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Citations for GE Life Sciences :
2701 - 2750 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein base buffer without imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... The concentrated flowthrough was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL Increase (GE Healthcare) column using 20 mM tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Mixtures were incubated at room temperature for 20 minutes and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with assembly buffer ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Biophysics 2020Quote: The oligomerization states of A3H Hap I mutants were determined by loading 120 to 300 μg of the purified enzymes onto a Superdex 200 10/300 Increase (GE Healthcare). The running buffer contained 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: Full-length PbCSP was co-complexed with a molar excess of 3D11 Fab and loaded on a Superose 6 Increase 10/300 GL (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Microbiology 2020Quote: ... the released proteins were further purified on a Superdex 200 (for nsp12) or a Superdex 75 (for nsp7 and nsp8) Increase 10/300 GL column (GE Healthcare) using 25 mM HEPES–NaOH ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated by ultrafiltration (Amicon, 10 kDa cutoff,) before further purifying on a Superdex S75 16/60 gel filtration column (GE Healthcare) in 25 mM NaH2PO4/Na2HPO4 pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to nitrocellulose (GE Healthcare. For preparation of cell wall extracts for mass spec ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration chromatography was performed using an AKTA FPLC System with a Superose 6 Increase 10/300 GL column (GE Healthcare) in 50 mM NaPO4 pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 10 mM maltose solution in the lysis buffer and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) before being subjected to additional purification by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... experiments coupled to multi-angle laser light scattering (MALS) and refractomestry (RI) were performed on a Superdex S200 10/300 GL increase (GE Healthcare) for NyxA and a Superdex S75 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... or Protein A resin (Pierce) before being subjected to additional purification by size-exclusion chromatography using either a Superose 6 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated TANV16L was subjected to size-exclusion chromatography using a Superdex S200 increase 10/300 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... heated to 95°C for 5min and separated on a 10% SDS-PAGE gel before transferring onto a nitrocellulose membrane (Amersham Hybond ECL, GE Healthcare) using BioRad transfer apparatus ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2019Quote: We first performed buffer exchange of the purified BamAB-RcsF complex using a PD-10 desalting column (GE Healthcare Life Sciences). The complex was eluted with 20 mM HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 nvRNAP core sample was polished and buffer-exchanged using size exclusion chromatography on a Superdex 200 10/300 (GE Healthcare) column equilibrated with buffer D containing 100 mM NaCl ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Molecular Biology 2022Quote: Hela and Hela-RNAseH1 cells downregulated for the indicated genes were grown in the presence of 10 μM bromodeoxyuridine (BrdU, GE Healthcare) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Plasma was separated by centrifugation at 1100 rpm for 10 minutes and PBMC was isolated from the buffy coat by Ficoll-Paque PLUS (GE Healthcare) and cryopreserved in fetal calf serum + 10% DMSO.
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bioRxiv - Biophysics 2022Quote: ... The protein of interest was separated by affinity chromatography using resin attached with Ni-NTA and being subjected to additional purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 and 200 mM NaCl.
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 and 2E10.E9 Fab-PvCSPvk247 complexes were purified via Superose 6 Increase 10/300 GL chromatography (GE Healthcare) and concentrated to 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: Full-length recombinant PvCSPvk210 or PvCSPvk247 was complexed with a molar excess of 2F2 or 2E10.E9 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 or 2E10.E9 Fab-PvCSPvk247 complexes were purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) and diluted to 50 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluate was concentrated to below 500 μL and subjected to size-exclusion chromatography on a Superdex 200 10/300 Increase Analytical (S200a) column (GE Healthcare) equilibrated with 10 mM HEPES (pH 7.7) ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... HspA1A-containing fractions were once again combined and loaded onto a Superdex™ 200 Increase 10/300 GL (GE Life Sciences) size exclusion column ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was passed through a HiLoad 10/300 Superdex 75 GL size exclusion column using an ÄKTA pure purification system (GE Healthcare). Peak fractions were pooled and concentrated using Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples of TRIM21 CC 235 (100uL) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min in 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times with TBS-T for 10 min each before incubation with Amersham ECL detection reagent (GE Healthcare) and chemiluminescent detection using the Li-COR Odyssey FC imaging system (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing EftMHM4 or EftMPAO1 were pooled and the protein further purified by gel filtration chromatography on a Superdex200 10/300 column (GE Healthcare) equilibrated with gel filtration buffer containing 50 mM Tris pH 7.5 ...