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Citations for GE Life Sciences :
3001 - 3050 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
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bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... The beads were removed and the solution was further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... The DTT in the buffer for cysteine mutant of K48-diUb was removed by desalting (HiPrep 26/10 Desalting column, GE Healthcare), and the protein was reacted with the pre-mixed excess Alexa488 and Cy5 dyes ...
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bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a SuperdexTM 75 Increase 10/300 GL (GE Healthcare) size exclusion column in Tris Buffered Saline (TBS ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µL of a 10 mg/mL mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare) were added to each sample ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
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bioRxiv - Cell Biology 2020Quote: ... MFG-E8-eGFP was further purified by gel filtration on an Äkta prime system with a Superdex 200 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... 10 mM DTT and the flow-through (containing PFN1) was collected and then applied to a Superdex 75 (10/300) gel filtration column (GE Healthcare) equilibrated in 50 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were further purified by size exclusion chromatography (SEC) on a Superdex 75 Increase 10/300 GL column (GE Healthcare) for E2 cores and on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... All samples were run in a 10% polyacrylamide native gel and then imaged using a Typhoon FLA9500 gel scanner (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was then exchanged into lysis buffer supplemented with 0.15% w/v OGNG / 0.015% w/v CHS by passing over a pre-equilibrated Sephadex PD-10 desalting column (GE Life Sciences). TEV protease was added to the desalted protein at a weight ratio of 1:10 for overnight tag cleavage ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to remove remaining fluorophores and aggregates ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing plexin B2 were pooled and subjected to size exclusion chromatography with a Superdex 200 GL 10/30 column (GE Healthcare) equilibrated with Buffer C containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2020Quote: ... the expression and purification was performed as outlined by Svetlov and Artsimovitch 45 with an additional size exclusion chromatography step performed using a Superose6 10/300 GL column (GE Healthcare) pre-equilibrated with PBS Buffer at 4°C instead of an ion exchange chromatography step ...
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bioRxiv - Plant Biology 2021Quote: ... The target proteins flowed through from the column were collected and further purified by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) with lysis buffer containing 5 mM DTT (Dithiothreitol) ...
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bioRxiv - Neuroscience 2020Quote: SEC-MALS experiments of 100 μM WT and all mutants were carried out by using a Superdex 200 10/300 column (GE Healthcare) in SEC buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Biochemistry 2020Quote: ... SEC to separate oligomeric species was performed using Superdex 75 10/300GL or Superdex 200 15/150GL increase columns (GE Healthcare) in SEC buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing target protein were pooled and exchanged back to 100% buffer A using a Sephadex desalting column (HiPrep 26/10 column, GE Healthcare). TEV cleavage was performed overnight at 23°C with 1 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Immunology 2021Quote: ... The recombinant S-6P was purified by strep-tactin resin (IBA) following by size-exclusion chromatography on Superose 6 10/300 column (GE Healthcare) in 10 mM Tris pH 8.0 and 200 mM NaCl (SEC buffer) ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins (500 μl) at a concentration of 1 mg/ml were loaded on a Superdex 75 10/300 GL column (GE Healthcare) and eluted with HEPES buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Microbiology 2020Quote: Molecular mass determination was carried out using gel-filtration chromatography with a Superose 6 Increase 10/300 GL (GE Healthcare, USA) column ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
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bioRxiv - Biochemistry 2020Quote: ... The fractions corresponding to the second peak in the chromatogram (elution volume 70-75 ml) were pooled and subjected to further size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was monitored by Coomassie staining of SDS-PAGE and size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The mixed proteins were incubated on ice for 30 minutes prior to purification by size-exclusion chromatography using a Superdex 75 10/300 GL column (GE Healthcare) pre-equilibrated with a 50 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala, Sweden). All other reagents including buffers and solvents were of analytical grade.
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bioRxiv - Plant Biology 2020Quote: ... 400 μl of purified protein at 1.4 mg·ml−1 was fractionated on a Superdex 200 10/300 column equilibrated in GF buffer using an AKTA purifier (GE Healthcare) at a flow rate of 0.5 ml·min−1 ...
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bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µL of sample at 15 µM was injected from a static loop beginning at the 0 ml point of each run onto a Superdex 200 increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2020Quote: ... or Strep-Tactin XT Superflow resin (Iba) followed by a size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL -GE Healthcare) into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... IF3 was purified similarly to IF1 using a cation exchange chromatography on a HiPrep CM FF 16/10 column (28-9365-42, GE Healthcare), however using a stronger gradient in Buffer A (0.1 to 1 M NH4Cl) ...