Labshake search
Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... Seeds were sterilized with (0.05% (v/v) Tween-20 solution for 10 minutes) and subsequently sown on 1/2 Murashige and Skoog (MS) medium (Sigma M5519) at 22□ in long-day conditions (16 h light/8 h dark) ...
-
bioRxiv - Neuroscience 2021Quote: ... or if the number of ORs identified as responsive were more than five standard deviations away from the mean: 1% 2’-hydroxyacetophenone (Sigma H18607).
-
bioRxiv - Developmental Biology 2021Quote: ... DiD staining solution was freshly prepared prior to every assay as a 1:200 dilution of the DiD stock solution [2 mg/mL dilution in N,N-dimethyl formamide (Sigma, #D4551)] in M9 1X buffer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... a control where females were fed a mixture of honey and water (1:2 w/w) or a treatment group fed a mixture of honey and deuterated water (Sigma Aldrich)(1:2 w/w ...
-
Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... Acute deletion of floxed A20 and Abin-1 exons in vivo by oral gavage was performed using tamoxifen (2 mg/day, Sigma, T5648) for 3 consecutive days as indicated for survival analysis ...
-
bioRxiv - Neuroscience 2020Quote: ... and blocked with 2% donkey serum and then immunostained using rabbit antibody against Cx43 (Figure 6A, Sigma, cat# C6219, 1:500) or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2 ...
-
bioRxiv - Immunology 2020Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/mL-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich,) to the cell surface ...
-
bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
-
bioRxiv - Immunology 2021Quote: ... The only two variations from the written protocol were (1) an open wash dispense after the peroxide step and (2) DAPI (Sigma D9542) was dispensed twice at the end of the protocol at a concentration of 1 µg/mL ...
-
bioRxiv - Genomics 2021Quote: ... the supernatant was removed by aspiration and the nuclear pellet was resuspended in 200 uL staining mix (2% normal goat serum, 0.1% BSA, 1:500 anti-NeuN conjugated to AlexaFluor488 (Millipore, cat#: MAB377X) in PBS ...
-
bioRxiv - Cell Biology 2021Quote: Mission plasmids directing expression of shRNAs targeting PlexinA2 (TRCN0000061499 (ShPlexA2#1) and TRCN0000061501 (ShPlexA2#2)) or PlexinA4 (TRCN0000078683) were purchased from Sigma Aldrich. The production of the lentiviruses ...
-
bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
-
bioRxiv - Microbiology 2023Quote: ... the respective drugs were serially diluted (1 in 2) in liquid YPD with the highest concentrations for myriocin (Sigma Aldrich, M1177) at 128 μg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... albicans yeast at the ratio of 1:20 in RPMI 1640 medium containing 2% heat-inactivated mouse serum (#S3509, Sigma-Aldrich) at 37°C for 3 h.
-
bioRxiv - Neuroscience 2023Quote: ... Embryos and larvae were raised until 5 days post fertilization (dpf) in E3 medium containing methylene blue or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
-
bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
-
bioRxiv - Cell Biology 2023Quote: ... The washed nuclei pellets were resuspended in sorting buffer (2% BSA/RNase-free PBS) and labeled with anti-PCM1 (1:500, HPA023374, Sigma-Aldrich) on ice for 45 minutes ...
-
bioRxiv - Microbiology 2023Quote: Synechococcus WH7803 wild type and mutant cultures were grown in constant light at 12 μmol quanta m−2 s−1 in Pro99 media amended with high molecular weight chitosan or colloidal chitin (Millipore Sigma) to a final concentration of 56 μg/ml in triplicates ...
-
bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
-
bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
-
bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
-
Complement receptor C5aR1 signaling in sensory neuron-associated macrophages drives neuropathic painbioRxiv - Pharmacology and Toxicology 2022Quote: ... or PSNL mice and incubated in a solution containing 1 ml of DMEM medium and 2 mg/ml of collagenase type II (Sigma-Aldrich) for a 1 h at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
-
bioRxiv - Neuroscience 2024Quote: ... 1×10^6 POE SHSY5Ys were reverse transduced with the 1:2 lentivirus supernatant dilution in the presence of 10 µg/ml polybrene (Sigma Aldrich). Bulk populations of cells stably expressing mitoSRAI were established through selection with 1ug/ml puromycin (M P BIOMEDICALS UK) ...
-
bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... barcode set in suspension using identical concentrations for 2 hours at room temperature before 2x washes with GSH-CSB (1 mM Gulutathione (Sigma #G6529)) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
-
bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
-
bioRxiv - Microbiology 2024Quote: ... Donor and recipient cells were mixed to a ratio of 10:1 and 2 µL of the mating was spot into a microscope slide containing an M9 (M9 salts (Sigma-Aldrich), 500M mM MgSO4 ...
-
Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... samples were cleared using a 1:2 mixture of Benzyl Alcohol: Benzyl Benzoate (108006, B6630, Sigma-Aldrich, St. Louis, MO, USA).
-
bioRxiv - Developmental Biology 2023Quote: ... samples were dehydrated in series of Methanol and cleared using BABB (a mixture of 1 part benzyl alcohol to 2 parts benzyl benzoate; Sigma-Aldrich, benzyl alcohol ...
-
bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
-
bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
-
bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore, Burlington ...
-
bioRxiv - Neuroscience 2023Quote: ... A high-titre solution of viral vector (AAV9-syn-jGCaMP7f-WPRE) was diluted to a final ratio of 2:1 with a 20% mannitol solution (Sigma-Aldrich) to promote diffusion ...
-
bioRxiv - Neuroscience 2024Quote: ... the dehydrated tissue was immersed in a BABB solution (benzyl alcohol and benzyl benzoate in a 1:2 ratio; Sigma Aldrich). The cleared ganglia were subsequently mounted between a slide glass and a coverslip ...
-
bioRxiv - Cell Biology 2024Quote: ... mucus from injected animals was removed by cooling worms on ice for 1 min then incubating on ice-cold 2% HCl (Sigma H1758) for 3 min ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by the addition of 1 mg/mL MTT reagent (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich, Darmstadt, Germany). After the incubation period of 4 h ...
-
bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% BoltTM Bis-Tris gels (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were mounted in 3D homemade printed chambers covered with coverslips and sealed with a melted paraffin–vaseline mixture (∼2:1 ratio) (Cat. #: P3558, Sigma-Aldrich. Vaseline pure ...
-
bioRxiv - Cancer Biology 2023Quote: ... Stable KD of EIF4G2 was generated by infecting HEC-1A or RL95-2 cells with lentiviruses harboring pLKO.1-puro plasmid expressing shRNA targeting GFP (Control) or shRNA targeting EIF4G2 (Sigma TRCN0000147914), followed by selection using puromycin ...
-
bioRxiv - Developmental Biology 2024Quote: ... was performed by incubating the zebrafish for 24 hours with 1 mM (2-month-old fish) or 10 mM (larvae) MTZ (Sigma-Aldrich) diluted in 1% DMSO (VWR ...
-
bioRxiv - Molecular Biology 2023Quote: ... the arrested culture was split into three subcultures: one subculture was treated with phleomycin (2-10 μg·mL−1; Sigma-Aldrich, P9564), a second one with β-estradiol (2 μM ...