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Citations for New England Biolabs :
4951 - 5000 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping PCR on purified genomic DNA was performed with Phusion High-Fidelity DNA Polymerase (NEB). PCR products were checked by agarose gel for single band amplification of the appropriate size and then subjected to Sanger sequencing for definitive identification of the 8 bp ch200 DNA deletion in ythdf2 exon 4 ...
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bioRxiv - Microbiology 2023Quote: ... and PCR carried out using Q5® High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: All cloning PCR were done using Q5 High-Fidelity DNA Polymerase (New England Biolabs, #M0491S) and InFusion HD cloning according to manufacturer’s protocol (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... Full length or truncated DAAM1 constructs were PCR-amplified using Phusion High Fidelity Polymerase (NEB) and cloned into pCDNA4/TO or pCS2 expression vectors ...
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bioRxiv - Biophysics 2022Quote: ... ∼210 bp regions nearby the target protospacers were amplified via PCR with Q5 polymerase (NEB) and primers containing Illumina adaptor sequences ...
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bioRxiv - Cell Biology 2023Quote: ... Purified PCR products and pAcGFP1-N1 were incubated with XhoI and BamHI (New England Biolabs) under 37 °C for 4 hrs in 1X buffer 3 supplemented with 1% BSA ...
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bioRxiv - Genetics 2023Quote: ... PCR products were visualized on 1% agarose gels with a 1 kb plus ladder (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... The PCR was done by using Q5 High-fidelity DNA Polymerase (New England Biolabs, M0491) in a mix carrying 10 μl Q5 PCR buffer (5x) ...
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bioRxiv - Developmental Biology 2023Quote: ... Homology arms (PCR products from genomic DNA) were inserted with Gibson assembly (New England Biolabs) into pDD287 (Addgene #70685) ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Library amplification PCRs were performed using NEBNext Ultra II Q5 Master Mix (New England Biolabs) on all the extracted genomic DNA to attach Illumina sequencing adaptors and barcodes ...
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bioRxiv - Genetics 2023Quote: DNA amplification was performed by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Subsequently the PCR samples (10µl) were digested with DPNI (0.2µl New England BioLabs GmbH, R0176S) in CutSmart Buffer to remove the wild-type background ...
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bioRxiv - Cell Biology 2023Quote: PCR reactions for cloning were performed using Q5 High-Fidelity DNA polymerase (New England Biolabs), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... DNA was purified using NEB Monarch PCR & DNA cleanup kit (New England Biolabs, catalog #T1030) according to the manufacturer’s instructions and eluted in 13µl H2O ...
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bioRxiv - Biochemistry 2023Quote: ... Libraries were generated from two PCR reactions both using Q5 High-Fidelity DNA Polymerase (NEB). Primers flanking mutated regions amplified ...
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bioRxiv - Synthetic Biology 2023Quote: ... All in vitro PCR reactions were performed using Q5 High-Fidelity 2X Master Mix (NEB). Yeast colony PCR reactions were done as previously reported20 using DreamTaq DNA polymerase (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR fragments used in the Gibson Assembly were amplified using Phusion HighFidelity DNA Polymerase (NEB) and were visualized on a blue LED transilluminator (https://iorodeo.com ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons (approximately 3.4 kb) were gel purified using Monarch DNA gel extraction kit (NEB). Library construction ...
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bioRxiv - Synthetic Biology 2024Quote: ... The tDNA was generated in a subsequent PCR reaction (Q5 High-Fidelity DNA Polymerase, NEB) and purified using the E.Z.N.A Cycle Pure Kit (Omega Bio-Tek) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Primers were purchased from Sigma-Aldrich in cartridge purified quality and used in PCR reactions with the Q5 high fidelity (NEB) or the Platinum SuperFi II polymerase (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR protocol involved two amplification steps using New England Biolabs (NEB, Ipswich, MA, USA) reagents ...
