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Citations for New England Biolabs :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10-beta (NEB # C3019H). For vectors containing the ccdB/CmR cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced on an Illumina NextSeq 500 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: Level 0 entry vectors were created through a scaled down NEBuilder (NEB, Ipswich, MA) reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621S), NEBridge Ligase Master Mix (NEB # M1100S) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
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bioRxiv - Molecular Biology 2024Quote: All consumable enzymes for restriction ligation and Gibson assembly were procured from NEB (New England Biolabs, Ipswich, MA) including BsaI-HF-v2 (NEB #R3733S) ...
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bioRxiv - Molecular Biology 2024Quote: ... SEGS-1 region N was generated by cleaving the Kpn1 site using the Q5-site directed mutagenesis protocol (New England Biolabs, Ipswich, MA) from S1-1.5a (pNSB1829 ...
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start Taq DNA Polymerase kit (New England Biolabs) was used according to the manufacturer’s protocol to amplify the SEGS-1 region from DNA samples collected from 4 plants of each genotype ...
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bioRxiv - Molecular Biology 2024Quote: ... They were synthesized by an in vitro reaction with T7 RNA polymerase (New England Biolabs) as described previously 24 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed three times with buffer B (1.2 M sorbitol and 100 mM potassium phosphate buffer pH 7.5) and resuspended in 500 μL of spheroplast buffer (buffer B containing 20 mM VRC (Ribonucleoside–vanadyl complex NEB #S1402S), and 25 U of Lyticase enzyme (Sigma #L2524 ...
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bioRxiv - Molecular Biology 2024Quote: ... which was then used to construct the IP library following the protocol (NEB, USA). The input library was constructed without the antibody treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBridge Ligase Master Mix (NEB # M1100S), and cloning strains of E ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified fragment was added to pWM530 digested with the same enzymes and ligated using T4 DNA ligase (NEB) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were constructed using NEBuilder HiFi DNA Assembly or USER cloning (New England Biolabs) and purified with the Zyppy Plasmid Miniprep Kit (Zymo).
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bioRxiv - Molecular Biology 2024Quote: ... Chromatinized DNA (100 ng) was digested using MNase (NEB) in 25 µL reactions with 2.5–25 units of enzymes that was incubated for 5 minutes at 37 °C before 1 mM of CaCl2 was added and incubation continued for another 5 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µL of CutSmart Buffer (NEB #B6004S) for one hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid was then digested with EcoRI and EcoRV (NEB) overnight to generate linear chromatin templates (i.e. ...
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bioRxiv - Molecular Biology 2024Quote: ... including DH5α (NEB #C2987H) and 10-beta (NEB # C3019H) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.25 U of Hotstart Taq Polymerase (New England Biolabs), 10 µM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: All consumable enzymes for restriction ligation and Gibson assembly were procured from NEB (New England Biolabs, Ipswich, MA) including BsaI-HF-v2 (NEB #R3733S) ...
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bioRxiv - Molecular Biology 2024Quote: ... MNase (NEB) was added to 2 x 106 nuclei in 50 µL reaction volume with 250– 2000 units of enzyme that was incubated for 5 minutes at 37 °C before 1 mM of CaCl2 was added and incubation continued for another 5 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: Replication deficient adenovirus plasmids were linearized using PacI enzyme (NEB, Cat# R0547) as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Inserts and fragments generated by PCR were synthesized using Q5 High Fidelity DNA Polymerase (NEB, M0491). All primers were synthesized by Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated DNA was washed once with 70% ethanol and suspended in 30μl 0.1 X TE (1mM Tris-HCl and 0.1mM EDTA) for library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Ligation of Unique Dual Index UMI Adaptors (NEB). Barcoding PCR was consistently carried out with NEBNext High-Fidelity 2X PCR Mix and subsequent size selection using agarose gel or beads (Ampure XP) ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 2X B&W buffer supplemented with 0.4 U/µL RNase Inhibitor (Murine) (NEB, M0314S) to a bead concentration of 5 ug/µL ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBNext Ultra II Q5 Master Mix (NEB, M0544S) in a 50 µL final reaction volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Final vectors were assembled using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create pGL3 plasmids containing VEGFAe ...
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bioRxiv - Molecular Biology 2024Quote: Omni-ATAC or CUT&Tag libraries assembled into the pLenti-STARR vector using HiFi assembly (NEB, E5520S), as previously described [Arnold et al ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed using NEBNext Polymerase (NEB) using the primer sets listed in Supplementary Table 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using ProtoScript II Reverse Transcriptase (New England Biolabs #M0368L) and poly(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... and ATP and m7GpppAm cap analog (New England Biolabs) at a 1:5 ratio.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM phosphoenolpyruvate and 0.15 mg/ml bovine serum albumin (New England Biolabs), in a final volume of 15 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB, S1402S], 20 μM beta-mercaptoethanol) and digested with 0.002% 100 T zymolyase (US Biological ...
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bioRxiv - Molecular Biology 2024Quote: ... The oligonucleotide-based DNA substrate used for the helicase and nuclease assays was generated by labeling the BIO100C oligonucleotide (see Table S1 for oligonucleotides sequence) at the 3’ end using terminal transferase (New England Biolabs) and [α-32P]dCTP (Hartmann Analytic ...
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bioRxiv - Molecular Biology 2024Quote: ... 1µg fragment DNA was end repaired using NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546). PCR adapters from Oxford Nanopore Technologies (ONT #SQK-PSK004 ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103). DNA was sequenced on Illumina HiSeq 2500 platform.
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bioRxiv - Molecular Biology 2024Quote: ... 3.2 U/ml Nt.CviPII (NEB #R0626S) and 64 U/ml E ...
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bioRxiv - Molecular Biology 2024Quote: ... This DNA was subjected to 18 cycles of PCR using whole genome primers (ONT #SQK-PSK004) and LongAmp Hot Start Taq 2X Master Mix (NEB #M0533). Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645 ...
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bioRxiv - Molecular Biology 2024Quote: ... and joining SEGS-1F and SEGS-1G using the indicated primers in Supplementary Figure 1 and the Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). All the primers except for those for site-directed mutagenesis added NotI restriction sites at the 5’ and 3’ends of PCR products ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared from 20 uL of ChIP DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Library molarity and quality were assessed by Qubit and Tapestation (DNA High sensitivity chip) ...
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bioRxiv - Molecular Biology 2024Quote: ... m6A-RIP mRNA was purified using the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were eluted in 8 µl elution buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB, E6150S) and purified using Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs), profiled using the Agilent TapeStation D1000 high sensitivity ScreenTape on the Agilent 4150 TapeStation System ...
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bioRxiv - Neuroscience 2024Quote: ... we synthesized (EnGen, NEB E3322S) and purified (Zymo R1018 ...