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Citations for Millipore Sigma :
3601 - 3650 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Whole-mount immunostained embryos were mounted either in 1:2 Benzyl Alcohol:Benzyl Benzoate (BABB, Sigma-Aldrich cat# B6630 and 305197) clearing mixture or Vectashield (VectorLabs ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.3% NP-40) containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA), followed by clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Cancer Biology 2023Quote: ... Clonal cell lines were selected by pre-determined concentration of puromycin (0.5, 1 and 2 µg/ml; Sigma, Cat# P8833) for respective cell line and overexpression of desired protein was confirmed by Western blotting.
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bioRxiv - Neuroscience 2023Quote: ... larvae were serially dehydrated in 50% methanol and 100% methanol for clearing with BABB (Benzyl alcohol:benzyl benzoate 1:2, Sigma-Aldrich) for 24 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: Aliquots from washed and equilibrated (in 1 x degradation buffer) ADP-ribosylated (1 μl) and RNAylated (2 μl) rS1 (32P) were treated with 0.5 U endonuclease P1 (Sigma-Aldrich) 4 or 0.95 μM ARH1 or ARH3 (human ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each sample was lysed in 500 μL of lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, 1x protease inhibitor cocktail (Sigma-Aldrich)) for 20 mins at RT before heating to 99 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated on ice for approximately 1 h before the addition of Amberlite XAD-2 beads (Sigma-Aldrich). The reconstitution mixture was incubated at 4°C overnight on an orbital shaker.
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bioRxiv - Cell Biology 2023Quote: ... permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... [Ca2+]i responses to 80 mM KCl and 2) immunofluorescence in cells stained with smooth muscle-specific a-actin (SMA; 1:400, A2547, Sigma) and calponin (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellet were lysed in 500 µL of freshly prepared lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF and protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Biophysics 2023Quote: ... The cell drop was then immersed in 2 mL Leibovitz L15 -/-medium containing 1 μL/mL Hoechst dye (Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Pathology 2024Quote: ... The nematodes (100 worms/100 μl) were treated for 2 h in 1.5 ml tubes with 0-1 mM ascorbic acid (Sigma-Aldrich), 0-200 μM doxycycline hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The infectious bloodmeal consisted of a 2:1 mix of washed rabbit erythrocytes (BCL) supplemented with 10 mM adenosine triphosphate (Sigma) and viral suspension supplemented with Leibovitz’s L-15 medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in PBS with 2% HI-FBS (Hyclone, #SH30910.03) containing 1 µg/mL DAPI (Sigma Aldrich, #D9542) to detect dead cells ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor nodules from each mouse were diced with scissors then incubated for one hour at 37 °C in 2 mL of 1 mg/mL collagenase type IV (Sigma) in RPMI media ...
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bioRxiv - Immunology 2024Quote: ... coli at MOI of 100 were added for 2 h to 1×105 neutrophils seeded onto 0.01% poly-l-lysine (Sigma-Aldrich) treated 96-well black cell culture plates with the optical bottom ...
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bioRxiv - Immunology 2024Quote: ... The samples were blocked for 1 h at room temperature in PBS containing 2% bovine serum albumin (BSA, Sigma-Aldrich). After additional PBS washing ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in digestion medium (RPMI supplemented with 2% iHS [Sigma], 1 mg/ml Collagenase XI [Sigma], and 10 U/ml DNase I [Sigma]). Following digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... The slices were diced into 1 mm pieces and then treated with 10 mM tris(2-carboxyethyl)phosphine hydrochloride (SIGMA) at 56°C for 30 minutes for reduction ...
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bioRxiv - Cell Biology 2024Quote: ... each chamber contains 2 mL of respiration buffer (90 mM KCl [P4504, Sigma], 50 mM MOPS [M1254, Sigma], 1 mM EGTA [E4378, Sigma] ...
