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Citations for New England Biolabs :
5101 - 5150 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 cycles of pre-amplification were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) with indexed primers (Ad1 and Ad2) ...
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bioRxiv - Immunology 2022Quote: ... followed by a genomic PCR with Q5® High-Fidelity DNA Polymerase (#M0491S, New England BioLabs) kit ...
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bioRxiv - Developmental Biology 2022Quote: ... Transposed DNA was amplified with NEBNext High-Fidelity 2X PCR Master mix (New England Biolabs, M0541) as follows ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were purified using the Monarch PCR clean-up kit (New England Biolabs, MA, USA). The purified fragments and the pABC3-His or pABC3-GFP vector were digested with PacI and NotI restriction enzymes (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... SELEX libraries were amplified by PCR using the high-fidelity Phusion DNA polymerase (NEB cat. M0530L) and purified using the MinElute PCR Purification Kit (Qiagen cat ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR of the Hyl1La was done using Q5® High-Fidelity DNA Polymerase (New England Biolabs). The PCR products were run on the gel and the expected-sized PCR product was purified with a kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The 499 bp PCR product was cut with restriction enzyme Bsp1286I (New England BioLabs, Ipswich, MA). The mutant product was cut into 211 and 288 bp fragments ...
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bioRxiv - Plant Biology 2021Quote: ... The USER-PCR products was treated with the USER enzyme (New England Biolabs, Ipswich, MA, USA) to remove all uracil residue ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were amplified and recombined using the NEBuilder HiFi DNA assembly kit (New England Biolabs) followed by Sanger sequencing for identification of positive clones ...
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bioRxiv - Microbiology 2021Quote: ... the entire pGE450 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotide TGD4981 and TGD4982 ...
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bioRxiv - Microbiology 2021Quote: ... the entire pGE190 plasmid was amplified by inverse PCR using Q5 High Fidelity DNA polymerase (NEB) with oligonucleotides TGD4006 and TGD5162 ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product and pUAST-attB vector were digested with NotI and Kpn1 restriction enzymes (NEB) and ligated to obtain the desired clones ...
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bioRxiv - Plant Biology 2021Quote: ... or hopF1c (along with its chaperone shcF) were PCR-amplified using Q5 High fidelity polymerase (NEB) from Psa3 V-13 genomic DNA using cloning primers (Table S1 ...
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bioRxiv - Cell Biology 2021Quote: ... PCR confirmation of endogenously tagged ATR cell lines was performed using NEB Taq DNA polymerase (NEB) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were incubated overnight at 60 °C with either BstNI restriction enzyme (New England Biolabs) or a control buffer solution without enzyme ...
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bioRxiv - Genomics 2020Quote: ... Sheared DNA was purified using either the Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA) or QIAquick Nucleotide Removal Kit (QIAGEN) ...
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bioRxiv - Immunology 2020Quote: ... Each PCR reaction consisted of 50μL of NEBNext Ultra II Q5 Master Mix (NEB, Cat# M0544), 1μg of gDNA ...
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bioRxiv - Genetics 2022Quote: ... Putative successful amplicon clones were identified by PCR amplification using 2xOneTaq Master Mix (New England Biolabs) with primers DLO883 (5’-CAGGAAACAGCTATGACCATG-3’ ...
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bioRxiv - Genetics 2022Quote: ... the following was mixed: 20 µL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 µL 10 µM forward primer ...
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bioRxiv - Genetics 2022Quote: The unc-5 locus was amplified for sequencing by PCR using OneTaq 2x Master Mix (NEB) with primers DLO1081 (5’-TCTTTTCAGGCTTTGGCACTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... These PCR fragments contained flanking homology for assembly using a Gibson Assembly kit (New England Biolabs) to yield the final vector pMAD2-5’HA-gfpmut2-3’HA (pNW2410 ...
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bioRxiv - Cell Biology 2022Quote: ... two overlapping PCR products were generated using the high-fidelity DNA polymerase Q5 (New England Biolabs): one was the linearized recipient CD9 plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... PCR amplification of desired fragments was performed using NEB Q5 High-Fidelity DNA polymerase (#M0491S, NEB). The PCR fragments were assembled into the linearized vector (pRS305 ...
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bioRxiv - Developmental Biology 2022Quote: ... Up to 1000 ng of purified PCR product was used with the HighScribe T7 kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... The purified PCR product and pcDNA3.1-NefSF2-V5-VC were digested with EcoRI and NotI (NEB), and the DNA was isolated by column purification (Zymo Research) ...
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bioRxiv - Systems Biology 2019Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: The masA gene was amplified by PCR from JE2 using Phusion high-fidelity DNA polymerase (NEB) and primers MasFW ...
