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Citations for GE Life Sciences :
2951 - 3000 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 5 mM EDTA were pooled and concentrated by ultrafiltration on Amicon 10 kDa cut-of membrane and loaded on HiLoad 16/60 Superdex 75 pg (#28-9893-33, GE Healthcare) equilibrated with NaAc5.5 and fractions containing the proteolytic activity on azocasein were pooled and concentrated as described above ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and further purified with size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer composed of 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... and the elution was concentrated to 1mL and loaded onto the size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with 50 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
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bioRxiv - Immunology 2021Quote: ... followed by purification to remove unbound Fab via size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare). Complexes were diluted to 30-50 µg/mL and immediately placed on 400-mesh Cu grids and stained with 2% (w/v ...
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bioRxiv - Biophysics 2020Quote: Size-Exclusion Chromatography-Multi Angle Light Scattering (SEC-MALS) experiment was performed at 25°C using a Superdex 200 10/300 GL column (GE HealthCare) connected to a miniDAWN-TREOS light scattering detector and an Optilab T-rEX differential refractive index detector (Wyatt Technology ...
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bioRxiv - Microbiology 2020Quote: ... concentrated using a Vivaspin 20 centrifugal concentrator (10-kDa molecular weight cutoff) and applied to a HiLoad 16/600 Superdex 75 pg gel filtration column (GE Healthcare). Pure protein samples were dialyzed against 10 mM TrisHCl pH 7.0 and concentrated as described above ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Neuroscience 2021Quote: ... to a final volume of 300 μl and incubated with 10 μl of medium slurry of streptavidin-coated magnetic beads (GE Healthcare) for 2 hours at room temperature under continuous rotation ...
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 5-fold with buffer A (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A supplemented with 100 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 3-fold with dilution buffer (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare). Entry routes were determined by pre-treating monolayers of VeroE6 or VeroE6-TMPRSS2 cells with a concentration range of camostat mesylate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 mm; GE Healthcare). All pseudovirus experiments were quantified using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were further concentrated and purified by size-exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare), pre-equilibrated with SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution fractions were loaded onto a SuperoseTM6 10/300 GL column previously equilibrated with buffer L using an ÄKTA Pure system (GE Healthcare). The purified His-SctV9-PR Peptidisc complex was subsequently analyzed by negative-stain electron microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing mainly His-SctV-C9 were further purified on a Superdex200 10/300 GL column previously equilibrated with buffer M using an ÄKTA Pure system (GE Healthcare). His-SctV-C9 complexes were subsequently analyzed by cryo-EM.
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bioRxiv - Biochemistry 2021Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD proteins were further purified with size-exclusion chromatography using a Superdex 200 Increasse 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The AfAgo+MZ-1289 was concentrated to 175 μM and loaded on the column Superdex 200 Increase 10/300 (GE Healthcare) equilibrated with the sample buffer ...
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bioRxiv - Biophysics 2021Quote: ... The elution containing NRG1β was concentrated with a 3K cutoff and applied on an S200 10/300 increase column (GE Healthcare). Protein from the major peak was stored in aliquots at −80 °C for subsequent receptor purifications ...
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bioRxiv - Biophysics 2021Quote: ... The mix was allowed to incubate for 1 hour over ice and loaded to a Superdex 200 Increase 10/300 GL size exclusion column (GE healthcare) pre-equilibrated in buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The his-tagged protein was eluted 4 CV of elution buffer (lysis buffer supplemented with 250 mM imidazole) and further purified by size exclusion chromatography in a Superdex 200 10/30 (GE Healthcare) equilibrated with 20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... concentrated using a 30 kDa molecular weight cut-off (MWCO) Ultra Centrifugal tube (Amicon) and loaded to a Superose 6-Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed and His-ALOD4 and His-OlyA were isolated by nickel purification followed by size exclusion chromatography (HisTrap-HP Ni column, Tricorn 10/300 Superdex 200 gel filtration column; FPLC AKTA, GE Healthcare). Protein-rich fractions were pooled and concentration was measured using a NanoDrop instrument.
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was also evaluated via size exclusion chromatography by loading 400 µl of the Hitrap SP HP purified fraction onto Superdex 200 increase 10/300 GL column (GE Healthcare), and a monodisperse peak was obtained at 11.8 ml elution fraction.
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bioRxiv - Biochemistry 2022Quote: ... Anion exchange chromatography was performed on an ÄKTA Pure system equipped with a 10/100 Tricorn column (Cytiva) packed with Source 15Q ion exchange resin (Amersham Biosciences, GE Healthcare) equilibrated in AEX buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... 3C protease was removed by Ni-chelated sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... 50-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 75 10/300 GL increase size-exclusion chromatography column (GE Healthcare) in 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorophores and other small molecules were removed from the proteins by passing the reaction mixture through a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with the Protein Buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the TNC buffer in which proteins were stored was exchanged using Sephadex G-25M PD-10 desalting gravity columns (GE Healthcare) into 50 mM N-Cyclohexyl-2-aminoethanesulfonic acid (CHES ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2019Quote: ... aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare). aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA ...
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bioRxiv - Biophysics 2019Quote: ... Before experiments 1 mL of aSyn was further purified using a Superdex 75 pg 10/300 GL size exclusion chromatography (SEC) column (GE Healthcare) to obtain monomeric protein ...
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bioRxiv - Cell Biology 2019Quote: ... PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM PIPES pH 6.8 ...
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bioRxiv - Biochemistry 2019Quote: ... the peak size-exclusion chromatography F86E RAD51 elution fraction was serially diluted (5, 1:1 serial dilutions) and loaded onto a Superdex200 10/300 GL size exclusion column (GE Healthcare) equilibrated with MALS Buffer (150 mM KCl ...
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bioRxiv - Biochemistry 2019Quote: ... ~300 μL of 5 μM complex were mixed at a 1:1 molar ratio and incubated for 30 minutes at 4° before injection onto a Superdex200 10/300 GL analytical scale column (GE Healthcare). For CRY:PER2 CBD complexes used for BLI or CRY1:PER2 CBD:CLOCK:BMAL1 bHLH-PAS:Ebox DNA for cryo-EM ...
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bioRxiv - Molecular Biology 2019Quote: ... A 10 μl aliquot of each reaction mixture was loaded on polyethyleneimine (PEI) cellulose thin-layer chromatography (TLC) plates (GE Healthcare) and separated by chromatography in 1.5 M potassium phosphate at pH 3.4 ...
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bioRxiv - Plant Biology 2019Quote: ... and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns, GE Healthcare).