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Citations for New England Biolabs :
4901 - 4950 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Immunology 2024Quote: ... and PCR products were assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) to generate the final construct pET788 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reaction mix was purified with Monarch® PCR & DNA Cleanup Kit (NEB, Ipswich, MA). Next ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed with Phusion or Q5 High Fidelity DNA Polymerase (New England Biolabs). Primer sequences are provided in Supplementary Table 4.
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bioRxiv - Genetics 2024Quote: The PCR reaction contents were mixed with 6x DNA loading dye (New England Biolabs, B7024S) and separated on 1% agarose gel prepared in Tris-acetate-EDTA buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix was prepared for using the Phusion® High-Fidelity DNA Polymerase (NEB) and primer sequences are listed in Table S3 ...
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bioRxiv - Genomics 2024Quote: ... a SYBR green I based qRT-PCR kit (Luna Universal One-Step NEB, Ipswich, USA) was used in PCR mixtures (20 µl ...
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bioRxiv - Evolutionary Biology 2024Quote: ... extracted DNA was amplified using a two-stage Phusion High-fidelity DNA polymerase PCR (NEB). In a three-cycle PCR ...
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bioRxiv - Immunology 2024Quote: ... The reaction consisted of 50 µL of NEBNext High Fidelity 2X PCR Master Mix (NEB), 4 µg of genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... Linear HDR templates for knockout were PCR amplified using Phusion DNA polymerase (New England Biolabs) with T7 Forward and M13 Reverse primers ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... PCRs were carried out using the Q5 Hot Start 2x Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol for 30 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... The resultant PCR product was reassembled into a tdTomato-containing vector via Gibson assembly (NEB) with a tdTomato insert (sequence ordered as a codon-optimized gBlock from IDT ...
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bioRxiv - Biochemistry 2023Quote: ... plates that contain unc-104(R551H) allele was selected by genomic PCR and BamHI (NEB) digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR reactions were prepared using the Luna® Universal qPCR Master Mix (NEB). Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... The editing region was amplified with PCR using Q5 High-Fidelity 2X Master Mix (NEB) followed by purification the PCR product using the QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was mixed with the vector fragment and assembled with HiFi assembly (NEB). This strategy maintains variant barcode matching from residues for the library up until L290 ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted cDNA (100-fold) was used for two multiplex PCR reactions with Q5 polymerase (NEB) with two primer pools (Table S5) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colony PCR was performed using Quick-Load Taq 2X master mix (New England Biolabs, M0271L). Successful colonies were grown in 100 μg/mL ampicillin-rich lysogeny broth (LB ...
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bioRxiv - Microbiology 2023Quote: ... PCR was carried out with the Q5 DNA polymerase (New England Biolabs, USA, NEB #M0491). Primers were obtained from Eurofins Genomics (Germany ...
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bioRxiv - Microbiology 2023Quote: ... PCR was carried out with the Q5 DNA polymerase (New England Biolabs, USA, NEB #M0491). Primers were obtained from Eurofins Genomics (Germany ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis was performed using Phusion DNA polymerase PCR mix (New England Biolabs, UK). Primers were designed specifically to replace the nucleotides coding for serine at the 44th position in the CDS of OsbZIP1.1 with those for alanine as given in table S1.
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bioRxiv - Synthetic Biology 2023Quote: ... The PCRs were performed with Q5® High-Fidelity 2X Master Mix (New England Biolabs) with the designed synthetic primers (SigmaAldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Each pooled library was PCR amplified using Phusion polymerase (F-530) (New England Biolabs, M0530S). Quality of the libraries was assessed with a Bioanalyzer using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... All PCR products were gel purified by using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 μL nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction mixture contained: 12.5 µL LUNA LongAmp Taq 2x Master Mix (NEB M0287S), 5.5 µL Nuclease-Free Water (ThermoFisher Scientific #AM9937) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were obtained by PCR amplification for 15 cycles using Phusion DNA polymerase (NEB). After quantification with a micro fluorometer (TBS-380 ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments and linearized pDIA6754 (25) were mixed and assembled using Gibson Assembly (NEB, France) and transformed by heat shock in E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were driven by the Phusion High Fidelity DNA polymerase (New England Biolabs (NEB), M0530) ...
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bioRxiv - Molecular Biology 2023Quote: ... and (v) the four PCR fragments listed above were digested with DpnI (New England Biolabs), gel-purified ...
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bioRxiv - Genomics 2023Quote: ... All PCRs were run using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: Human ARHGAP18 constructs were created using Polymerase Chain Reaction (PCR) using New England Biolabs (NEB) Phusion High-Fidelity PCR Kit (catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... ATAC-seq libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541), a uniquely barcoded primer per sample ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Phyper-spank device and the pTRKH2 were PCR-amplified using the Q5 polymerase (NEB) and cloned by Gibson assembly25 in E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was concentrated using the Monarch PCR and DNA Cleanup kit (New England Biolabs, MA) with warmed elution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Truncated ric-8 constructs were generated by PCR using high-fidelity Phusion DNA polymerase (NEB). Phosphorylation mutants were created using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification of the libraries was performed using NEBnext Q5 Ultra II master mix (NEB) and the last sample cleanup was performed by double-sided PEG-magnetic bead purification to eliminate PCR primers and fragments above 1 kb in size ...
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bioRxiv - Bioengineering 2023Quote: ... All PCR reactions were performed using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L). Digestions were performed at 37C for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... size selection and PCR enrichment using the NEBNext Ultra Directional RNA Library Prep Kit (NEB). PCR enrichment step was performed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Integrated sgRNA sequences were PCR amplified with Q5 high-fidelity DNA polymerase (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed with 2x OneTaq HotStart DNA Polymerase master mix (New England Biolabs), using 6 μl of forward and reverse primer (1μM each) ...
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bioRxiv - Genetics 2023Quote: ... two consecutive PCRs were performed by using the Q5 High-Fidelity DNA Polymerase (NEB, M0491). For the PCR 1 ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification was performed with Q5 polymerase according to the manufacturer’s instructions (New England BioLabs) and the following thermal cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: Each PCR consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Four fragments were amplified by PCR using the Q5 High-Fidelity DNA Polymerase (NEB #M0492) and purified using Monarch PCR & DNA Cleanup kit (NEB #T1030) ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was purified by the Monarch PCR & DNA Cleanup Kit (New England Biolabs) and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2 ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids encoding for GAF2 mutants were prepared by Q5 site-directed PCR mutagenesis (NEB) of pET28a-GAF2 using the primers listed in Table S1.