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bioRxiv - Genomics 2023Quote: ... The oligo library was amplified by four cycles of PCR using New England Biolabs (NEB) Phusion High-fidelity Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Conditional gene deletion was assessed by PCR amplification of genomic DNA using Taq polymerase (NEB) and oligonucleotides SMD357/8 flanking the TbGT8 locus ...
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bioRxiv - Neuroscience 2023Quote: ... occurred alongside library amplification with NEBNext High-Fidelity PCR Master Mix (New England Biolabs, M0541S). The PCR reaction was carried out with the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Twenty-five cycles of PCR were then performed using Q5 high fidelity DNA polymerase (NEB) and Illumina forward and reverse adapter primers (98°C for 10 s ...
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bioRxiv - Biophysics 2023Quote: ... and Q5 Hot Start High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, MA). The transcribed region had the following spacings ...
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bioRxiv - Biophysics 2023Quote: ... PCR was used to amplify templates for in vitro transcription with T7 RNA polymerase (NEB). The RNA product was extracted with phenol and concentration was measured by Nanodrop spectrophotometer (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent PCR product and pEF.CMV.RFP was digested with EcoRV according to the manufacturer’s protocol (NEB). These were then ligated together using T4 ligase (NEB ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... The chloramphenicol resistance cassette was amplified by PCR with Q5 High Fidelity DNA Polymerase (NEB), using primers that contained at least 50-bp of homology to the disrupted locus ...
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bioRxiv - Cell Biology 2023Quote: ... APOBEC converted DNA was PCR amplified using 22x cycles with Q5U master mix (NEB M0597S) with primer pairs cTF378+cTF381 for the promoter and oSA021+oSA023 for the gene body ...
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bioRxiv - Cancer Biology 2023Quote: ... DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... two rounds of PCR were conducted using the high fidelity Q5 Polymerase (New England Biolabs). The first round of PCR amplified the ‘naked’ coding region of the gene (*_110 and *_111 primers) ...
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bioRxiv - Molecular Biology 2023Quote: ... A single round of PCR was then performed using Q5 high fidelity DNA polymerase (NEB) with exon-specific primers appended with Illumina forward adapter sequence ...
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bioRxiv - Cell Biology 2023Quote: ... plates that contain unc-104(Q94L) allele was selected by genomic PCR and BamHI (NEB) digestion ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was mixed with the vector fragment and assembled with HiFi assembly (NEB). This strategy maintains variant barcode matching from residues for the library up until L290 ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... PCR on cDNA was performed with Q5 polymerase according to the manufacturer’s protocol (Biolabs, M0493S). The PCR product was digested with an enzyme that gains or loses a restriction site due to the specific P/LP variant in that fragment (Table 1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified with enzymes ExoI and rSAP (New England Biolabs, Ipswich, MA, USA), Sanger sequenced (Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... the pUC19-PRha-YFP-3×FLAG plasmid was inverse amplified by PCR (Q5 polymerase, NEB), omitting the yfp gene ...
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bioRxiv - Microbiology 2024Quote: ... Clean deletions were verified via PCR using Quick-Load Taq Master Mix (New England Biolabs) or Q5 High Fidelity Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR reactions were conducted using the Luna Universal qPCR Master Mix (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions were performed using Q5 High-Fidelity 2X Master Mix (NEB, Frankfurt, Germany). The PCR product was purified and sequencing was done using a gene specific primer (GSP3) ...
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bioRxiv - Genomics 2024Quote: ... cloning was performed using PCR of desired insert fragments with Q5 (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... Components of the insert were PCR amplified using Q5-Hot Start DNA Polymerase (NEB M0493) per manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB) with NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were generated by PCR with NEBNext Ultra II Q5 Master Mix (NEB M0544) in two consecutive reactions ...
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bioRxiv - Genomics 2024Quote: ... cloning was performed using PCR of desired insert fragments with Q5 (New England Biolabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB, # T1020), quantitated and next generation sequencing was performed using an Illumina NextSeq 75 cycle High Output kit on an Illumina NextSeq platform.
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bioRxiv - Microbiology 2024Quote: ... PCR amplifications were carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for cloning and DreamTaq™ DNA Polymerase (Thermo Fisher ...