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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The lymphatic networks in various regions of the terminal ileum were perfused with Krebs containing 1-2% FITC-dextran (Sigma) using sharpened micropipettes (∼40 um tip diameter) ...
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bioRxiv - Cell Biology 2024Quote: Five- to 7-day-old female mosquitoes were deprived of 10% sucrose solution 18-24 hours before exposure to an artificial bloodmeal consisting of a 2:1 mix of washed rabbit erythrocytes (BCL) supplemented with 10 mM adenosine triphosphate (Sigma) and Leibovitz’s L-15 medium (Gibco ...
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bioRxiv - Biochemistry 2024Quote: 8.500 cybrids/cm2 were plated on Aclair foils (Plano) and after 24 hours were fixed for 1 hour with pre-warmed 2% glutaraldehyde (Sigma), 2.5% sucrose ...
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bioRxiv - Biochemistry 2024Quote: ... and 20 µL of the sample was taken at various time points (0, 15, and 30 minutes) and immediately quenched with 2 µL of 1 M ammonium acetate (Sigma). Quenched samples were diluted with 4X Laemmli gel loading buffer to a final volume of 30 µL ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular proliferation rates were determined beginning 1 day after seeding with media containing 2 μg/ml or the indicated amount of doxycycline (Sigma) or without doxycycline (for controls ...
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bioRxiv - Genetics 2024Quote: ... for 1 h followed by 2 h incubation at room temperature with primary antibodies and 1 h with secondary antibodies and DAPI (Sigma) staining ...
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bioRxiv - Cell Biology 2024Quote: ... two siRNA sequences targeting different regions of NOK were designed as #1: 5’-GCAAGAAACAUUCAUGCAU-3’ and #2: 5’-GUCUUUCCCAGGGACACAA-3’ (SASI_Hs01_00043410 and SASI_Hs02_00351937, Sigma, St. Louis, MO, USA). The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... either 4 mg of Tamoxifen or 1 mg of 4-OH Tamoxifen (Sigma-Aldrich, H6278) was delivered on day e9.5 or e10.5 (Fig ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis of splenocytes was induced by incubation with 4-nitroquinoline 1-oxide (4-NQO, Sigma) for 18 hours as we described previously (25) ...
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bioRxiv - Neuroscience 2024Quote: ... we first intraperitoneally administrated 50 mg Kg-1 4-hydroxytamoxifen (4-OHT; Sigma, Cat# H6278) into TRAP2/Ai9 mouse line ...
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bioRxiv - Biochemistry 2023Quote: Spin-trapping assays with 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN) (Sigma-Aldrich) were carried out using leaf disks or freshly isolated thylakoid membranes at a concentration of 10 μg of Chl ml-1 ...
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bioRxiv - Cell Biology 2020Quote: ... blocking was performed in 3% BSA in PEM80 for 45 minutes and incubated overnight with a primary antibody against αTub (mouse IgG1, Sigma Aldrich, B-5-1-2, dilution 1:500). The cells were again washed with PBS (3×5 min ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Biophysics 2023Quote: ... cells were resuspended in the appropriate amount of maintaining medium (RPMI Gibco® supplemented with 10% FBS, 2 mM GlutaMAX Sigma®, 1% Penicillin/Streptomycin Sigma®, 1 mM Sodium Pyruvate Gibco) and plated to allow maintenance and amplification ...
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bioRxiv - Bioengineering 2023Quote: ... was added to 1 mL of BG-11 in a 2 mL Eppendorf tube and supplemented with either 1 mM theophylline (Sigma-Aldrich; 100 mM stock dissolved in 100% DMSO) or 1% DMSO as a control and allowed to incubate for 3 days under 50 μmol photons m-2 s-1 prior to conducting the ABTS assays ...
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bioRxiv - Bioengineering 2021Quote: ... A standard solution of poly(3-hydroxy-butyrate) (average Mn ~500,000, Sigma Aldrich) was prepared using the same method ...
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bioRxiv - Molecular Biology 2023Quote: To assess juglone (5-hydroxy-1,4-naphthoquinone, EMD Millipore Cat. No.: 420120-250MG) toxicity ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ml of 2% L-cysteine solution (2 g L-cysteine (Sigma Aldrich, #168149-100G) in 100 ml 0.1% MMR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 150 μL 2× Laemmli SDS-PAGE loading buffer supplemented with 10% 2-mercaptoethanol (Sigma-Aldrich) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG) Assay Kit (Sigma-Aldrich, USA). Paired GBM cell lines were also treated with hit inhibitors individually and in combination ...
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bioRxiv - Neuroscience 2021Quote: ... a selective neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) hydrochloride (Sigma, Dorset, UK) was used 3,4 ...