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bioRxiv - Microbiology 2024Quote: ... were determined using the standard microbroth dilution protocol68 and interpreted using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines.1 Cells were grown in Mueller Hinton Broth 2 (MHB2) (Millipore) and the inoculum size was set to 5 x 105 bacteria per ml ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were incubated ON at 4°C with primary antibodies in 5% serum and 0.1% Saponin in PBS: Mouse anti-microtubule-associated protein 2 (MAP2)ab 1:2000 (Sigma, M1406); rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2024Quote: ... the arrested culture was split into two subcultures: one subculture was treated with phleomycin (2 µg·mL-1; Sigma-Aldrich, P9564), and the one was left untreated (mock) ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated with anti-SARS-CoV-2 Nucleoprotein primary antibody (CTAD Mount Sinai #NP-1C7) in 1% bovine serum albumin (Sigma) and 0.5% TritonX-100 at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... followed by the addition of norharmane matrix (10mg/mL in 1:2 MeOH : CHCl3 – Sigma Aldrich, St. Louis, MO, USA). Mass spectra were collected in negative-ion mode using a Bruker timsTOF Flex ...
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bioRxiv - Neuroscience 2024Quote: ... bacteria were grown in isotope-enriched M9 minimal medium containing 1 g/L of [15N]ammonium chloride and 2 g/L of [13C]glucose (Sigma), supplemented with 1.7 g/L yeast nitrogen base (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Inducible shRNA-mediated knockdown as well as iNgn1/2 cell lines were treated with 1 µg/ml doxycycline (Sigma Aldrich).
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bioRxiv - Immunology 2024Quote: ... lungs were minced and transferred to 50 mL tubes containing 2 mL of digestion buffer (1 mg/mL type IV collagenase and 0.1 mg/mL DNase [Sigma-Aldrich] in Dulbecco’s PBS [DPBS] with calcium and magnesium) ...
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bioRxiv - Cancer Biology 2024Quote: ... xenograft tumors excised from mice were weighed before immediate mechanical and enzymatic dissociation in DMEM-collagenase media for 1 hour in a 37°C shaker at 150 rpm (Hyclone DMEM, 2 mg/mL Collagenase type IV (Sigma), 5% FBS (Atlas Biologicals) ...
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bioRxiv - Plant Biology 2024Quote: ... Extraction buffer (50 mM Tris-HCl pH 7.2, 2% Triton X-100, 1% plant protease inhibitor cocktail P9599 from Sigma Aldrich) was added to the homogenized material and subjected to sonication for 15 min using an ultrasound bath (Sonorex RK52 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9 were delivered by a single injection to the right lateral ventricle at a dose of 1×1011 or 2×1011 genome copies per animal at P1 in a PBS solution containing FastGreen dye (Sigma) as previously described.23
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bioRxiv - Neuroscience 2024Quote: ... and 0.33 mM MgSO4) containing 0.01 % methylene blue to suppress fungal growth or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
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bioRxiv - Cancer Biology 2024Quote: ... The colonic tissue was then immersed in falcon tubes containing wash buffer (2% BSA, 100 U ml−1 penicillin–streptomycin (P0781 Sigma) and 5 mM EDTA in HBSS ...
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bioRxiv - Systems Biology 2024Quote: ... followed by incubation with primary antibodies (Shroom-3 LS-Bio, #LS-C679459,1:100; Fluorescein LTL, Vector lab-#FL1321-2, 1:200; FLAG, Millipore-Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... The lymphatic networks in various regions of the terminal ileum were perfused with Krebs containing 1-2% FITC-dextran (Sigma) using sharpened micropipettes (∼40 um tip diameter) ...
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bioRxiv - Bioengineering 2024Quote: ... 2:1) were dissolved until homogeneous in a 10 wt% solution containing acetic acid (90% aqueous solution; Sigma Aldrich A6283), citric acid (15 wt% based on gelatin weight ...
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bioRxiv - Developmental Biology 2024Quote: ... Pooled tissues were then transferred to a clean watch glass containing 200 µL of a 1:2 mixture of accutase (Sigma) and 0.25% trypsin in PBS and incubated 15 min at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... followed by incubation with primary antibodies (Shroom-3 LS-Bio, #LS-C679459,1:100; Fluorescein LTL, Vector lab-#FL1321-2, 1:200; FLAG, Millipore-Sigma, #F1804,1:400 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM tris(2-carboxyethyl)phosphine (TCEP)) with the addition of 10 µg/mL DNase I (bovine pancrease, Sigma-Aldrich), 100 µg/mL lysozyme (chicken egg white ...
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bioRxiv - Genomics 2024Quote: ... The biopsy was minced into 1-2 mm3 pieces and digested with 0.7 mg/ml type 1A collagenase (Sigma C9891) in culture media at 37°C for 45 min with gentle agitation every 15 min ...