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Citations for GE Life Sciences :
2751 - 2800 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... were analyzed in aliquots of 50 μl each at a sample concentration of 2 mg/ml (in 20 mM Hepes pH 7.5, 150 mM NaCl) on a Superdex 200 10/300 increase column (GE Healthcare, Germany) at a column temperature of 35 °C and a flow rate of 0.7 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... no spectral changes were observed for WT protein anymore and the sample was applied to a size exclusion column (HiLoad 10/300 Superdex 75 pg column, GE Healthcare) and the dimeric fraction of about 50% of the total protein was pooled ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel was processed for Southern blotting: DNA was transferred on nylon membrane by passive transfer in 10×SSC buffer (Amersham Hybond XL, GE Healthcare). Membranes were UV-cross-linked and hybridized overnight with 32P-labeled single-stranded DNA probes at 42 °C ...
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Plant Biology 2019Quote: ... Purification tags were removed by incubation with 3C protease (10 μg/mg fusion protein) followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/60 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was loaded at concentrations ranging from 2 to 16 mg/ml on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated with a Vivaspin 100-kDa MWC0 concentrator and the monomeric FPN fraction was collected after size-exclusion chromatography (SEC) over a Superdex S200 Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.50 ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged for 10 min at 5000 g and the lysate was passed through a Source-15Q anion exchange 55 ml column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... All affinity-purified proteins were size-exclusion-purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare, catalog 28990944) in HEPES buffered saline (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2019Quote: ... The labeling reaction was quenched after 1 hr with fresh DTT at 100 mM final concentration and loaded onto a HiPrep 26/10 desalting column (GE Healthcare). The final labeled proteins were concentrated to at least 80 µM and flash-frozen in Protein Buffer (50 mM Tris-HCl [pH 7.2] ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein was further purified using size-exclusion chromatography with a Superdex 75 10/300 GL column (GE Life Sciences) equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were further purified by size-exclusion chromatography (on either a Superdex 200 increase 10/300 GL or a HiLoad 16/600 Superdex 200pg column, both GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The HiTrap ButylFF column of 1 mL and the gel filtration column Superdex 200 10/300 GL were from GE Healthcare. MacroPrep HighS resin was from Biorad ...
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... His6-MBP-TEV-NRBF2MIT was concentrated with a 10 kDa MWCO Amicon concentrator and injected over a Superdex-200 column (GE Healthcare) equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... CDK1/CycA was concentrated and the holocomplex separated from free CDK1 by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare). Peak fractions were analyzed by SDS-PAGE analysis and fractions where CDK1 and CycA co-eluted were pooled ...
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bioRxiv - Microbiology 2019Quote: ... The holo-UbiV or holo-UbiU/V complexes were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer C or A ...
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bioRxiv - Biochemistry 2019Quote: ... The pooled protein was further purified by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare Life Sciences). MPEG-1 fractions were monitored using SDS-PAGE ...
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bioRxiv - Biophysics 2019Quote: ... the flow through from this step was concentrated using a centrifugal concentrator and applied to a Superdex200 10/300 GL (GE Healthcare) size exclusion column equilibrated with a “Standard” buffer of 20 mM Tris-HCl ...
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bioRxiv - Immunology 2019Quote: ... the cell suspension diluted in 1 mL of complete L15 medium was carefully layered on top of 5 mL of Percoll gradient (L15/10 mM HEPES/20% Percoll (GE Healthcare) and centrifuged at 400 g for 9 min ...
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... USA) and an Optilab T-rEX refractometer (Wyatt Technology) in-line with a Superdex 75 10/300 GL (GE Life Sciences) size exclusion chromatography analytical column ...
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bioRxiv - Biophysics 2019Quote: ... The dissolved peptide was then fractionated on a Superdex-75 HR 10/30 size exclusion chromatography (SEC) column (GE Life Sciences) on a BIORAD FPLC system that was pre-equilibrated with 20mM Tris at pH 8.00 ...
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bioRxiv - Biochemistry 2019Quote: ... and cleaved protein was concentrated and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... Fractions passed through the HisTrap HP column were collected for further purification with the Superdex 200 Increase 10/300 GL column (GE Healthcare).
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The reaction mixture was then used for purification through size-exclusion chromatography with Superdex 200 Increase 10/300 GL column (GE Healthcare) in buffer A to remove extra σ28 protein and nucleic acids.
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bioRxiv - Molecular Biology 2019Quote: ... from mouse liver was separated by 10% denaturing polyacrylamide gel electrophoresis (PAGE) and blotted onto a nylon membrane (Amersham Hybond N+; GE Healthcare) using a Transblot Turbo apparatus (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with a 50 kDa MWCO concentrator and subjected to a second SEC on a Superose 6 (10/300 GL, GE Healthcare) with a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the V2R was purified by size exclusion chromatography (SEC) using a Superdex 200 (10/300 column) connected to an AKTA purifier system (GE Healthcare). Fractions corresponding to the pure monomeric receptor were pooled (~ 2 ml ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was concentrated in a 100 kDa cut-off Vivaspin® 2 Centrifugal Concentrator to a final volume of less than 500 μl before injected onto a Superdex 200 10/300 Increase GL column (GE Healthcare), which was equilibrated with GF buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... with a molecular weight cut-off (MWCO) of 30 kDa and polished by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: Multi-angle light scattering coupled with size exclusion chromatography (SEC-MALS) was done using a Superdex200 Increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml min−1 on an HPLC system composed of PU-2080 pumps ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were resolved by SDS-PAGE using a 10 % acrylamide gel and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Amersham Hybond, GE Healthcare) via the PerfectBlue™ tank electro blotter (Peqlab ...
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bioRxiv - Biophysics 2021Quote: ... The H2A.Z(K120C)-H2B made by combining cell pellets was also purified further by size exclusion chromatography on an S75 column (Superdex 75 Increase 10/300 GL, GE Healthcare) before storage.
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant obtained at this step was filtered through 0.45 μm filters and injected into a sample loop connected to HiPrep Q FF 16/10 (GE healthcare, 28936543). The supernatant was injected at 2 mL/min and eluted using buffer B (20mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... centrifuged at 10000g for 5min to remove precipitation and the soluble fraction was injected on a Superdex 75 10/300 (GE Healthcare). To refold EH domains ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were further purified by SEC on a Superdex 200 Increase 10/300 GL column or HiLoad Superdex 200 16/600 column (GE Healthcare). GPΔmuc-presenting NPs were produced in ExpiCHO cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...