Labshake search
Citations for New England Biolabs :
5101 - 5150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using NEB Monarch Total RNA miniprep kit (NEB cat. T2010S) following manufacturer’s manual with on-column DNase I digestion ...
-
bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
-
bioRxiv - Cell Biology 2024Quote: ... The obtained DNAs were used for Adaptor Ligation using adaptors and enzymes provided in NEBNext Multiplex Oligos for Illumina (NEB#E7335) and following the kit’s reaction conditions ...
-
bioRxiv - Cell Biology 2024Quote: ... via ligation-independent cloning with the NEBuilder® HiFi DNA Assembly Master Mix (NEB E2621S). Correct plasmid insert sequences were confirmed by Sanger sequencing (University of Utah DNA Sequencing Core ...
-
bioRxiv - Cell Biology 2024Quote: ... and reverse transcribed with M-MuLV Reverse Transcriptase (New England Biolabs, M0253) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... The PCR products were introduced in pMal-c2 (New England Biolabs) expression vector through FastCloning [34] ...
-
bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were then incubated with MBP amylose magnetic beads (New England Biolabs) for 1 h at 4°C and washed with 200 mM NaCl ...
-
bioRxiv - Cancer Biology 2024Quote: ... which was linearized with Esp3I enzyme (NEB #R0734L). The ligation was performed by adding an equal volume of NEB HiFi DNA Assembly Kit (#E5520S ...
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by NEBNext Ultra II DNA Library Preparation Kit protocol (NEB #E7645L) and analyzed by Agilent 4200 TapeStation ...
-
bioRxiv - Cancer Biology 2024Quote: ... and BamH1-HF (NEB, #R3136S) restriction enzymes followed by In-Fusion Snap Assembly (Takara ...
-
bioRxiv - Cancer Biology 2024Quote: ... Coli (NEB, #C3040H) and constructs were purified using the manufacture’s instruction of the MIDI-Prep system (Macherey-Nagel ...
-
bioRxiv - Cancer Biology 2024Quote: ... Assembly reaction was performed at 1:2 vector: insert ratio using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs #E5520) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... and templates were digested using Dnp1 (New England Biolabs #R0176). Assembly reaction was performed at 1:2 vector ...
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using Ultra II FS library preparation kit (New England Biolabs) and amplified with KAPA HiFi HotStart Real-time PCR using KAPA P5 and P7 primers (KAPA Biosystems).
-
bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and single-end (1 × 75 bp ...
-
bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
-
bioRxiv - Developmental Biology 2024Quote: ... mRNA was enriched using the New England Biolab’s (NEB) NEBNext Poly(A ...
-
bioRxiv - Developmental Biology 2024Quote: ... mRNA isolation module for samples with RINs greater than 7 and libraries were prepared using the NEBNext Ultra II directional RNA library preparation kit (NEB, E7760). QC was then performed on these libraries using an Agilent Bioanalyzer 2100 and the libraries were quantified using fluorometric methods ...
-
bioRxiv - Cell Biology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit (NEB) was used to delete the miniTurbo sequence from the above pLVX-KRT5-miniTurbo-FLAG-IRES-Puro and pLVX-KRT6A-miniTurbo-FLAG-IRES-Puro constructs ...
-
bioRxiv - Cell Biology 2024Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology ...
-
bioRxiv - Neuroscience 2024Quote: ... coli (C2987H, NEB) and Plasmid Miniprep Kit (27106 ...
-
bioRxiv - Molecular Biology 2024Quote: ... using NEB HiFi DNA Assembly Master Mix (NEB, #E2621), following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was conducted using NEBNext rRNA depletion kit v2 (NEB, #E7400L) followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research ...
-
bioRxiv - Molecular Biology 2024Quote: ... The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB, #E7300S). Pair-end sequencing reads of size 150 bp were produced for Ribo-seq on the Ilumina Hiseq X-ten system ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... sample was washed with 1x T4 ligase buffer (NEB) (2,500 xg ...
-
bioRxiv - Molecular Biology 2024Quote: ... to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB) (2,500 xg ...
