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Citations for New England Biolabs :
5051 - 5100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Oligos were annealed and both oligos and vector were digested with AgeI and EcoRI (NEB). Lenti-sgRNA-neo (Addgene #104992 ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Second-strand cDNA was synthesized using Deep Vent (exo-) DNA polymerase (NEB) and biotinylated second-strand primer ...
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bioRxiv - Cancer Biology 2024Quote: ... purified with tRNA-coated streptavidin beads and amplified with 2x Phusion high-fidelity PCR mix (NEB). CAGE libraries were treated with Exonuclease I (NEB ...
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bioRxiv - Developmental Biology 2024Quote: Tissue samples were then digested with Proteinase K (NEB, P8107S) as described (38 ...
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bioRxiv - Cell Biology 2024Quote: ... lentivectors for CRISPR Knock-out (CRISPR-KO) experiments via restriction digest of the respective lentivector with BsmBI (NEB, Cat. No. R0739) and T4 ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and T4 ligase (NEB, Cat. No. M020L). Oligonucleotides (Eurofins Genomics ...
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bioRxiv - Biophysics 2024Quote: The TRPV1-exCellHalo construct was built using the standard Gibson Assembly method of cloning (NEB, MA). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA was ligated to the 3′ adaptor tRNA using T4 RNA ligase 2 (NEB, M0351L) for 2 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the genomic region (∼2000 bp) surrounding the natural start codon on exon 1 was cloned into the pUC19 vector (New England Biolabs, N3041S) by Gibson Assembly ...
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bioRxiv - Cancer Biology 2024Quote: ... 100µM gDNAs were phosphorylated and annealed with T4 PNK (NEB, M0201S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... For this, the pcDNA3 vector was linearized using BamHI (Neb, R3136S) and EcoRI (NEB, R3101T). CASP3 was amplified from pcDNA3-Casp3-myc (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... the pLenti CRISPR V2 was digested for 2 h at 50°C with Bsmb1 (NEB, R0580S) and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: ... 600 units of lambda phosphatase (NEB) was added to reactions together with 1 mM MnCl2 and incubated for 30 min at 30 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared from 20 uL of ChIP DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Library molarity and quality were assessed by Qubit and Tapestation (DNA High sensitivity chip) ...
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bioRxiv - Molecular Biology 2024Quote: ... m6A-RIP mRNA was purified using the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were eluted in 8 µl elution buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB, E6150S) and purified using Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs), profiled using the Agilent TapeStation D1000 high sensitivity ScreenTape on the Agilent 4150 TapeStation System ...
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bioRxiv - Neuroscience 2024Quote: ... we synthesized (EnGen, NEB E3322S) and purified (Zymo R1018 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pRL814 (containing green fluorescent protein (GFP) under control of PT7A1-O34) backbone was digested with NdeI and HindIII-HF (NEB). The digested backbone and amplified genes were ligated using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and amplified genes were ligated using NEBuilder HiFi DNA Assembly Master Mix (NEB) and transformed into chemically competent E ...
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bioRxiv - Microbiology 2024Quote: ... The resulting amplicon was then used as a template in a PCR reaction (Q5, NEB) to add ends compatible with sequencing using custom primers (Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... and the viral genome fragments were ligated with T4 ligase (NEB). The purified ligation product was used as the template for in vitro transcription to produce full-length viral genome using mMESSAGE mMACHINE T7 Ultra Kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... a recombination template composed of 500 bp upstream and downstream of the comEC gene was assembled using Gibson Assembly (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2024Quote: ... The extracted RNA was treated with DNase I (NEB) and purified with the Monarch RNA clean-up kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction was set up using Q5 polymerase (New England Biolabs, Whitby, Canada) following the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2024Quote: ... All fractions were tested for phage titre and the fractions containing phage were also tested for the presence of DNA by treating a sample of each fraction with MseI and HindII (New England Biolabs, Whitby, Canada) restriction enzymes for 15 min at 37°C according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Ultra II DNA Library prep Kit (E7370; NEB). Libraries were sequenced using an Illumina NextSeq 2000 sequencer 75 bp Single End.
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed by PCR with Illumina P5 and P7 primers using Q5 High-Fidelity DNA Polymerase (#M0491L, NEB), and purified with 1.5x AMPure XP beads ...
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bioRxiv - Microbiology 2024Quote: ... precipitated using an isopropyl alcohol wash and ethanol and then rehydrated in Elution Buffer (New England Biolabs, Whitby, Canada). DNA concentration was measured using a QuBit 4 Fluorometer and the QuBit dsDNA High Sensitivity Quantitation kit (Invitrogen ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... single colonies were picked and used as a template for amplification by PCR using Q5 DNA polymerase (New England Biolabs, Whitby, Canada) with primers CR1_F and R as well as CR3_F and R (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...
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bioRxiv - Microbiology 2024Quote: ... and purified with the Monarch RNA clean-up kit (NEB). cDNA was prepared from 1 μg of RNA ...
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bioRxiv - Microbiology 2024Quote: ... the PCR amplicons were purified used the Monarch PCR and DNA Cleanup Kit (New England Biolabs, Whitby, Canada) and sent for Sanger sequencing at the McMaster Genomics facility ...
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bioRxiv - Microbiology 2024Quote: Lysates were treated with 20 μg·ml-1 of RNase A and 2U·ml-1 of DNase I (New England Biolabs, Whitby, Canada) along with 10% DNase buffer 10x at 37°C for 30 min followed by heat inactivation at 75°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... coli strain NEB10β (New England Biolabs) and all inserts were verified by sequencing ...
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bioRxiv - Microbiology 2024Quote: ... HindIII-HF or NotI-HF (New England Biolabs, Whitby, Canada) and 22 μl of phage buffer ...
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products were cloned into the PmeI site of the pseudo-suicide allele-coupled exchange (ACE) vector pMSR0 38 using NEBuilder HiFi DNA Assembly (New England Biolabs). The resulting plasmids ...
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bioRxiv - Microbiology 2024Quote: ... precipitated using an isopropyl alcohol wash and ethanol and then rehydrated in Elution Buffer (New England Biolabs, Whitby, Canada).
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s recommendations (New England Biolabs, Whitby, Canada). The plasmid was then purified using the NEB Miniprep kit and then electroporated into electrocompetent S ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendations for DNA purification from gram-positive bacteria and Archaea ...
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bioRxiv - Microbiology 2024Quote: ... T4 DNA ligase (New England Biolabs), and restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: High molecular weight DNA for long-read sequencing was extracted using the Monarch HMW DNA extraction kit for tissue (NEB), with the specific protocol for Gram-negative bacteria ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were cloned in front of the luxCDABE operon in the plasmid pUC18R6KT-mini-Tn7-KmR-luxCDABE into the SpeI and XmaI restriction sites (highfidelity enzymes, NEB). Constructs were verified for mutations by sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were subsequently treated with 10 U of calf intestinal alkaline phosphatase (NEB, 10,000 U/ml) for 1 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Restriction digestion with the XhoI (New England Biolabs, Ipswich, MA) was performed according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2024Quote: ... cells from a transfected well were used for RNA extraction using a Monarch total RNA miniprep kit (NEB). Isolated RNA was subjected to denaturing gel electrophoresis alongside a molecular weight ladder (Dynamarker prestain marker for RNA high ...