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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos older than 24 hpf were transferred into E3 medium containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma-Aldrich P7629). Animal work was approved by the local Animal Welfare and Ethical Review Body (AWERB ...
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bioRxiv - Biophysics 2023Quote: ... The protein was eluted at 1.5 ml/min using the following gradient- 0 - 1 minute: 10% buffer 2 (filtered Acetonitrile (Sigma-Aldrich) with 0.1% TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... Penis and lymph nodes were dissected and fixed for 1.5-2 h in 3% paraformaldehyde (penis) or overnight in 1% paraformaldehyde (PFA, Sigma, P6148) at 4°C (lymph nodes ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were concentrated to 2 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 300-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Immunology 2023Quote: ... B cells were suspended at 2×106 cells per milliliter and rested in RPMI medium containing 1% low-lipid BSA (SRE0098, Sigma) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... were obtained by PCR with the primers P1-P2 and P2-P4 respectively (Supplementary Table 2) and cloned into pETDuet-1 (Novagen) using the In-Fusion HD Cloning system (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... An equal volume of chilled FACS buffer (0.2 μm filter-sterilized 1x PBS, 2%(v/v) FBE (fetal bovine essence, Avantor),1% penicillin (Sigma, P7794), 1% streptomycin (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the tissue is first allowed to fold into a spheroid in a regular HM and then transferred to the experimental setup embedding it in a soft-gel (1%-2% low-melting-point agarose-Sigma) prepared in HM and cooled down before the introduction of the sample to prevent damage of the tissue ...
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bioRxiv - Microbiology 2023Quote: ... followed by the seeding of synchronized Pf3D7 ring stage culture at 1% parasitemia and 2% hematocrit in standard RPMI 1640 (Sigma)-based medium ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed three times in RPMI + 10% FBS and resuspended at 2 × 107 cells/ml in PBS supplemented with 0.4 μg ml−1 LPS (Sigma-Aldrich). DCs were injected (5 × 105 cells in 25 μl ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Neuroscience 2022Quote: ... peptides were acidified with 2 μl of 1% TFA then desalted using C18 ZipTips® (Millipore, cat no. Z720070-96EA).
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... All zygotes were collected from oviducts 24 h after the human chorionic gonadotropin injection and were then freed from any remaining cumulus cells by a 1-2 min treatment of 0.1% hyaluronidase (Sigma-Aldrich) dissolved in M2 medium (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were blocked for 1 hour with blocking buffer (Cyto-TBS + 2% bovine serum albumin (BSA, Sigma Aldrich, 1002695029)) and incubated with a primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... XAV939 (1 μM, Stemgent, 04-00046) and LDN-193189 (50 nM, Stemgent, 04-0074) with doxycycline hyclate (2 μg.mL−1, Sigma, D9891) and Zeocin (1 μg.mL−1 ...
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bioRxiv - Neuroscience 2022Quote: ... Stemgent, 04-00046) and LDN-193189 (100 nM, Stemgent, 04-0074) along with doxycycline hyclate (2 μg.mL−1, Sigma, D9891) with Y27632 (5 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and pelleted before being suspended in a formaldehyde-reversal lysis buffer containing 500mM Tris + 150mM NaCl + 1% Triton X-100 + 2% SDS + 50mM 1,4-dithiothreitol (DTT) + 1X cOmplete EDTA-free Protease inhibitor cocktail (Sigma-Aldrich), pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Goat anti-ChAT (1:200, Millpore) diluted in PBS supplemented with 2% horse serum and 0.1% triton X-100 (Sigma-Aldrich) were applied onto the sections followed by overnight incubation at 4°C in a humidified chamber ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of as-synthesized SWCNT powder was added to 2 mg of (GT)xx oligonucleotide (Integrated DNA Technologies) 1 mL of 0.1 M NaCl (Sigma-Aldrich). Each sample was ultrasonicated using a 1/8′′ tapered microtip for 30 min at 40% amplitude in an ice bath (Sonics Vibracell VCX-130 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed with PBS and permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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bioRxiv - Neuroscience 2024Quote: ... media was changed once per week with the addition of 1 μM 5-Fluoro-2′-deoxyuridine (Cat.#F0503, Sigma-Aldrich) and 1 μM Uridine (Cat.#U3003 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were re suspended in FACS buffer (0.1% BSA in PBS and 2% FBS) containing 50µg/ml of DNase 1 (Sigma D-4513) to reduce clustering of cell samples ...
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bioRxiv - Microbiology 2024Quote: ... Activated macrophages were inoculated with Leptospira at a multiplicity of infection (MOI) of 100 bacteria-per-cell during 2 hr following by 1 hr of gentamicin treatment (Sigma) at 100 µg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... with 1% fetal bovine serum (FBS) and 100 U/ml penicillin/streptomycin supplemented with 2 mg/ml collagenase (SCR103, Sigma) and 10 U/ml DNAse (D4263 ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells Glucose (10 mM) was first injected followed by Oligomycin (1 µM) then 2-Deoxy-D-glucose at 10 mM (Sigma). At least 30 wells were assayed for each cell type ...
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bioRxiv - Cell Biology 2024Quote: ... 1x Insulin-Transferrin-Selenium-A and 2 mM L-glutamine] supplemented with 1 μg/mL doxycycline (DOX; Sigma-Aldrich, # D9891), 10 μM SB431542 and 0.1 μM LDN193189 to promote a cortical fate ...
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bioRxiv - Cell Biology 2024Quote: ... extracts were produced as in70 without modifications and co-immunoprecipitation was performed by incubating 1-2 mg of nuclear extract with pre-equilibrated anti-HA Affinity Matrix (50 μl, Sigma) or agarose GFP traps (30 μl ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Plant Biology 2024Quote: Auxin treatments were performed by immersing dissected SAM in 1 or 2 mM Indole-3-acetic acid (IAA) solution (Sigma) for five hours as described (Galvan-Ampudia et al. ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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bioRxiv - Cell Biology 2023Quote: ... [Ca2+]i responses to 80 mM KCl and 2) immunofluorescence in cells stained with smooth muscle-specific a-actin (SMA; 1:400, A2547, Sigma) and calponin (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellet were lysed in 500 µL of freshly prepared lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF and protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were grown at 28°C in E3 medium containing 1-phenyl-2-thiourea (0.003%, PTU, Sigma-Aldrich, P7629-10,) to avoid pigmentation ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Biophysics 2023Quote: ... The cell drop was then immersed in 2 mL Leibovitz L15 -/-medium containing 1 μL/mL Hoechst dye (Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Microbiology 2022Quote: ... was diluted to 1 μg/mL in 1% goat serum in PBS with 100 ng/mL of 4’6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 60 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... at 58°C for 2 hr post fixation and subsequently immersed in 1% phosphomolybdic acid (Sigma, St Louis, MO, USA) and 0.5% phosphotungstic acid solution (Sigma ...
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bioRxiv - Genomics 2022Quote: ... Induction of DUX4 and SRSF3 transgenes was achieved by culturing cells in 1-2 μg/mL doxycycline hyclate (Sigma-Aldrich). Differentiation of myoblasts into myotubes was achieved by switching the fully confluent myoblast monolayer into DMEM containing 1% horse serum (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was transferred to a fresh tube and reduced with 1 mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 minutes at 60°C and alkylated in 4 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (2 × 15 cm plates) were incubated for 24 hours in complete media supplemented with 1 μg/ml tetracycline (Sigma) prior to harvesting similar to the BioID group.