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Citations for New England Biolabs :
5151 - 5200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... using Q5 High-Fidelity DNA Polymerase (New England BioLabs). Diagnostic PCRs were performed using Thermo Dream TaqTM PCR ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). The percentage of infected cells was calculated using an open-source Fiji macro (83).
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: WMS library prep was carried out using Covaris focused-ultrasonication for fragmentation followed by NEB for indexing ...
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... or Q5 high fidelity polymerase (New England Biolabs; NEB) utilizing NF54 genomic DNA as template ...
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bioRxiv - Microbiology 2024Quote: ... The qPCR was performed with Luna Universal qPCR Master Mix from NEB and primer sets for gyrB (gyrB_Fwd and gyrB_Rev) ...
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bioRxiv - Microbiology 2024Quote: ... the template vector was linearized with the restriction enzymes EcoRI-HF (NEB, #R3101L) and BamHI-HF (NEB ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA isolated from recombinant parasites was digested to completion by the restriction enzymes XbaI and BglII (PfSUN1-HA-glms) and XbaI and SacI (PfSUN2-HA-glms) (NEB) and subjected to gel electrophoresis using 1% Agarose in Tris/Borate/EDTA (TBE) ...
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bioRxiv - Microbiology 2024Quote: ... pLVX-EF1alpha was constructed using Q5 Site-Directed Mutagenesis (NEB, #E0554S) according to the manufacturer’s instructions with pLVX-EF1alpha-SARS-CoV-2-ORF8-2xStrep-IRES-Puro (codon-opt.HUM ...
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bioRxiv - Microbiology 2024Quote: Libraries for whole transcriptome sequencing were produced from 20 ng total RNA per sample using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and its corresponding sample indexing kit (Index Primer Set 1) ...
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bioRxiv - Microbiology 2024Quote: ... Equimolar ratios of each annealed pegRNA pieces were phosphorylated with T4 polynucleotide kinase (New England Biolabs). The annealed and phosphorylated pieces were ligated with the pPEPZ_pegRNA digested vector with T4 DNA ligase ...
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bioRxiv - Microbiology 2024Quote: ... or Q5 high fidelity polymerase (New England Biolabs; NEB) utilizing NF54 genomic DNA as template ...
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bioRxiv - Microbiology 2024Quote: ... Enzymes were purchased from these companies: High-fidelity Q5 polymerase (New England Biolabs), GoTaq polymerase (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and restriction enzymes (New England Biolabs). Engineered strains and plasmids underwent initial PCR screening and were finally validated by Sanger sequencing of PCR-amplified fragments or plasmids.
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR fragments were combined to generate plasmid pTkv141 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs), and transformed into chemically competent DH5 E ...
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bioRxiv - Microbiology 2024Quote: ... while OneTaq polymerase (New England Biolabs) was used for screening of plasmids and mutant strains ...
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bioRxiv - Microbiology 2024Quote: ... Cloning fragments were amplified with Q5 high-fidelity polymerase (New England Biolabs, Ipswich, MA, USA), while OneTaq polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... primers TZ-54 and TZ-55 were used to amplify the C-terminus of CapRelSJ46 using Taq polymerase (NEB) and 0.5 mM MnCl2 was added to the reaction as the mutagenic agent ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... coli RNase H (New England Biolabs) was then used to initiate a sequence-specific rRNA cleavage in 10 µL final volume ...
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bioRxiv - Microbiology 2024Quote: ... 2 U of quick CIP (NEB), and water as needed ...
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bioRxiv - Microbiology 2024Quote: ... All reactions were supplemented with 0.8 U/µL RNase Inhibitor Murine (NEB, M0314S). Purified His6-MBP-tagged CapRelSJ46 protein was added to the reaction at a final concentration of 500 nM ...