-
bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was resuspended in buffer IV (160 µl annealed oligos, 1.2x T4 ligase buffer, 6,000 U T4 ligase, 1x protease inhibitors; NEB) to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... 850 U DpnII (NEB) and 35 µl rSAP (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 35 µl rSAP (NEB) were added and sample was incubated for at least 8 h at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... were annealed (81 µl of each Oligo 100 µM, 1x T4 ligase buffer; NEB) at 98 °C for 5 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Restriction free cloning was conducted using NEBuilder® HiFi DNA Assembly (NEB: E2621L). Isolation of plasmids and purification of PCR products were conducted by using commercially available kits from either Qiagen or Macherey-Nagel ...
-
bioRxiv - Cancer Biology 2024Quote: ... then split in 2 for PCR amplifications with either Minus or Plus primers using Q5 DNA Polymerase (New England Biolabs) with the following cycling conditions ...
-
bioRxiv - Cancer Biology 2024Quote: ... end-repair and A-Tailing were directly performed using the NEBNext UltraII DNA kit (New England Biolabs). A new custom universal Y-adapter based on the Illumina TruSeq sequences was designed to include a Unique Molecular Index (UMI ...
-
bioRxiv - Cancer Biology 2024Quote: ... and ligation (T4 DNA Ligase, NEB) and transformation (chemically competent NEB 5-alpha E ...
-
bioRxiv - Cancer Biology 2024Quote: ... fragments and destination plasmid were digested using the respective restriction enzymes (NEB) and ligation (T4 DNA Ligase ...
-
bioRxiv - Cancer Biology 2024Quote: ... Shipston and was N-terminally attached to an RFP (BKCa-DECRFP) using KpnI and BamHI restriction sites after PCR amplification (NEB Q5 High-Fidelity DNA-Polymerase, New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Cancer Biology 2024Quote: ... Shipston and was N-terminally attached to an RFP (BKCa-DECRFP) using KpnI and BamHI restriction sites after PCR amplification (NEB Q5 High-Fidelity DNA-Polymerase, New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Cancer Biology 2024Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5ng of cDNA were amplified with the long LongAmp Taq 2X Master Mix (New England Biolabs) for 25 cycles ...
-
bioRxiv - Cancer Biology 2024Quote: ... modified with an EF1a promoter and a Basticidin resistance gene using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Specific primers and NEBuilder HiFi DNA Assembly Master Mix were used to introduce the exon E6a or the R238W mutation into the canonical sequence of NT5C2.
-
bioRxiv - Biophysics 2024Quote: ... 2.2.7+ (New England Biolabs Inc., Ipswich, MA) and SnapGene v ...
-
bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
-
bioRxiv - Plant Biology 2024Quote: ... MpGDI2 (Mp6g05010) and MpNAC7 (Mp6g02620) by PCR using Phusion polymerase (NEB). Arabidopsis Bobber1 (At5g53400 ...
-
bioRxiv - Plant Biology 2024Quote: ... All obtained fragments were further incubated with dATP and Taq DNA polymerase (NEB) for 1 hour before cloned into the gateway compatible vector PCR8/GW/TOPO TA (Invitrogen ...
-
bioRxiv - Biophysics 2024Quote: ... into a pSGEM construct with five consecutive repeats of the myc tag (Lörinczi et al. 2015)) using NcoI and HindIII (New England Biolabs).
-
bioRxiv - Evolutionary Biology 2024Quote: ... Fully homogenized tissue was then used for DNA purification using the “Protocol for Extraction and Purification of Genomic DNA from Tissues” protocol for extraction and purification of genomic DNA from tissues in the Monarch® Genomic DNA Purification Kit (New England Biolabs T3010). DNA extract quality was assessed for quality (260:230 nm and 260:280 nm wavelengths ...
-
bioRxiv - Biochemistry 2024Quote: ... using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, Frankfurt/Main, Germany). Pdxp-D14N was generated with the Platinum SuperFi II DNA Polymerase Mastermix according to mutagenesis protocol A provided by the manufacturer (Thermo Fisher Scientific ...