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Citations for GE Life Sciences :
3051 - 3100 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Pathology 2019Quote: ... proteins were separated based on their isoelectric point on 18-cm immobilized pH gradient (IPG) gel strips of pH 3-10 (GE Healthcare). Subsequent to overnight rehydration ...
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bioRxiv - Genetics 2019Quote: ... The purified DNA samples were subjected to 10% denaturing polyacrylamide gel electrophoresis and were detected using the Typhoon FLA9500 (GE Healthcare) and analyzed with the ImageQuant TL software (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Protein samples (each protein at a concentration of 40 μM) were loaded to a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with assay buffer (same with ITC).
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bioRxiv - Biochemistry 2019Quote: H2Bub-containing octamers were assembled using the salt dialysis method (Dyer et al., 2004) and purified over a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and allowed to mix at room temperature for 30 minutes before injection on to a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The product was purified from the cell culture medium by affinity chromatography using a Protein A affinity column and analyzed by SDS-PAGE, Size Exclusion Chromatography (Superdex200 10/300GL, Healthcare) and Surface Plasmon Resonance analysis (Biacore S200, GE Healthcare) on an EDB antigen-coated sensor chip (CM5 ...
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bioRxiv - Biophysics 2019Quote: ... histone protein octamer was reconstituted from denatured purified histones via refolding in high salt buffer and purified on a Superdex 200 increase 10/300 GL size exclusion column (GE Healthcare). DNA containing the Widom 601 positioning sequence with 60bp of flanking DNA was made by large scale PCR with Taq DNA polymerase and purified by native PAGE as described previously (Zhou and Narlikar ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was concentrated to 500 µl and subjected to size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare) in gel filtration buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Micrographs in time frames of 10 min were acquired during 24 h in a Deltavision RT imaging system (Olympus IX70/71, Applied Precision) equipped with a Plan apochromatic 20X/1.42 N.A ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Biochemistry 2020Quote: P3 membranes were solubilized as described in the previous section and 200 μL of the detergent-solubilized supernatant was injected in a Superose 6 10/300 GL gel-filtration column (GE healthcare) equilibrated with 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved eluate was concentrated using a centrifugal filter (50 kDa cut-off, Amicon) and then loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare). The peak fraction containing recombinant SKI complex was collected ...
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bioRxiv - Biochemistry 2020Quote: ... containing 0.05 M NaCl to a final concentration of 1 mg/ml was applied to a Superdex 10/300 column (GE Healthcare) run at a flow rate of 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions of protein were then concentrated and loaded onto a 10/300 Superdex200 Increase gel filtration column (GE Life Sciences) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was concentrated and purified by size exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare) on ÄKTA™ Pure ...
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bioRxiv - Biochemistry 2021Quote: MALS was measured using a DAWN HELEOS-II system (Wyatt Technology Corporation) downstream of a GE liquid chromatography system connected to a Superdex 200 10/300 GL (GE Healthcare) gel filtration column ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... fused to lysozyme and BRD4-ET either alone or together was evaluated by injecting each purified protein onto a Superdex 75 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with 20 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... eluted fraction were set aside after the first step of Ni affinity chromatography and directly loaded on a Superdex 200 Increase (10/300 GL) column (GE Healthcare) and eluted in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fraction was concentrated and further purified by size exclusion chromatography using an S200 increase (10/300 GL) column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... The target proteins flowed through from the column were collected and further purified by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) with lysis buffer containing 5 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted supercomplex solution was concentrated as described above and further purified by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare), preequilibrated with buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Oligomerization of delVirB4_CTn4 and delVirD4_CTn4 proteins was studied by gel chromatography on Superdex200 10/30 GL column connected to ÄKTA Explorer liquid chromatography system (GE Healthcare). The proteins ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Biophysics 2021Quote: ... Purified TolC and colicin E1-T or -TR were mixed at a 1:2 molar ratio to ensure an excess of colicin to saturate TolC binding sites and incubated at room temperature for 1 hour before loading onto a Superdex 200 Increase 10/300 GL column (GE Healthcare). The protein was eluted with 1.5 column volumes into 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The LFT-μOR-Gi1-scFv16 complex was further purified by size exclusion chromatography on a Superdex 200 10/300 GL column (GE healthcare) in 20 mM HEPES 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... purified fluorescent ULK1 complex (FIP200-MBP/EGFP-ATG13/EGFP-ATG101/ULK1) was mixed with purified GST-NDP52 or GST-4xUb and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences) coupled to an Äkta purifier system (GE Healthcare). To perform the subsequent experiments ...
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bioRxiv - Biophysics 2021Quote: ... Fractions showing the corresponding encapsulin band on SDS-PAGE were pooled and subjected to further purification via anion-exchange chromatography using a HiPrep Q 16/10 column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Nap1 containing fractions were concentrated to 2 mg/mL and loaded on a Superdex 200 10/300 GL gel-filtration column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing purified FliF RBM2 (FliF124-229) were further purified by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare) in a buffer containing 50 mM Hepes pH 9.0 ...
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bioRxiv - Microbiology 2021Quote: ... the released proteins were further purified on a Superdex 200 (for nsp12) or a Superdex 75 (for nsp7, nsp8 and NiRAN) Increase 10/300 GL column (GE Healthcare) using 25mM HEPES–NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... size exclusion chromatography (SEC) coupled to MALS analysis was performed on MiniDAWN TREOS II coupled to S200 10/300 increase column (GE Healthcare) mounted on FPLC system (AKTA purifier) ...
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bioRxiv - Biophysics 2021Quote: ... using a Shimadzu HPLC LC-20AD/RF-20A (488 nmex, 512 nmem) instrument and Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... These solutions were prepared either by dialysis against the buffer at 4°C or by passage through PD-10 desalting columns (GE Healthcare). Protein concentrations were measured spectrophotometrically using known values for the extinction coefficient ...