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Citations for GE Life Sciences :
2901 - 2950 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... an equal amount of protein sample (between 10-20 μg) was separated by SDS-PAGE and then transferred onto a nitrocellulose membrane (Hybond, GE Healthcare) for two hours at 250 mA on ice ...
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bioRxiv - Cell Biology 2020Quote: ... The individual TMT labeled samples were pooled and then fractionated using strong cation exchange chromatography on an AKTA Pure 10 (GE Healthcare) equipped with a Luna 5 μm 100 angstrom 150 x 2.1 mm strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tag was cleaved with Prescission protease overnight and removed by molecular sieving using an analytical Superdex 75 10/300 chromatography column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer A (devoid of ATP and PefaBloc but containing 200 mM instead of 30 mM imidazole) and further purified over a Superdex 200 10/300 GL column (GE Healthcare) in buffer B (25 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: 10 μM purified WIPI2d was mixed with 20 μM of GST or GST-ATG16L1(207-230) and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 25mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 100 µL MkeTIR-SAVED (at least 100 µM) were injected into a size exclusion column (Superose 6 Increase 10/300 GL or Superose 12, GE Healthcare) equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... the flow-through containing Fab fragments was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... by inoculating cre+-Raptorfl/fl mice with 10 mg/kg TLR3 agonist poly(I:C) once every two days as indicated (GE Healthcare, WI) to induce Raptor cKO in the mouse ...
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bioRxiv - Biochemistry 2022Quote: ... of GlOCPX or SynOCP1 samples (5 mg/ml in 50 μl) was performed on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a Prostar 335 detector (Varian ...
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bioRxiv - Molecular Biology 2022Quote: ... to approximately 3 – 4 ml and further purified by size-exclusion chromatography at 18 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare). His-TEV-CobB ...
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bioRxiv - Biochemistry 2022Quote: ... The flow-through was concentrated to ∼3mg/mL (100kDa MWCO) and subjected to size exclusion chromatography on Superdex 200 increase 10/300 column (GE healthcare) in size exclusion buffer containing 50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration was adjusted to 250 mM KCl and the protein was loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... and the excess of dye was removed by using a PD Minitrap G-25 column for NPM or a Superdex 200 10/300 GL (GE Healthcare) size exclusion chromatography column for SET/TAF-Iβ ...
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... Gel filtration chromatography was performed as described in (Iwai et al., 2015) using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: Size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) was carried out using a Superdex 200 Increase 10/300 column (GE Healthcare) and a combination of a UV-Vis Prostar 335 (Varian ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
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bioRxiv - Immunology 2022Quote: ... FoxP2ΔN and His6-GST fused Runx1 (residues 371-451) were purified by a combination of chromatography on Ni-NTA agarose and Superdex 200 Increase 10/300 (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by size-exclusion chromatography on Superdex 75 Increase 10/300 (GE Healthcare) column ...
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bioRxiv - Microbiology 2022Quote: ... or His-NTV-AviTag (80 µl at 2 mg/ml) were injected on a Superdex 200 increase 10/300 GL column (GE Healthcare), equilibrated at 4°C with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and injected onto a Superdex200 10/300 GL column (GE Healthcare) equilibrated in the buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The final protein purification step and determination of oligomeric state was done on a Superose6 Increase 10/300 size exclusion column (GE Healthcare). The column was equilibrated in buffer B and the concentrated protein was loaded onto the column ...
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bioRxiv - Immunology 2022Quote: ... and an iterative algorithm was used to deconvolve the images using an optical transfer function of 10 iterations (Softworx, Applied Precision).
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Bioengineering 2022Quote: Ultrasound images of the incubated arteries were obtained with a standard clinical vascular ultrasound machine with a 10 MHz linear array transducer (LOGIQ V2, GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing pooled fractions from IEX were then loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) and collected in gel filtration buffer (20mM PIPES pH6.8 ...
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bioRxiv - Biophysics 2022Quote: ... Samples were eluted using buffer A containing 300 mM imidazole and were subsequently applied to a MonoQ 10/100GL column (GE Healthcare) and eluted using a linear NaCl gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and then purified from excess salt and potential oligomeric species using gel filtration on a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) at a flow rate of 0.5 mL/min and eluted in 20 mM sodium phosphate 0.2 mM EDTA buffer (at pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted fraction was concentrated and further purified by size exclusion chromatography using an S200 increase (10/300 GL) column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Cell Biology 2022Quote: ... 500 µg of the purified protein or the complex was loaded on a Superdex 200 10/300 size exclusion column (GE Healthcare) in line with both a DAWN HELIOS II MALS detector (Wyatt Technology ...
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bioRxiv - Biochemistry 2022Quote: ... the sample was also concentrated and subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble high-molecular weight seeding-competent tau (HMW SEC tau) was isolated from homogenate using size exclusion chromatography on a Superdex200 10/300GL column (#17-5175-01, GE Healthcare) as described previously(Takeda et al. ...
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bioRxiv - Plant Biology 2021Quote: ... PsbU and PsbV were removed by applying active dimeric PSII onto a size exclusion column (Superdex 75 10/300 GL, GE Healthcare) pre-equilibrated in CaCl2 buffer (1 M CaCl2 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Monomeric APNs were further purified by size-exclusion chromatography (SEC) on a Superdex 200 column 10/300 GL (GE Life Sciences) previously equilibrated in 25 mM Tris pH 8.0 and 150 mM NaCl.
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bioRxiv - Microbiology 2020Quote: ... concentrated and applied onto a size exclusion chromatography (SEC) column (SD200 increase 10/300 or SD200 16/600 pg, GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed thrice in PBS and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 µm; GE Healthcare). All staining steps were performed at room temperature for one hour ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... a pool containing Staphopain A was directly loaded on a HiPrep CM Fast Flow 16/10 (#28-9365-42, GE Healthcare) column equilibrated with NaAc5.5 and the column was washed until OD280nm reached a baseline ...