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bioRxiv - Genetics 2019Quote: ... Barcodes were added in a second round of PCR using Phusion DNA polymerase (NEB cat: M0531S). Samples were sequenced with 76 base-pair paired-end reads using an Illumina NextSeq (Illumina ...
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bioRxiv - Genomics 2019Quote: ... Then the DNA on beads was used for PCR amplification with phusion DNA polymerase (NEB, M0530) and purified with VAHTS DNA Clean Beads to select the DNA fragments between 200bp and 600bp ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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bioRxiv - Synthetic Biology 2020Quote: DNA amplification was done via PCR using Q5 Hot Start HiFi 2X Master Mix (NEB, M0494). All plasmids were constructed using either blunt end ligation or Gibson Assembly ...
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bioRxiv - Bioengineering 2019Quote: ... DNA fragments containing the AOX1 promoter and gene were amplified by PCR using Vent polymerase (NEB) with primer combinations AOX1P-F and AOX1P-R for the promoter and AOX1G-F and AOX1G-R for the gene (Table S3).
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bioRxiv - Synthetic Biology 2020Quote: ... Typically, pL0s were generated via PCR (Q5 and OneTaq hot-start polymerases, New England BioLabs (NEB)) followed by In-Fusion (Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified products were cloned using the NEB PCR cloning kit (New England Biolabs, Ipswich, MA). Clones from each founder were Sanger sequenced using the NEB analysis primers to define indels.
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bioRxiv - Synthetic Biology 2019Quote: ... Polymerase chain reactions (PCRs) were conducted using Q5 High-Fidelity 2x Master Mix (New England Biolabs) in the presence of 0.5 µM of forward and reverse primers in a volume of 25 µL ...
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bioRxiv - Genetics 2020Quote: ... The PCR-amplified Vluc and nonsense Gluc sequences were cloned into NcoI-(R0193S, New England BioLabs) and XbaI-(R0145S ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCRs was made using the Q5 High-Fidelity polymerase from New England Biolabs (NEB, M0492L).
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bioRxiv - Plant Biology 2020Quote: ... were PCR amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Cat n° M0491) with oligonucleotide primers containing attB recombination sequences ...
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bioRxiv - Developmental Biology 2020Quote: ... The bcd-3’UTR was PCR amplified using Q5 high-fidelity polymerase (New England Biolabs, M0491S) from genomic DNA using the primers 5’-GAGTCATCA-TCATCAGTTTCGTCAAAAGTAACCTGGATGAGAGGCGTGTTAGAG-3’ and 5’-CTGGGTCG-GCGCGCCCACCCTTGTCTAGGTAGTTAGTCACAATTTACCCGAGTAGAGTAG-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA fragments were PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (NEB) and oligonucleotides described in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB) and propagated following the instructions of CloneJET PCR Cloning Kit (Thermo) ...
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bioRxiv - Biochemistry 2020Quote: ... Both PCR steps followed the recommended temperatures and conditions using Q5 Master Mix (New England Biolabs). The PCR products from the first step were purified using 1% agarose gel ...
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bioRxiv - Synthetic Biology 2019Quote: All PCRs were performed using the Q5 Hot Start High-Fidelity Polymerase (New England Biolabs, NEB). PCR products were analysed on 1-2 % TAE or TBE agarose gels ...
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bioRxiv - Synthetic Biology 2019Quote: All PCRs were performed using the Q5 Hot Start High-Fidelity Polymerase (New England Biolabs, NEB). PCR products were analysed on 1-2 % TAE or TBE agarose gels ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting fragments were amplified for 14 cycles using NEBNext Ultra II PCR protocol (New England BioLabs) and quality was assessed with the Agilent High sensitivity DNA kit ...
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bioRxiv - Genomics 2019Quote: ... 20 ng of the forward backbone was amplified with Len_lib_linF and Len_lib_linR (Supplemental Table 8) using NEBNext High-Fidelity 2X PCR Master Mix (NEB); the minimal promoter and GFP was amplified from 10 ng of the pLS-mP plasmid using minGFP_Len_HAF and minGFP_Len_HAR (Supplemental Table 8) ...
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bioRxiv - Systems Biology 2021Quote: ... Two-hundred picomoles of the library was PCR amplified over 15 cycles with oligonucleotides Oligo_library_F and Oligo_library_R using Q5 polymerase (New England Biolabs). Amplified DNA was PCR purified (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed on the cDNA using high-fidelity DNA polymerase (New England Biolabs Phusion polymerase) using a forward primer targeting the start of rimA (PFLU0263 ...
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bioRxiv - Microbiology 2021Quote: ... Candidate genes or operons were PCR amplified using Q5 high fidelity DNA polymerase (New England Biolabs) according to manufacturer’s instructions using primers (No ...