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bioRxiv - Microbiology 2024Quote: ... Both genes were cloned separately into the pPink-HC expression vector multiple cloning site (MCS) using EcoRI and FseI (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2024Quote: ... Both genes were cloned separately into the pPink-HC expression vector multiple cloning site (MCS) using EcoRI and FseI (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2024Quote: ... All PCR steps were carried out with Phusion polymerase (NEB) using the manufacturer’s guidelines.
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bioRxiv - Microbiology 2024Quote: ... The P1 expression plasmid was linearised by SacII (NEB), and the 3CD PCR product inserted ...
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bioRxiv - Microbiology 2024Quote: ... The resulting plasmid was linearised by AflII digestion (NEB) and transformed into PichiaPink™ Strain one (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... using the 2X Gibson Assembly Kit (Cat # E2611S, New England Biolabs). Assembled constructs were confirmed by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 5-min extension cycle using Q5® High-Fidelity 2X Master Mix (New England Biolabs). PCR products were then purified using QIAquick® Gel Extraction Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... The unbound fraction was then incubated for 1 h at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... a Tn7-based GPS4 transprimer-mutagenized (NEB) etpA genes36 with insertions in the C-terminal repeat region of etpA were amplified from the indicated template plasmid (supplementary table 1 ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR with OneTaq 2X master mix (NEB) were used to verify loss of target gene and correct cassette integration86 ...
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bioRxiv - Molecular Biology 2024Quote: ... prior to expression as a GST-fusion in T7 Express cells (New England Biolabs). Recombinant human Sf3b1 was purified with a GS Trap (Cytiva) ...
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bioRxiv - Microbiology 2024Quote: The gp7 was cloned using HiFi assembly (New England Biolabs) of PCR-generated products with pETYSBLIC3c plasmid (53) ...
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bioRxiv - Microbiology 2024Quote: ... For Nanopore sequencing high-molecular weight DNA was isolated from a separately cultivated batch with the Monarch Genomic DNA Purification kit (New England Biolabs, Frankfurt, Germany) and library preparation was performed with 1.5 µg using the ligation sequencing kit 1D (SQK-NBD114.24 ...
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bioRxiv - Microbiology 2024Quote: ... These segments were cloned into BamHI-digested pLGB13 88 using NEBuilder (NEB). Plasmids were sequenced to confirm the correct assembly of the segments ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed in 25 μL reaction volumes using 12.5 μL of Taq 2X MasterMix (NEB), 9.5μL water ...
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bioRxiv - Microbiology 2024Quote: ... The library preparation was elaborated using the NEBNext® SingleCell/Low Input cDNA Synthesis kit (New England Biolabs). The prepared libraries were then sequenced ...
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bioRxiv - Microbiology 2024Quote: ... T4 Rnl2tr was inactivated by incubation with Proteinase K (0.04U/µL) (NEB, P8107) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the entire plate included 10 µl of OneTaq Quick-Load 2X Master Mix with standard buffer (New England Biolabs, MA, USA), 0.5 µl of each primer (10 µM) ...
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bioRxiv - Microbiology 2024Quote: ... An enzymatic cleanup was done to remove primer dimers and inactivate nucleotides by directly adding 0.5µL of Antarctic phosphatase and Exonuclease 1 (New England Biolabs, Inc; M0293L, M0289L) and 1.22µL Antarctic phosphatase buffer (1x final concentration ...
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bioRxiv - Microbiology 2024Quote: ... were digested with type II restriction enzymes (NEB), and the viral genome fragments were ligated with T4 ligase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant plasmids were created by PCR amplifying two (ops) or three (HP-ops) DNA segments using Phusion polymerase (NEB) with ΔmpiM44 as template with the primers listed in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with DpnI (New England Biolabs). All plasmids were transformed into E ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... followed by a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). For flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA was synthesized using the LunaScript™ RT SuperMix Kit (New England Biolabs) in 20 μL reaction containing 4 μL SuperMix (5X ...
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bioRxiv - Neuroscience 2024Quote: ... RT-PCR reactions were performed with Taq polymerase (New England Biolabs) with cDNA diluted to 10 ng/µL ...
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bioRxiv - Neuroscience 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...