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Citations for GE Life Sciences :
2851 - 2900 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... the eluants were further applied to size exclusion chromatography with the Superdex 75 or Superdex 200 column with ÄKTA Purifier 10 FPLC system (GE Healthcare). The E ...
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bioRxiv - Plant Biology 2020Quote: ... PAIPP2-3FLAG and CPL2-4MYC with IP buffer and then loaded on to a Superdex 200 10/300GL column (GE Healthcare). The eluted fractions were collected in 96-well plates and the target proteins were detected by standard western blotting.
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bioRxiv - Microbiology 2020Quote: ... Biotech AB/Sweden) prior to protein separation on a S200 Superdex 10/300GL column on an Äkta Purifier (both GE Healthcare). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:10,000 in TBS-T with 3% BSA and finally washed 3 x in TBS-T for 10 min before adding ECL substrate for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... Cleaved proteins were subsequently purified from the protease and affinity tag by a second Ni2+-affinity column or by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare). Proteins were concentrated to 3–10 mg/ml and either used immediately or aliquoted and quickly frozen at –80°C ...
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bioRxiv - Microbiology 2020Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) in a 25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Plant Biology 2021Quote: ... using an AKTA Pure 25 system equipped with HisTrap HP 1 mL column and submitted to gel filtration using a Superdex 200 increase 10/300 GL column (GE healthcare). Purified recombinant proteins were conserved at −80°C in 1x PBS solution containing 10% glycerol.
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bioRxiv - Microbiology 2020Quote: ... The bacteria lysate containing GST-Avr1d was incubated with 10 μl glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare) for 3 hours and purified by washing with 1×PBS for 5 times ...
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bioRxiv - Microbiology 2020Quote: ... The methylated VSG13 N-terminal domain was purified by gel filtration on a Superdex 200 Increase 10/300 GL (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Microbiology 2021Quote: PdeL samples (100 µl) varying from 11 to 371 µM were loaded onto a Superdex 200 (10/300) column (GE Healthcare) at constant flow (0.5 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... the eluted proteins were concentrated to ∼200 µL and purified by size-exclusion chromatography on a Superose 6 10/300 GL column (GE Healthcare) in 10 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The chymotrypsin-treated protein was further purified by size exclusion chromatography using a Superdex 200 HR 10/30 column with an AKTA FPLC system (GE Healthcare) in buffer C (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... were used depending on the enzyme availability) and were further purified by gel-filtration chromatography on Superdex 200 10/300 column (GE Healthcare) in Buffer A (20 mM HEPES-KOH pH7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 66 kDa) were used to generate a molecular weight standard plot using a Superdex 200 10/300 GL column (GE Healthcare). A 3.2 mg/ml sample of His-PodJ(1-635 ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The sample (100 µL of 1 mg/mL UL21-H6) was injected onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in 20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... HSF2BP and ARM proteins were loaded in the presence and absence of the BRCA2 fragment F15X on a Superdex 200 10/300 GL (GE Healthcare) using a HPLC Shimadzu system coupled to MALS/QELS/UV/RI (Wyatt Technology) ...
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bioRxiv - Molecular Biology 2020Quote: ... divided into aliquots of 10 mL and each aliquot was added on top of a 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of each reaction mixture was loaded on polyethyleneimine (PEI) cellulose thin-layer chromatography (TLC) plates (purchased from GE Healthcare) and separated by chromatography in 1.5M potassium phosphate at pH 3.4 ...
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bioRxiv - Microbiology 2020Quote: ... Purified protein obtained using glutathione affinity chromatography was then subjected to size-exclusion chromatography using a Superdex S75 10/300 or 16/600 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and 10 ug were separated on an 0.8% agarose gel and transferred to a Nylon membrane (GE Healthcare Limited, Buckinghamshire, England) as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... Analytical size exclusion chromatography using a Superdex 200 Increase 10/300 column confirmed the absence of aggregates from the protein preparation (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... and fractionated by linear gradient with end buffer (10 mM Tris-HCl, 1 M NaCl) using an AKTAprime plus (GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Sample volumes of 100 μl of APT1 WT and ATP2 WT at 40 μM were injected into a Superdex 75 10/300 GL column (GE Healthcare) previously equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer C with 0.005% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... concentrated to < 1 mL by centrifugal ultrafiltration (10,000 or 5,000 MWCO ultrafilter) and loaded onto a Superdex 75 Increase (10/300 GL) column (GE Healthcare Life Sciences) equilibrated in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluates were subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... A2AAR reconstituted into nanodiscs was subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: Size-exclusion chromatography was carried out using a Superdex 75 (HR 10/30) coupled to an Äkta purifier system (GE Healthcare), equilibrated with a 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were concentrated using a 50-kDa MWCO concentrator (Amicon) and further purified Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... but no 3C protease cleavage was performed and the protein was further purified on a Superdex 200 Increase 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... The eluted mutant was then applied to a Superdex 200 10/300 GL size-exclusion chromatography column (Cytiva, Tokyo, Japan (GE Healthcare)) to isolate the monodisperse fraction of the AaRseP mutant ...
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bioRxiv - Biophysics 2021Quote: ... 100-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 200 or 75 10/300 GL Increase size-exclusion chromatography column (GE Healthcare) in 20 mM Tris HCl ...
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bioRxiv - Biophysics 2020Quote: ... Plexins and GTPases were further purified by either size exclusion chromatography or using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biophysics 2021Quote: ... Insoluble material was removed by ultracentrifugation at 150,000 x g for 20 min before purification by SEC on a Superpose 6 increase 10/300 GL column (GE Healthcare) equilibrated with buffer (25 mM HEPES ...