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bioRxiv - Bioengineering 2021Quote: ... We added 2-amino-thioxanthone (17.2 μmol, 2 equ) in dimethylsulfoxide (DMSO, 0.1 mL, Sigma) to a solution of PA-Rho (8.6 μmol ...
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bioRxiv - Molecular Biology 2020Quote: ... Transduced organoids were selected with 2 rounds of 2 μg/ml of puromycin (Sigma-Aldrich) on day 2 and day 4 ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated with the 0.1mM substrate methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (Sigma) for 1h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then pre-treated with 2mM 2-deoxy-D-glucose (2-DG) (Sigma-D6134) for 15 minutes prior to the assay ...
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bioRxiv - Immunology 2023Quote: ... these cells were pre-treated or not with 2-bromopalmitate (2-BP, Sigma, 21604-1G) at 50 μM for 30 min ...
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bioRxiv - Physiology 2023Quote: ... For sectioned tissues (Figure 2, Supplementary Figure 2) samples were incubated in 30% sucrose (Sigma) overnight (for brains 5 days) ...
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bioRxiv - Physiology 2022Quote: ... 0.1 M N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) sodium salt (Sigma-Aldrich, H7006), 0.12 M sodium chloride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 2.5 μM Oligomycin A (BioShop) or 50 mM 2-Deoxy-D-glucose (2-DG, Sigma) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... #22363548) in a 6 well plate containing 1.35 mM 2’-deoxyguanosine (2-dG, Sigma-Aldrich) in complete DMEM-10 (DMEM ...
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bioRxiv - Synthetic Biology 2022Quote: ... Triplicates of bacterial overnight cultures in Mueller-Hinton broth 2 (MHB 2, Sigma-Aldrich, #90922) were prepared from three different colonies the day before and grown shaking at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... The purified N-glycans were labeled with 2-aminobenzoic acid (2-AA; Sigma-Aldrich, USA) and further purified using a Cyano Base cartridge (Agilent ...
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bioRxiv - Biophysics 2024Quote: ... and buffered with 2 mM N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES, Sigma-Aldrich) at pH 7.4 (called Ham’s F12 observation medium).
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bioRxiv - Developmental Biology 2024Quote: ... Nuclear staining was achieved using 2 mg/mL of 4,6-diamidino-2-phenylindole (DAPI; Sigma). Stained samples were mounted with immunofluorescent mounting medium (DAKO) ...
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bioRxiv - Genomics 2022Quote: ... 4% NP-40) supplemented with 1 mM PMSF and 1:100 dilution of PIC (Sigma P8340) per 10×106 cells ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated in a 1:1 mix of 4% potassium ferrocyanide (Sigma-Aldrich P3289-100G) and 4% HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C (KRASG12D 1:1000, CST #14429; ß-actin 1:2000, SIGMA #A2228 ...
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bioRxiv - Neuroscience 2021Quote: ... A small aliquot from the final suspension was taken and diluted 1:4 in CCM and then 1:1 in Trypan Blue (T8154, Sigma-Aldrich). A live/dead cell count was determined by hemocytometer and a cell viability was assessed ...
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bioRxiv - Cell Biology 2022Quote: For vinculin staining hMSCs are fixed with ice cooled mixture of 1:1 (v/v) (4% PFA: permeabilizing buffer (1% Triton-X-100-Sigma Aldrich) for one minute on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH, 1:10,000 dilution, Millipore; PAFAH/PLA2G7 ...
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bioRxiv - Cancer Biology 2024Quote: ... containing protease inhibitors (1 mM sodium orthovanadate; 1 mM PMSF; 1 mM DTT; 4 mM NaF; protein inhibitor cocktail, P8340 Sigma-Aldrich). Protein concentration was quantified with the DC Protein Assay Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Each IHC staining round was accompanied by nuclear cell counterstaining with 4’,6-diamidino-2-phenylindole (1μg/ml DAPI (Sigma) diluted 1:1000 in PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... cultured neurons from floxed conditional AP-2 KO mice expressing a tamoxifen-inducible Cre recombinase were treated with 0.25 μM (Z)-4-hydroxytamoxifen (Sigma) at DIV3 ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was visualized by FluoProbes 547H (557/572nm) coupled Phalloïdin (Interchim) and nuclei with 0.2 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted in Mowiol (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Cell Biology 2022Quote: ... the nucleus was stained by incubation with 4’,6-diamidino-2-phentylindole (DAPI, Sigma, 200 ng/ml in PBS) for 1 min at RT ...
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bioRxiv - Immunology 2022Quote: ... for 2 or 4 days in complete culture medium composed of RPMI supplemented with 10% foetal calf serum (Sigma), 0.05mM 2-mercaptoethanol ...