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Citations for GE Life Sciences :
2801 - 2850 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... BSA was used for calibration and proteins were separated on a Superdex 200 Increase 10/300 analytical SEC column (GE Healthcare) with a flow rate of 0.4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... or 100 to 300 µg samples of individual proteins were injected onto a Superdex 75 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM TrisHCl ...
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bioRxiv - Biophysics 2021Quote: The HTT-HAP40 complex was further purified by size exclusion chromatography (SEC) using a Superose 6 10/300 increase column (GE Healthcare) in running buffer 25 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column into 20 mM NaPO4 150 mM NaCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: 150 μl sample was analyzed by Superose 6 Inc 10/300 column (Citiva, NY) equipped in ÄKTA purifier (GE healthcare, NY). The elution was conducted at 0.5 ml/min using the same buffer as used for sample at 4 °C
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted from Histrap HP column was further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with 10 mM maltose and further purified via gel filtration on a Superdex S75 (GE Healthcare) column.
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10−18 M).[20] EDTA was added to formulations (4 eq with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Unconjugated molecules were separated using size exclusion chromatohraphy with Superdex 200 Increase 10/300 GL column on ÄKTA fast protein liquid chromatography (FPLC, GE Healthcare). The purified nanosensors were concentrated by spin filters (MWCO = 10 kDa ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... protein-containing fractions were concentrated and further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). 7H8 was purified in SEC-150 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were concentrated and further purified by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Heparin buffer A containing 150 mM KCl ...
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bioRxiv - Biochemistry 2020Quote: ... Precipitate was removed by centrifugation at 13,000 rpm for 10 min and the soluble fraction was loaded onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare). Size exclusion chromatography was done in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... All recombinant forms of AnxA11 were subjected to size exclusion chromatography using a Superdex 75 or 200 Increase 10/300 GL column (GE Healthcare). Protein concentration was determined by absorbance at 280 nm (using molecular masses of 55513 ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Macrophages were cultured in complete medium: Dulbecco’s Modified Eagle Media (DMEM) supplemented with 10% fetal bovine serum (Hyclone, GE Healthcare, IL). Where indicated ...
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bioRxiv - Biochemistry 2020Quote: ... AcrB was then eluted with Buffer B containing 500 mM imidazole and directly injected onto a Superdex 75 10/600 GL size exclusion chromatography (SEC) column (GE Healthcare) equilibrated in buffer D (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... The protein eluent was concentrated and further purified by size-exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with W1 buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... 500 µL of each sample was individually injected into an S200 column (Superdex 200 10/300 GL, GE Healthcare Life Sciences) equilibrated in Buffer A and eluted with a flow rate of 0.5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: 100-500 µl samples (lipid-bound and lipid-free ApoE3 and ApoE380-255) were applied to an AKTA protein purification system (GE) equipped with a Superdex 200 10/300 (GE healthcare) column and a 500 µl sample loop and run at 0.5 ml/min at 5°C ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing CI2 determined from SDS-PAGE analysis were concentrated and loaded onto a superdex75 10/300 GL column (GE Healthcare) equilibrated with 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Following 3C protease treatment CDK2-cyclin E was buffer exchanged using SEC (Superdex 200 10/300) and then loaded onto a GST-trap column (5ml, GE Healthcare) and then onto an amylose resin (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: Absolute molecular weight calculations were obtained by multi angle light scattering coupled with refractive interferometric detection (Wyatt Technology Corporation) and a Superdex 75 10/300 column (GE Healthcare) at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to less than 1 mL and was injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biophysics 2021Quote: ... 500 mM imidazole) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL column, GE Healthcare). Protein concentration is determined using the protein extinction coefficient and corrected for direct absorption of the fluorophores at 280 nm ...
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bioRxiv - Biophysics 2021Quote: ... the protein was finally eluted by 230 mM imidazole and further purified through size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) with Lysis Buffer containing 0.001% LMNG ...
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bioRxiv - Plant Biology 2020Quote: ... The 200 μL of filtered solubilized fraction was used for gel filtration chromatography using the ÄKTAmicro chromatography system with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM HEPES-NaOH (pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated using an Amicon Ultra filter with 10 kDa cutoff and loaded on a HiLoad 16/600 Superdex 200 pg (GE Healthcare) size exclusion chromatography column equilibrated with SEC-buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... the clarified supernatant was purified on strep-tactin resin (IBA) followed by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 8.0 and 200 mM NaCl as the running buffer (SEC buffer) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... Sub16pro was separated from the cleaved tag by loading the protein on a Ni-NTA column and finally purified by size-exclusion chromatography over a Superdex 75 10/300 GL gel filtration column (GE Healthcare) and stored in buffer P (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Biochemistry 2021Quote: ... iMAC samples were thawed on ice and loaded into a purification column packed with 10 mL of Sephacryl S-100 (GE Healthcare) equilibrated with 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Uncleaved protein was removed by incubation with TALON Superflow resin before concentrating for loading onto a Superdex 75 Increase 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Complexes were purified by SEC on a Superdex 200 Increase GL 50/150 or a Superdex 200 Increase 10/300 GL column (GE Healthcare) and fractions containing CD4m-BG505-17b complexes were concentrated to 1.4-1.5mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Bioengineering 2021Quote: ... The biotinylation mixture was incubated at room temperature for 1 h and then loaded onto a HiPrep 26/10 desalting column (GE Healthcare) pre-equilibrated with a storage buffer (1×PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...