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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared by centrifugation and incubated at 4°C for 1.5–2 h with anti-FLAG M2 affinity gel (Sigma). After extensive washing with native IP buffer ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The stained tissue sections were scanned using an Olympus VS-120 slide scanner and imaged using a Hamamatsu ORCA-R2 C10600 digital camera for all bright-field and fluorescent images ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Neuroscience 2020Quote: (T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3’5-dionato-kO3’kO5] difluoroboron (CRANAD-2) [26] was purchased from Sigma-Aldrich AG ...
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bioRxiv - Microbiology 2021Quote: ... and the reaction was started with 100 μl of 4 mg/ml 2-nitrophenyl β-galactopyranoside (ONPG, Sigma-Aldritch) in Z buffer and stopped with 250 μl of 1M Na2CO3 ...
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bioRxiv - Neuroscience 2020Quote: ... 2-4 day old adult males raised in normal cornmeal food were transferred to food containing RU486 (mifepristone, Sigma) in 80% ethanol to a final concentration 200 μg/ml or with the same amount of ethanol (vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... on ice and in a cold room at 4°C for 2 hours then warmed up to 37°C in complete medium with 10μM Nocodazole (M1404, Sigma) and incubated until fixation ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: Mice aged 6 months or naked mole-rats aged 2-4 years were given intraperitoneal (i.p.) injections with 150mg/kg 5-Fluorouracil (5-FU; Sigma) from a 50mg/ml stock in DMSO diluted with sterile 0.9% sodium chloride solution (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 xg at 4°C for 2 min and Pah1-FLAG was immunoprecipitated with anti-FLAG agarose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... The fixed animals were then washed twice with PBSTx1 and stained with 1μg/mL DAPI (4’,6-diamidino-2-phenylindole)(Sigma) solution for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times and stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with 4’,6-amidino-2-phenylindole (DAPI) and mounted with polyvinyl alcohol with DABCO (PVA-DABCO; Sigma). A list of antibodies and concentrations used for IF can be found in Supplementary Table 4 ...
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bioRxiv - Neuroscience 2021Quote: Rat and mouse primary cortical neurons and BE(2)-M17 cells were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS pH 7.4 for 20 minutes at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were washed with TBST and incubated with 300 nM 4‘,6-diamidino-2-fenilindol (DAPI, D9542, Sigma-Aldrich) for 1 minute ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured for 72 h and then cell death was measured using the tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-terazolium-5-carboxanilide (XTT) assay (Sigma) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... and non-selective P2-purinoreceptor antagonist pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid (PPADs) ([25mg/kg] in PBS; Sigma).27,44–46 Following recording of a pre-inhibitor response ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed three time in PBS and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Coverslips were mounted onto glass slides in Mowiol-DABCO ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were prepared in PBS and subsequently fixed for 4 h at RT in fixative (2% glutaraldehyde (Sigma, Germany)/ 4% paraformaldehyde (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were labeled by incubation in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (0.1 μg/ml) (Sigma-Aldrich), and samples were mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... in absence or in presence of 50 µM pyridoxal-phosphate-6-azopheny-2’,4’-disulfonate (PPADS) (#P178, Sigma-Aldrich), 100 µM oxidized ATP (OxATP ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated at 37 ℃ and 5% CO2 for 2 weeks and were stained with 500 µl of 4% formaldehyde and 0.005% crystal violet (C3886, Sigma) for 4 hours ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... the cells were mounted on glass slides using Fluoroshield with 4’,6-diamidino-2-phenylindole (DAPI) (F6057; Sigma-Aldrich). Conventional fluorescent imaging was performed using the Axio Oberver.Z1/7 (Carl Zeiss) ...
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bioRxiv - Immunology 2023Quote: ... Serum and supernatant from homogenized tissues were incubated overnight at 4°C with 2% Trichloroacetic acid solution (Sigma, #T0699) at a 1:1 dilution ...
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Glucose) containing 1µg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS and incubated with secondary antibodies for 2 h at RT in 2.5% BSA and 0.1% triton-X in PBS followed by counterstaining with 4’,6-diamidino-2-fenylindool (DAPI) (1µg/µl, Sigma, D9564) for 15 minutes at RT ...
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bioRxiv - Genetics 2023Quote: ... The seminiferous tubules were incubated for 60 min at RT in fixation buffer (100 mM HEPES pH7.4, 4 mM CaCl2, 2.5% glutaraldehyde, 2% PFA, all from Sigma Aldrich) and then the buffer was exchanged with fresh fixation buffer and the samples left overnight fixed at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated for 2 h at 4 °C with 150 μL streptavidin-agarose beads (Sigma-Aldrich) under gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... and pMCAo-21d group received 4 intraperitoneal injections of 50 mg/kg of 5-Chloro-2′-deoxyuridine (CldU, Sigma). The injections were administered 6 ...
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bioRxiv - Microbiology 2022Quote: ... 20 adult female flies (2–4 days old) per vial were fed with 50ug/mL of RU486 (M8046, Sigma; stock solution is 10mg/mL in DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Immunology 2024Quote: ... and the bronchoalveolar lavage (BAL) fluid was collected in 0.5+0.6×4 ml 2% BSA (Sigma, St. Louis, MO). The supernatant of the initial 0.5 ml was stored for cytokine detection ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed four times in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) in 200 ng/mL PBS for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with PBS and mounted using FluoroshieldTM with DAPI (4’,6-diamidino-2-phenylindole) (Sigma, F6057-20ML) (Pinto et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... for 15 min at 4 °C and processed for immunoprecipitation as described previously13,15 using 2 μg PROX1 or control IgG (Sigma) antibody per reaction (Supplementary Table 11) ...
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bioRxiv - Cell Biology 2024Quote: ... Deletion of dynamin proteins in dynamin tKO MEFs were achieved by supplement of 2 μM 4-hydroxytamoxifen (Sigma; H6278) in the GM for 72 days ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed and followed by 3-hour incubation with 0.5 mg/mL MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich). Cell apoptosis was measured with Annexin V Apoptosis Detection Kit I (BD Pharmingen ...
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bioRxiv - Genetics 2024Quote: ... Coverslips were washed twice for 30 min at 37°C with 2x SSC/10% formamide with 0.2 mg/ml DAPI (4’,6-Diamidin-2-phenylindol, Sigma) being added to the second wash ...
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bioRxiv - Cell Biology 2020Quote: ... followed by fixation for 4 min in 1 % formaldehyde (Sigma). Cell pellets were stored at −20 °C or processed immediately.
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-actin clone 4 (EMD Millipore MAB1501, 1:5’000), anti-mouse IgG HRP linked (GE Healthcare NXA931 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-aquaporin 4 (Millipore Sigma; St. Louis, Missouri; 1:200), anti-Pdgfrβ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... anti-aquaporin 4 (Millipore Sigma; St. Louis, Missouri; 1:200), anti-PDGFRβ (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... X10 gels (4:1 molar ratio of dimethylacrylamide (Sigma-Aldrich) and sodium acrylate (Sigma-Aldrich) ...