Labshake search
Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
-
bioRxiv - Plant Biology 2021Quote: SDS washed and de-starched dry onion peels were weighed and added to a 2 mL screw – top microcentrifuge tube and 1 mL of 3 M HCl in methanol (Sigma, USA) was added to the walls and incubate for 16 h at 80°C in heating block in a fume hood as per De Ruiter et al ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with 5% skimmed milk for 1 hour at room temperature and then with primary antibodies diluted in 2% bovine serum albumin (Sigma-Aldrich) in tris-buffered saline 0.05% Tween 20 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: HAE mucus was allowed to accumulate for one week prior to the application of 5 μl of 2 μm red-fluorescent (Cy3) polystyrene microspheres (1:50 dilution, Sigma-Aldrich) to the apical chamber of the transwell ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
-
bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
-
bioRxiv - Physiology 2020Quote: ... Frozen tissue was sectioned into 2 mm coronal sections and a 1 mm diameter biopsy tool (Harris Micro-punch, Millipore-Sigma, Z708658) was used to collect tissue punches in ipsilateral regions distal and proximal to the infarcted tissue similar to IHC regions (Figure 3B) ...
-
bioRxiv - Microbiology 2021Quote: ... Two-fold serial dilutions of an initial concentration of 5 μg/ml of the mAbs were incubated with NDV LaSota or rNDV-H5 for 3h at 37°C in complete medium supplemented with 50 ng/ml L-1-tosylamido-2-phenylethylchloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich). Subsequently ...
-
bioRxiv - Microbiology 2020Quote: ... for 45 minutes followed by 2 washes in a PBS buffer containing 1% FBS and 0.5% Saponin (Sigma, St Louis, MO). Intracellular staining for anti-IFNγ (2µg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... Each strain was grown in glucose-yeast extract-peptone (GYEP) liquid medium (0.3% yeast extract and 1% peptone; Difco, Soria Melguizo) with 2% glucose (Sigma-Aldrich, Spain) for 24 h to 48 h at 30°C ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
-
bioRxiv - Cancer Biology 2021Quote: ... specific siRNAs (si-HER3-1: 5’-GCUGAGAACCAAUACCAGA, si-HER3-2: 5’-CCAAGGGCCCAAUCUACAA) and a validated control siRNA were obtained from Sigma-Aldrich.
-
bioRxiv - Cell Biology 2021Quote: ... subcellular fractionation was performed on the remaining cells by suspending them in 10 ml cold hypotonic buffer (10 mM Tris pH 7.5, 10 mM KCl, 1 mM MgCl2, 10 mM glycerol 2-phosphate (Sigma-Aldrich #G9422), 1 mM EDTA ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... All embryos of 26 hpf or older were raised in fish water containing 200 μM 1-Phenyl-2-thiourea (PTU; Sigma Aldrich) to prevent melanin synthesis ...
-
bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
-
bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
-
bioRxiv - Cell Biology 2022Quote: ... [Ca2+]i responses to 80 mM KCl and 2) immunofluorescence in cells stained with smooth muscle-specific α-actin (SMA; 1:400, A2547, Sigma), calponin (1:100 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... HeLa cells subjected to Dox-induced expression of the PAR-T sensor proteins were treated with 20 μM PJ34 or 20 μM PDD 00017273 for 2 hours prior to induction of DNA damage by treatment with H2O2 (1 mM; Sigma, 216763) for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
-
bioRxiv - Developmental Biology 2022Quote: ... (1 mg/ml) and incubated in 50 μl droplets of 10 µM 2′,7′- dichlorodihydro-fluorescein diacetate (H2DCFDA, D6883, Sigma-Aldrich) in PVA-PBS for 15 min at 37 °C in an atmosphere of 5% CO2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation cancer cells (in the indicated cell numbers) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female (for breast cancer xenograft ...
-
bioRxiv - Biophysics 2022Quote: ... The tyrosinated tubulin level was detected by western blot using monoclonal anti-tyrosinated tubulin antibody (clone YL1/2; 1:2500 dilution; EMD Millipore) as primary antibody and a goat anti-rat secondary antibody (alkaline phosphatase-conjugated ...
-
bioRxiv - Genetics 2022Quote: ... The pellet was resuspended in 1x PBS and cells were stained with a solution containing 1 µg/ml 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich India) for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were transferred to BABB solution (1:2 ratio of benzyl alcohol, Sigma- Aldrich, 305197; and benzyl benzoate, Sigma-Aldrich, B6630) for at least 3 hr ...
