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Citations for New England Biolabs :
5001 - 5050 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and PCR was performed with OneTaq Standard 5X buffer and OneTaq Polymerase (New England Biolabs) using a 96-well Thermal Cycler (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified and digested with 10U of lambda exonuclease (New England Biolabs; M0262) in 1X lambda-exonuclease buffer for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... was cloned downstream the tagBFP combining inverse PCR and blunt end ligation (T4 Ligase NEB). The sequence of circ-HDGFRP3 (exons 2-3-4-5 ...
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bioRxiv - Molecular Biology 2024Quote: ... the ends of the PCR fragments were then phosphorylated using T4 polynucleotide kinase (PNK, NEB) before undergoing ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were run on 1.5% agarose gel alongside 50-bp DNA ladders (NEB #N0556S). Image Lab software (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The raw PCR product was used for blunt-end ligated (NEB; 30mins at 37°C). The circularized plasmids were transformed into E ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR fragment and pSLQ1651-sgTelomere (F+E) were digested with NheI and EcoRI (NEB), ligated and transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB) for sequencing.
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bioRxiv - Microbiology 2024Quote: ... All PCR products were subsequently digested with the respective restriction enzyme pair (New England Biolabs), gel-purified ...
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bioRxiv - Microbiology 2024Quote: ... The resulting amplicon was then used as a template in a PCR reaction (Q5, NEB) to add ends compatible with sequencing using custom primers (Table 2 ...
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bioRxiv - Microbiology 2020Quote: ... pET20b::bepA::6xHis vector was used in 18 cycles of PCR using the Phusion polymerase (NEB) as described by the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... PCR reactions were prepared using Q5 High-Fidelity 2X Master Mix (New England BioLabs, Ipswich, MA) and 10 ng genomic DNA in a final volume of 25 μl ...
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bioRxiv - Cell Biology 2020Quote: ... by individual mutagenic PCR reactions combined with NEBuilder® HiFi Assembly (New England Biolabs, Ipswich, MA). Plasmid pSM3393 was the template for all reactions ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were run on 1.5% agarose gel with 100bp ladder (New England Biolabs, Cambridge, MA) and visualized on an iBright digital gel imager (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Each sample was amplified separately by two PCR reactions using Q5 high-fidelity polymerase (NEB, M0149S) and zone-specific PCR primers ...
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bioRxiv - Genomics 2020Quote: ... The L2:DDRGK1 sequence was PCR amplified with Phusion High Fidelity DNA polymerase master mix (NEB), using cDNA generated in the L2:DDRGK1 CRISPRa experiment with gRNA 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments were PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (NEB) and oligonucleotides described in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: The resulting PCR fragments were gel purified using the Monarch Gel Purification Kit (New England Biolabs) and TA cloned into pGEM-T Easy (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The tln2ai23 allele was genotyped by PCR followed by SrfI restriction digest (New England Biolabs, R0629L). The primers used for PCR were ...
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bioRxiv - Developmental Biology 2021Quote: ... 10ul of PCR product was digested with 5 units of Msel (New England Biolabs, Ipswich, Massachusetts) for one hour ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs). PCR product was run on an agarose gel and genotype was determined on size (168bp ...
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bioRxiv - Developmental Biology 2022Quote: ... 5µl of the PCR mix was digested overnight with 5 units of BbsI (New England Biolabs) in 30 µl volume ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB Cat. #: M0492) for 20 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat.# E2621S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments used for plasmid construction were amplified by Q5 DNA polymerase (M0491, New England Biolabs) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of PCR product containing barcoded oligos were inserted into 1 μg SfiI (NEB, R0123) digested empty vector A or P by gibson assembly NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were gel purified with Monarch® DNA Gel Extraction Kit (T1020S, New England BioLabs) according to manufacturer’s instructions and sent for NGS at the Gurdon Institute NGS core using an Illumina HiSeq 1500.
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bioRxiv - Genomics 2020Quote: ... PCR amplification (8-9 cycles) was performed with 10 μL Fusion HF Buffer (New England Biolabs), 3.125 μL 10uM TruSeq Primer 1 ...
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bioRxiv - Genomics 2020Quote: ... which yielded an approximately 1kb PCR amplicon with a Phusion High Fidelity Polymerase (New England Biolabs) and the following PCR protocol ...
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bioRxiv - Genomics 2020Quote: ... The purified ligated DNA was subject to PCR amplification with Q5 high-fidelity DNA polymerase (NEB), followed by purification with Agencourt AMPure XP (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... mutation of SSL2 (ssl2*) was accomplished by standard PCR reactions using Taq polymerase (New England Biolabs). ssl2* PCR products were then transformed into yeast along with a linearized pRS315 SSL2 LEU2 plasmid with most of the wild-type SSL2 sequence removed by restriction digest ...
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bioRxiv - Microbiology 2019Quote: ... and PCR enrichment (8 cycles) with NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA). DNA concentrations were estimated using qPCR and the Kapa Library Quant Kit (Kapa Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... and genomic loci were amplified by PCR utilizing the Phusion Hot Start Flex DNA Polymerase (NEB). Amplicons were enzymatically purified and submitted for Sanger sequencing (Genewiz) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Both the PCR product and plasmid were digested by EcoRI HF and KasI (New England BioLabs) and purified ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were then individually ligated using a KLD Enzyme reaction kit (New England Biolabs) for 3 hours and subsequently transformed into ElectroMAX DH10B E ...
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bioRxiv - Microbiology 2019Quote: ... pET20b::bepA::6xHis vector was used in 18 cycles of PCR utilising the Phusion polymerase (NEB) as described by the manufacturer ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Microbiology 2019Quote: ... All PCRs were performed using the high-fidelity polymerase Q5 (New England Biolabs [NEB], Ipswich, MA) and the primers listed in Table S3 ...
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bioRxiv - Microbiology 2019Quote: ... All PCRs were performed using the high-fidelity polymerase Q5 (New England Biolabs [NEB], Ipswich, MA) and the primers listed in Table S3 ...
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bioRxiv - Molecular Biology 2019Quote: ... Associated DNA was then purified by extraction using Monarch PCR & DNA Cleanup Kit (New England Biolabs). Immunoprecipitated DNA and total input were used as a template for real-time qPCR ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR procedures followed the protocol for using Q5 high fidelity DNA polymerase (NEB, Ipswich, MA). The full length SpAxin cDNA served as a template to make single domain deletions of Axin to remove the conserved APC ...
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bioRxiv - Microbiology 2019Quote: ... The PCR amplification was performed using the Q5® High Fidelity polymerase (New England Biolabs, USA) and the final reaction contained lx Q5® reaction buffer ...
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bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... PCRs were performed on cDNA using the Phusion® High-Fidelity DNA Polymerase (New England BioLabs) and appropriate primer pairs ...
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bioRxiv - Cancer Biology 2019Quote: ... After determining accurate concentrations by quantitative PCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 15-20% PhiX spike-in ...