quote: ... All ORF6 mutants were generated by PCR amplification using Phusion high fidelity DNA polymerase (NEB, Ipswich, MA) and overlapping PCR to introduce the indicated mutations ...
-
bioRxiv - Cell Biology 2021Quote: ... The PCR product was restricted with 66.7 U/ml AluI (R0137, New England Biolabs, Ipswich, MA, USA) in CutSmart buffer (New England Biolabs ...
-
bioRxiv - Plant Biology 2021Quote: ... Gel-extracted PCR products were assembled in a cut-ligation reaction via BsaI-HFv2 (New England Biolabs) into vector pRRES208.482 (kindly provided by Alison Huttly ...
-
bioRxiv - Plant Biology 2021Quote: ... The cDNA was used as a template for quantitative PCR using Luna Universal qPCR Master Mix (NEB) and a Viia7 real-time PCR instrument (Life Technologies/ABI) ...
-
bioRxiv - Plant Biology 2021Quote: ... and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA Polymerase (New England Biolabs). Libraries were pooled and sequenced (paired-end 2×150bp ...
-
bioRxiv - Microbiology 2020Quote: ... PCR reactions were performed with the Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs) with 100 ng of genomic DNA ...
-
bioRxiv - Microbiology 2021Quote: ... and 20 ng of mRFP PCR products were incubated with Gibson master mix (New England Biolabs, #E2611S) at 50°C for 1 hour ...
-
bioRxiv - Genomics 2021Quote: ... Carrier plasmid and the PCR product from the last step were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and were purified for ligation ...
-
bioRxiv - Microbiology 2021Quote: ... Two microlitres of this reaction was then used as template for PCR using Taq DNA polymerase (NEB), DNA oligo E4 (CSO-4720) ...
-
bioRxiv - Microbiology 2020Quote: ... The digested hemA PCR product and pHSG396 were ligated together using T4 ligase (New England Biolabs, US), at a 3:1 insert to vector mass ratio ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Endpoint PCR for genotyping or single guide RNA template preparation was carried out using Q5 (M0491 NEB) or Taq (M0267 NEB ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR was performed using a Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) and the cycling conditions were ...
-
bioRxiv - Microbiology 2020Quote: ... aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII ...
-
bioRxiv - Microbiology 2019Quote: ... The DNA was isolated from the supernatant using the PCR clean-up kit (New England Biolabs®) and quantified using a nanodrop ...
-
bioRxiv - Cell Biology 2020Quote: ... Nested PCR was then carried out using OneTag DNA polymerase with GC buffer and Q5 polymerase (NEB) for genomic DNA and cDNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 12.5 µl of Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Catalog no. M0531S) 0.8 µM of each primer (final concentration ...
-
bioRxiv - Cancer Biology 2022Quote: ... sgRNA sequences were then PCR-amplified using Q5 Hot Start High-Fidelity 2X Master Mix (M0494, NEB), barcoded primer pairs (see Supplemental Table 2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 200 mg of DNA per condition were PCR amplified using Q5 high fidelity DNA polymerase (NEB Inc.) for 16 cycles using primers (Forward ...
-
bioRxiv - Cell Biology 2022Quote: ... All purified DNA (~20 µl) was used for PCR amplification with NEBNext High Fidelity 2x MasterMix (NEB) and optimum cycle number was determined by qPCR ...
-
bioRxiv - Biochemistry 2022Quote: ... The amplified PCR product was digested in one step using NdeI and BamHI HF (New England Biolabs) and ligated into similarly treated pET-15b to create plasmid pET15b-EcGhrA ...