-
bioRxiv - Immunology 2021Quote: The right caudal lope of the lung was removed from the body and transferred on ice in PBS containing 1% BSA (w/v) and 2 mg/ml Actinomycin D (Sigma-Aldrich) for further processing ...
-
bioRxiv - Microbiology 2021Quote: Freshly purified sporozoites (~10-50 million) were suspended in 1 ml of primary fix (2% freshly-prepared formaldehyde solution (Sigma-Aldrich), 2.5% electron microscopy grade glutaraldehyde (TAAB ...
-
bioRxiv - Neuroscience 2021Quote: ... Three rats were subcutaneously injected with normal saline (Day 1, NaCl, 0.9% 10 mg/Kg) and D-SER (Day 2, 10 mg/Kg, Sigma-Aldrich Ireland) and placed in a box (24h x 38w x 38l cm) ...
-
bioRxiv - Plant Biology 2021Quote: ... Then the seedlings were transferred into 1/2 MS liquid medium contained 50 μM CHX and 50 μM BFA (Sigma-Aldrich) for 2 hours ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
-
bioRxiv - Physiology 2020Quote: ... we also conducted 2 enzymatic lysis steps consisting of lysis solution 1 (50 mg/mL lysozyme and 20% RNase – Sigma R6148) at 37°C for 1 hour and lysis solution 2 (25 µL of 25% SDS ...
-
bioRxiv - Immunology 2021Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/ml-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich) to the cell surface ...
-
bioRxiv - Neuroscience 2022Quote: ... for 2 h at room temperature and finally counterstained for 1 min with Hoechst 33258 (10 μg/mL in PBS, Sigma-Aldrich). Images were taken using a confocal microscope LSM-800 Zeiss (28148) ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with protease and phosphatase inhibitors (1 tablet per 5ml buffer of cOmplete™, mini, EDTA-free protease inhibitor cocktail [Roche], 1:100 phosphatase inhibitor cocktail 2 [Sigma] ...
-
bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore; 1:300 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids containing shRNAs targeting STING (shSTING-1 #TRCN0000161345, shSTING-2 #TRCN0000163029) or non-targeted (scrambled) shRNA (shNT #SHC016-1EA) were purchased from Sigma Aldrich. STING-IRES-GFP and DN-IRES-GFP lentiviral plasmids were generated by inserting the cDNA of STING or STING-DN ...
-
bioRxiv - Paleontology 2020Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore, Burlington ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The loading solution consisted of 15 µL of the protein sample mixed with 1 µL DTT and 15 µL Laemmli 2× Concentrate Loading Buffer (Sigma-Aldrich), and denatured at 95 °C for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
-
bioRxiv - Microbiology 2021Quote: TREx-BCBL-1-RTA cells were seeded onto poly-L-lysine coated coverslips and 2 µg/mL doxycycline hyclate (Sigma-Aldrich) was added 3 hours later ...
-
bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
-
bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
-
bioRxiv - Cell Biology 2021Quote: ... we made 200 μl of gelation solution by mixing 188 μl of monomer stock solution (1 × PBS, 2 M NaCl, 8.625% (w/w) sodium acrylate (SA)(Sigma Aldrich 408220), 2.5% (w/w ...
-
bioRxiv - Physiology 2021Quote: ... Then centrifuged for 5 minutes at 800 g at 5°C and the pellet was resuspended in 500 μl sorting buffer (1 % BSA wt/vol, 2 % skimmed milk powder (Sigma Aldrich), 1 mM EDTA in PBS pH 7.4 ...
-
bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
-
bioRxiv - Immunology 2021Quote: ... Eggs were obtained by natural spawning and fertilized embryos were treated with 0.2mM 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich, P7629) to prevent melanophore formation ...
-
bioRxiv - Developmental Biology 2021Quote: ... The samples were cut into small pieces and digested in 2 ml of digestion mix: 2mg/ml collagenase type 1 (#SCR103; EMD Millipore) in DMEM-F12 supplemented with 5% FBS (#10438026 ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed twice in 0.1M PBS and blocked for 2 hours with 0.1M PBS containing 1% bovine serum albumin and Triton X-100 (0.1%) (both from Sigma Aldrich, Gilligham, UK) and then incubated for 48 hours at 4°C with the primary antibody ...
-
bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and precipitated in 2.5 volumes of 100% ethanol with 2 μL glycogen (Sigma Aldrich 10901393001). Pellet was washed once in 1 mL 100% ethanol and dissolved in 40 μL 10 mM Tris-HCl pH 8.5 ...