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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... β-(1–4) glycans were stained with calcofluor white (Sigma) as described (10 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 0.75□×□10−4 M 1-Thiolglycerol (MTG, Sigma), Penicillin/Streptomycin (P/S ...
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bioRxiv - Cancer Biology 2022Quote: ... activated (Sigma-Aldrich, clone HUTS-4, Cat. #: MAB2079Z, 1:400), Integrin β1 (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 4 × 107 cells were fixed using 1% formaldehyde (Sigma-Aldrich) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ESET deletion was induced by 1 μM 4-OHT (Sigma) treatment in ESET cKO cells ...
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bioRxiv - Neuroscience 2022Quote: ... probed with anti-chondroitin-4-sulfate (1:1000; Millipore: MAB2030) or anti-chondroitin-6-sulfate (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 3-isobutyl-1-methylxanthine (0.5 mM; Sigma, 28822-58-4), indomethacin (200 μM ...
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bioRxiv - Cell Biology 2020Quote: ... 1 % Triton-X and 1x BugBuster (Merck Millipore, #70584-4) were added to lyse the bacteria for 30 min at 4 °C with gentle rotation ...
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bioRxiv - Neuroscience 2023Quote: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (Sigma or MedChemExpress) was administered according to a 5 × 20mg/kg dosing paradigm ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 µM 1-Oleoyl lysophosphatidic acid sodium (LPA; Sigma, L7260), 10 ng/ml recombinant human LIF and 10 μΜ Y27632 ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µM 1-Methyl-4-phenylpyridinium iodide (D048, MPP+, Sigma) was applied to induce cell death over 60 hours ...
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bioRxiv - Neuroscience 2024Quote: ... carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP; 1 μM, Sigma-Aldrich), the uncoupler ...
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bioRxiv - Microbiology 2023Quote: ... Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) (1 μM; Sigma, C2920), Rotenone (0.5 μM ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 4 mg mL−1 lysozyme (Sigma cat # L6876) was added to each pellet ...
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bioRxiv - Physiology 2022Quote: ... 300 μL of 4:1 Free Glycerol Reagent:Triglyceride Reagent (Sigma) was added to each well and the plate was incubated at 37 °C for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-rabbit Aquaporin 4 (1:250) from Sigma (AB3594) were used ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μL MSTFA 1% TMCS (24589-78-4, Sigma-Aldrich) were added and the second derivatization was performed for 1 h at 70°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sub-cutaneous injections of flunixin-meglumine (4 mg·kg-1, Sigma) were applied for analgesia after the surgery ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 4 μg.ml−1 polybrene (#H-9268, Sigma Aldrich) and 1/10 (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Biophysics 2021Quote: HeLa H2B-mCherry day 2 aggregates were equilibrated for 1 h in CO2 independent medium containing 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... two CSNK1D targeting shRNA constructs were selected from the Mission TRC shRNA library (TRCN0000023769, named shCSNK1D#1; TRCN0000361946, named shCSNK1D#2; Sigma-Aldrich); non-targeting scrambled shRNA served as control (shc002 ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to lysis in ChIP lysis buffer (20 mM Tris-HCl, 150 mM NaCl2, 2 mM EDTA, 1% IGEPAL CA-630 (Sigma-Aldrich) and 0.3% SDS in water) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... fixed cells were permeabilized with PBS containing 0.2% Triton X-100 for 10 min and blocked with PBS containing 1% IgG-free bovine serum albumin (BSA) (Sigma, A2058) for 30 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... hiPSC-CMs were maintained in RPMI 1640 with HEPES and Glutamax (Life Tech, Bleiswijk, the Netherlands) with 2% B27 with insulin (Life Tech) and 1% penicillin/streptomycin (P/S, Sigma-Aldrich). Six days before mechanical stimulation ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue is then washed twice with 2-3 mL sterile-filtered DM+Trypsin Inhibitor (1 mg/mL; Sigma Aldrich, T9253) and incubated in the 3rd wash for 3-5 min at 37° C ...
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bioRxiv - Neuroscience 2021Quote: ... A crude nuclei suspension was prepared from the homogenate by 1-2 rounds of washing (Nuclei PURE store buffer, Sigma, S9183) and centrifugation (700g for 3 minutes at 4 degrees) ...
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bioRxiv - Molecular Biology 2020Quote: ... the cryosections were incubated with Alexa FluorTM 488 goat-anti-Rabbit IgG (1:1000) for 2 hours at room temperature and then the nuclei counterstained with Hoechst 33342 (Sigma-Aldrich). The cryosections were imaged using the A1+ Confocal Microscope System with NIS-Elements Advanced Research software (Nikon ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was gradually dehydrated in resistant glassware with incremental concentrations of tetrahydrofuran (TFH, 50%, overnight; 80%, 1 h and 100%, 2 h, v/v % in distilled water, Sigma-Aldrich). Incubations were performed on a shaker at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... A refractive index matching solution was prepared by mixing BABB (benzyl alcohol + benzyl benzoate 1:2; Sigma-Aldrich, 24122 and W213802) and DPE (diphenyl ether ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... Osteogenic induction was performed in low-glucose DMEM medium supplemented with 50 μg mL−1 L-ascorbic acid 2-phosphate (Sigma-Aldrich), 10 mM glycerol 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... diluted in PBS for 2 h at room temperature and counterstained with 1:5000 Hoestch solution (bisBenzimide H 33258, Sigma-Aldrich). Sections were washed for 20 min in PBS (4 x 5 min rinses) ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2020Quote: ... were homogenized with 1 ml H20 and extracted by vortexing with 2 ml of chloroform/methanol 2:1 (v/v) and 10 μl of synthetic internal standard (DAG 15:0/15:0, Sigma Aldrich), sonicated for 30 s ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fabricated with a final concentration of 1×105 AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO2 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The remaining lysate was then incubated with 25 μL (1 μL/2 OD600 nm of cells) of monoclonal anti-FLAG M2 (Sigma #F1804) at 4°C with rotation for 30 min ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... Fibroblasts were cultured in DMEM or tumour cell conditioned media supplemented with 2% FBS and 50 μg mL−1 ascorbic acid ascorbic acid (Sigma, A4403) on gelatin-coated plates ...
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (bovine serum albumin; Sigma Aldrich, Tween20, PBS) and subsequently incubated in primary antibody (cytokeratin 18 ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (bovine serum albumin; Sigma Aldrich, Tween20, PBS) and subsequently incubated in primary antibody (cytokeratin 18 ...