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Citations for GE Life Sciences :
3101 - 3150 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare). Immunoprecipitation was performed overnight by incubation with anti-V5 antibody ...
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bioRxiv - Biophysics 2020Quote: Proteins were further purified by size-exclusion chromatography (Superdex 200 HiLoad 26/60 or Superdex S200 Increase 10/300 GL; GE Healthcare) on an AKTA pure fast protein liquid chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Fab and scFv constructs were eluted from affinity purification and then purified further via size exclusion chromatography using the Superdex 200 Increase 10/300 GL column (GE HealthCare) and 1X PBS ...
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bioRxiv - Microbiology 2020Quote: The Vag8:C1-INH complex was prepared in vitro by incubating C1-INH in ∼1.5 molar excess with Vag8 at room temperature for 10 min followed by purification using size exclusion chromatography on a S200pg 16/600 column (GE Healthcare). The eluted fractions were analysed by SDS-PAGE followed by ultrafiltration to concentrate the protein complex.
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bioRxiv - Microbiology 2020Quote: ... Germany) and differentiated into macrophages by culture for 7 days in RPMI1640 medium supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Properly folded trimers were eluted with 3M MgCl2 and immediately dialyzed into storage buffer before being subjected to multiple size exclusion chromatography runs with a Superdex200 16/60 column followed by a Superose6 10/300 column (GE Healthcare). Peak fractions verified to be BG505 SOSIP.664 trimers were stored as individual fractions at 4°C in storage buffer.
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bioRxiv - Microbiology 2020Quote: ... fluorescently labeled R18-HRSV or culture supernatants of HEp-2 were separated from excess R18 by a separation column (PD-10 Desalting Columns GE Healthcare). HRSV-R18 or culture supernatants of HEp-2 were incubated in suspension with A3.01 and HEp-2 cells at 4°C for 1 h to allow virus attachment ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a Superdex 75 Increase 10/300 GL (GE Healthcare) size exclusion column in TBS (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The glutathione Sepharose column eluate was then concentrated to 500 μL and passed over an S75 10/300 size exclusion column (GE healthcare) with 20 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare). Protein standards from Bio-Rad were used to interprete elution profiles ...
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bioRxiv - Immunology 2020Quote: ... The sample was concentrated using Vivaspin-20 10 kDa and further purified via SEC using ÄKTA Pure 25 (GE Life Sciences) equipped with Superdex 75pg 16-600 column (GE Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... concentrated and dialyzed into 1x PBS buffer before being further purified by size exclusion chromatography (SEC) using Superdex 200 10/300 GL column (GE healthcare). Identified SEC eluate fractions were pooled and concentrated to 1mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-eluted motor domains (wild-type + jordan) were bound to a strong anion exchanger (5/50 MonoQ GL; Cytiva) using a Purifier 10 chromatography system (GE Healthcare). The column was washed with MOPS (10) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared through a Millipore 0.22 micron Durapore centrifugal filter and applied to a Superdex-200™ (10/300 GL) column (GE Healthcare) which was pre-equilibrated and then run in gel filtration buffer (TBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 kDa molecular weight cutoff) and purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), which was pre-equilibrated with buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was concentrated with a 100-kDa cut-off centricon (Milipore) and further purified by Superose-6 increase 10/300 column (GE Healthcare) using a buffer containing 50 mM Imidazole pH 6.0 ...
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bioRxiv - Microbiology 2020Quote: ... Fractions corresponding to assembled nanoparticles (elution between 8.5-10.5 mL with a peak at 9 mL) were pooled and concentrated at 350 × g by spin filtration with Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare). 16055 SOSIP-I53-50NPs were then buffer exchanged into PBS supplemented with 250 mM sucrose using a Slide-A-Lyzer MINI dialysis device (20 kDa molecular weight cutoff ...
