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Citations for New England Biolabs :
5051 - 5100 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The insert was synthesized by IDT and amplified via PCR using Phusion high GC buffer (NEB) supplemented with 3% dimethylsulfoxide (DMSO ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were generated by Gibson assembly of PCR fragments using the NEBuilder HiFi Master Mix (NEB) or in vivo assembly (31) ...
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bioRxiv - Genomics 2019Quote: ... Libraries were amplified for 12 cycles using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) in total volume of 25µl in the presence of 800nM of barcoded primers (400nM each ...
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bioRxiv - Genomics 2019Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1_noMX universal primer ...
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bioRxiv - Synthetic Biology 2019Quote: ... Polymerase chain reactions (PCRs) were carried out with Q5 Hot Start High-Fidelity DNA polymerase (NEB). Colony PCRs were performed with Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 µl of PCR product was then mixed with 1.5 µl 10X NEBuffer 2 (B7002S, NEB) and 1.5 µl of Nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... smaller DNA fragments (<100 bp) were purified using the Monarch PCR Purification Kit (New England Biolabs) with a modified protocol ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were engineered for digestion using Kpn1 and Hind3 restriction enzymes (New England Biolabs) and inserted into the pGL4.23 vector (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Circularized ssDNA was next purified and concentrated using a Monarch® PCR & DNA Cleanup Kit (NEB). 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: PCR amplicons corresponding to the enhancer segments were generated using Q5 polymerase (New England Biolabs M0492S), except for segment 2 which was generated with Kapa polymerase supplemented with 1 M Betaine ...
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bioRxiv - Synthetic Biology 2021Quote: ... Genomic PCR was performed using Q5® High-Fidelity 2X Master Mix (New England Biolabs, M0492L) according to the manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA templates for MS-QBiC peptide synthesis were amplified by PCR using Taq DNA polymerase (NEB) with a T7 promoter primer (5′-GGGCCTAATACGACTCACTATAG-3′ ...
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bioRxiv - Systems Biology 2020Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic sequence of isolated clones at targeting locus were further confirmed by PCR cloning (E1202S, NEB) and Sanger sequencing (AGRF ...
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bioRxiv - Biochemistry 2020Quote: ... All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB, M0493S). Reagents for the KLD reactions came from NEB ...
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bioRxiv - Biochemistry 2020Quote: RNA was transcribed from PCR-amplified inserts using the Hiscribe T7 high yield synthesis kit (NEB). 0.1µg/µl of the RNA of interest was heated to 90°Cfor 2 mins and cooled at 4°Cfor 2 mins before adding chilled 10X RNA structure buffer (500mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR amplifications were conducted using Taq DNA Polymerase with Standard Taq buffer (New England Biolabs). The nucleotide sequences for RT-PCR and real-time PCR primers (synthesized by Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... The cloning PCR reactions were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs # M0491L), and the cycling parameters were as follows for all cloning ...
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bioRxiv - Biochemistry 2021Quote: ... Table 3) region of clpP1 was amplified by PCR (GC buffer, Phusion polymerase, New England Biolabs) using isolated L ...
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bioRxiv - Biochemistry 2020Quote: ... The final PCR product was subcloned into pMiniT 2.0 vector (New England BioLabs, Whitby ON, Canada) and fully sequenced ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reaction contained: 0.4 units of Phusion High-Fidelity DNA Polymerase (New England Biolabs, M0530S), 13.4 μL ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs, M3003S) on an Applied Biosystems StepOnePlus™ Real-Time PCR system with the following conditions ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 μL of PCR product was produced using Phusion Hotstart Flex polymerase (New England Biolabs, M0535S). The entirety of this PCR product was run on a gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer were purchased through New England Biolabs (NEB). ssDNA substrates used for extension reactions were ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs, MA, USA), RNA PCR Primer1 (RP1) ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and performed 6–9 cycles of PCR with the NEBNext Ultra II Q5 Master Mix (NEB) using Illumina P7 and the Seq-Well P5-TSO hybrid primer (Gierahn et al. ...
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bioRxiv - Systems Biology 2020Quote: ... The Illumina adapters and the indices were added by a second PCR using Q5 polymerase (NEB). Each 50 μL PCR consisted of 2-10 μL of purified PCR from the first PCR ...
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bioRxiv - Plant Biology 2021Quote: ... The construct pNOC-ARS-CRISPR-compact and the PCR product was digested with NheI/MfeI (NEB) and ligated to generate the vector pNOC-ARS-CRISPR-P2A-BlastR (Supplemental Dataset S2).
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bioRxiv - Neuroscience 2020Quote: ... Genomic PCR was carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs, M0492L).
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bioRxiv - Molecular Biology 2021Quote: ... The petSUMO_Nsp1_WT construct was then obtained using PCR-products with Gibson Assembly® Master Mix (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Site directed mutagenesis was performed using the Phusion High-Fidelity PCR master Mix (New England Biolabs). All mutations were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2019Quote: ... The PCR reaction mixture contained 25 µL of 2X Taq polymerase master mix (New England Biolabs, MA ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative PCR was performed on cDNA with Luna Universal qPCR Mastermix (New England Biolabs) in a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again with a GeneRead Size Selection Kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... In case of any potential overlap we treated our PCR reactions with 1 µL DpnI (NEB) to eliminate the donor plasmid ...
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bioRxiv - Microbiology 2019Quote: ... and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were digested with restriction enzymes Bam H1 and Xba I (New England BioLabs, MA) or Bam HI and Xho I (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... was enriched by polymerase chain reaction (PCR) using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The amplified DNA samples were purified again by GeneRead Size Selection Kit (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... A 478 bp fragment of biotin labelled DNA was produced by PCR with Taq Polymerase (NEB) of λ-DNA using the following primers ...
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bioRxiv - Cell Biology 2021Quote: PCR products were gel purified and A-tailed by incubation with dATP and Taq Polymerase (NEB) for 30 minutes and ligated into pGEM-T-Easy (Promega).
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bioRxiv - Bioengineering 2022Quote: ... Samples were stored at -20° C before PCR amplification of cDNA with Q5 (New England Biolabs) or SuperFiII (ThermoFischer).
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bioRxiv - Microbiology 2022Quote: PCR was performed using colonies from transfer assays using OneTaq (New England BioLabs Inc., Ipswich, MA). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The polymerase chain reaction (PCR) was performed with “Taq DNA Polymerase with Standard Taq Buffer” (NEB) according to company’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 2.5 kilo base pair DNA tethers were PCR-amplified from the pUC19 plasmid (New England Biolabs) with a 5’-biotinylated primer on one side and a 5’-phosphoprimer on the other side ...
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bioRxiv - Genetics 2022Quote: ... Both PCR products were used for constructing the final vector with Gibson assembly Master Mix (NEB), according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... The substrate was obtained by PCR on CML cells derived gDNA using Q5 DNA polymerase (NEB) and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: The sequencing libraries were generated by PCR using Phusion DNA polymerase (NEB M0530S, or Thermo F530L). The 50ul PCR reaction was assembled by mixing 10ul of 5xHF buffer ...
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bioRxiv - Microbiology 2022Quote: ... All PCR amplified segments were assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB E2621) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... sgRNAs were synthesized in vitro from purified PCR products by using the SP6 RNA-polymerase (NEB). 20 μL reactions contained 1 μg DNA template ...