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Citations for GE Life Sciences :
3151 - 3200 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Proteins (40 µg) were run on 10% SDS–PAGE gel and transferred to an Amersham Hybond-ECL nitrocellulose membrane (GE healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 or 10 μg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Immunology 2022Quote: ... The eluted product from kappa affinity column was pooled and further purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... were incubated for 30 min at RT then loaded on a Superdex 200 Increase 10/300 GL column connected to an AKTA purification system (GE Healthcare). The column (bed volume 24 ml ...
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bioRxiv - Microbiology 2022Quote: ... Trimer was purified on a Superdex 200 Increase 10/300 GL column or a HiLoad 16/600 Superdex 200 PG column (GE Healthcare), whereas SApNPs were characterized on a Superose 6 10/300 GL column ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... DARPins after IMAC purification were analyzed at a concentration of 10 µM on a Superdex 75 5/150 GL column (GE Healthcare) using an Aekta Micro system (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 10 mL 1X S basal buffer for COPAS biosorter analysis and imaging on a GE InCell analyzer 2200 (GE Healthcare). See Supplementary Methods for full details.
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bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Microbiology 2023Quote: 4 mg of native protein standard was loaded to calibrate the gel filtration column (Superdex 200 increase 10/300 GL column, GE Healthcare). Following this ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham) for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... CD4-DNA conjugates were purified on a Superdex 200 Increase 10/300 GL column using an AKTA Pure FPLC (GE Healthcare). Conjugation was verified with SDS/PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2022Quote: Size-exclusion chromatography of GsSir2/Ago complexes was carried out at room temperature using Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer (20 mM Tris–HCl (pH 8.0 at 25 °C) ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions with an absorbance at 512 nm were combined and loaded as described above onto a pre-equilibrated desalting column (HiPrep 26/10 Desalting, GE Healthcare) with a desalt buffer (200 mM sodium phosphate buffer at pH 7.5) ...
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bioRxiv - Biophysics 2022Quote: ... and trimer were estimated based on a calibration curve constructed from the column manual (Superdex 75 10/300 GL, GE Healthcare). The column had been pre-equilibrated with 2 CV of running buffer (Std buffer or L-Phe buffer ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was injected into a Superdex 200 increase 10/300 GL (cytiva) column calibrated with known molecular weight standards (GE healthcare). Size exclusion chromatography was then performed at a flow rate of 0.5 ml/min in a buffer containing 20 mM HEPES pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... MBP-AIM2 labeled with peptide-Alexa 488 was purified by size exclusion chromatography (SEC) using Superdex 200 increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3C protease was removed by Ni-chelating sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The elution containing NRG1ý was concentrated with a 3K cutoff and applied on an S200 10/300 increase column (GE Healthcare). Protein content of the major peak was stored in aliquots at -80 °C for subsequent receptor purifications.
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bioRxiv - Biochemistry 2024Quote: ... The molecular weight of protein complexes was determined after size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: The quaternary structures were determined by size exclusion chromatography using a 25 mL Superdex 200 10/300 GL Increase column (GE Healthcare) for FDP_E ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... A total reaction mixture of 500 µL was used for downstream size exclusion purification utilizing a Superose 6 10/300 column (GE Healthcare) to remove excess PKA and phosphorylation reagents ...
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bioRxiv - Biochemistry 2024Quote: ... then IntC-UbcH5c-CAET-Ub was refolding and purified by size-exclusion chromatography (Superdex 75 10/300 GL column, GE Healthcare). The refolded IntC-UbcH5c-CAET-Ub-SH was mixed with IntN-RNF168 to initiate the protein trans-splicing reaction and finally obtained the RNF168-UbcH5c-CAET-Ub.
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼500 µL and loaded onto a Superose 75 10/300 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20mM HEPES,150mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... 300 mM imidazole and 0.6% C8E4 (tetraethylene glycol monooctyl ether) and concentrated before loading onto a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with buffer G containing no imidazole ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and further subjected to a size exclusion chromatography through a Superdex 200 Increase 10/300 column (GE Healthcare Life Sciences, Sweden) equipped in an AKTA FPLC system with running buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted proteins were concentrated and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2023Quote: Size-exclusion chromatography coupled with multiple angle light scattering (SEC-MALS) experiments were performed using an S75 column (Superdex 75 10/300 GL 24 ml, GE Healthcare), Agilent HPLC system (Wyatt Dawn HELIOs II ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was separated from Nb excess by size exclusion chromatography (SEC) on a Superdex-200 10/300 column (GE Healthcare). To concentrate we used protein concentrators with a cut-off of 100 kDa (Amicon ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing desired protein were pooled and concentrated using a 100 kDa centrifugal concentrator (Amicon UFC810024) and loaded on gel filtration chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) using the following buffer ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 400 mM imidazole and concentrated and applied to Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated in 0.2 M sodium bicarbonate buffer (pH 7.5) ...
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bioRxiv - Biophysics 2024Quote: ... The protein eluent was concentrated to 500 µL and subjected to size-exclusion chromatography on a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with Buffer D (25 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... TBC-DEG-αβ-tubulin assemblies were purified by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE healthcare) in binding buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The purified receptor was subsequently concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and run on size-exclusion chromatography on a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... and 40 mM DTT) after further purification by gel filtration with a Superdex 200 increase GL 10/300 (GE Healthcare, USA). Purified proteins were checked by SDS-PAGE.
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... Proteins were further purified by concentrating to ∼3mg/ml and applying to a size exclusion column (Superdex-200 increase, 10/30 GE Healthcare) equilibrated in buffer A containing 5 mM ATP ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... A total of 250 µL of each protein was injected and run on a Superdex 200 Increase (S200) 10/300 column (GE Healthcare) at a flow rate of 0.40 mL/min in SEC-MALS buffer (10 mM Tris pH 8.3 ...
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bioRxiv - Biophysics 2024Quote: ... was fed into the X-ray beam after passing through a Superdex 200 (S200) Increase 10/300 GL column (GE Healthcare) in SAXS buffer (50mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... the protein was concentrated to approximately 500 µL using centrifugal spin filters (Milipore) and injected over a Superdex 200 (S200) Increase 10/300 column (GE Healthcare) connected to an AKTA Pure FPLC (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
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bioRxiv - Microbiology 2024Quote: ... were injected first onto a His-Trap crude 1 ml column and further purified by FPLC (Äkta purifier-10, GE Healthcare) using a solution of 50 mM Na-Phosphate pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... and ClpCDWB (purified as described in Supplemental Materials and Methods) were performed on a Superose 6 10/300 Increase column (GE Healthcare) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3 and 3:1 (w/w) at a concentration of 1 mg/mL were separated on a Superdex 200 10/30 column (GE Healthcare) using a LC1100 HPLC System (Agilent Technologies ...