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bioRxiv - Microbiology 2022Quote: ... Eluted mAb92 was further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were concentrated to 500 µl by Amicon Ultra 15 concentrators with 30 kDa cutoff and applied to a Superdex 200 10/300 Increase gel filtration column (GE Healthcare). Individual fractions containing the NTDyn and NDD were pooled and concentrated at 10 mg/ml.
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bioRxiv - Molecular Biology 2022Quote: ... SEC-MALS of GsSir2/Ago and CcSir2/Ago complexes was performed at room temperature using Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer (20 mM Tris–HCl (pH 8.0 at 25 °C) ...
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bioRxiv - Biochemistry 2022Quote: ... in 1 ml fractions and protein containing fractions were applied to SEC without further concentration using a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which was equilibrated in SEC buffer.
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 9,000 g for 10 min and supernatant was collected and mixed gently with 200 µl of Ni Sepharose High Performance (GE Healthcare) and incubated for 1 h in same condition as above ...
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bioRxiv - Biochemistry 2022Quote: ... HIV-1 IN was further fractionated by size exclusion chromatography (SEC) with a Superdex 200 Increase (10/300) column (GE Healthcare) and stored at −80°C (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... with a molecular weight cut-off (MWCO) of 30 kDa and polished by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Bioengineering 2022Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10-18 M).20,21 EDTA was added to formulations (4 molar equivalents with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Biophysics 2022Quote: ... This was followed by size exclusion chromatography (SEC) using 24 ml Superdex 200 Increase 10/300 GL column on an AKTA-FPLC system (GE Healthcare). Analytical gel filtration was performed in 20 mM Tris pH 7.5 and 150 mM NaCl at the flow rate of 0.5 ml/min.
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bioRxiv - Biophysics 2022Quote: ... All the data were collected in SEC-SAXS mode with an in-line Superdex 200 Increase 10/300 GL column (GE Healthcare). Both proteins were concentrated to 8 mg/mL and centrifuged at 20,000 x g immediately before injecting the protein onto the column ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biophysics 2022Quote: ... 32 and purified according to an established protocol 32 with the exception that the Mono Q® ion-exchange purification step was exchanged with a size exclusion chromatography step using a Superose6 10/300 GL column (GE Healthcare) pre-equilibrated with PBS buffer 33 supplemented with 2 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were concentrated and further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare) pre-equilibrated with 100 mM HEPES (pH 7.0) ...
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bioRxiv - Bioengineering 2022Quote: ... all proteins were then further purified by size exclusion chromatography using a Superdex 75 10/300 GL increase column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: SEC experiments were performed on 100 μL injections of 70 μM ScMlp11-325 with a Superdex 200 (10/300) GL column (GE Healthcare) at 0.5 mL min−1 at 25°C in 500 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Activated C1s was then separated from C1r using an analytical size exclusion chromatography column Superdex 200 Increase 10/300 GL (GE Healthcare) in HBS ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were then exchanged into native binding buffer using a HiPrep Desalting 26/10 column on an ÄKTA pure FPLC (GE Healthcare). N-terminal tags were removed from Elp proteins using His-tagged tobacco etch virus (His-TEV ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Biochemistry 2022Quote: ... Removal of free dyes outside liposomes and exchange of outside buffer was achieved by passing samples through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... All images were recorded at a frame rate of 50-80 frames per second were stored for postprocessing analysis with QLAB 10 CMQ (Philips) advanced quantification software (EchoPAQ, 110.1.2 GE Healthcare, Horten, Norway)
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bioRxiv - Plant Biology 2022Quote: The purified protein from untagged OscobB was further analysed by a size exclusion chromatography (SEC) passing 0.5mg protein through the SUPERDEX 200 (10/300 GL) column (GE Healthcare, USA) attached to AKTA pure 25 at the rate of 1ml/min ...
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bioRxiv - Microbiology 2022Quote: ... Eluates were concentrated by centrifugal filtration and proteins were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with running buffer PBS ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were harvested and filtered for further purification by Histrap HP (GE) and Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...