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Citations for Millipore Sigma :
3901 - 3950 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Synechococcus WH7803 wild type and mutant cultures were grown in constant light at 12 μmol quanta m−2 s−1 in Pro99 media amended with high molecular weight chitosan or colloidal chitin (Millipore Sigma) to a final concentration of 56 μg/ml in triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... to 20 µl of 60S subunits (blasticidin S dataset) and 1 µl of 2 mM water-solubilised cycloheximide (Sigma-Aldrich 01810) to 20 µl of 60S subunits (cycloheximide dataset) ...
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bioRxiv - Genomics 2022Quote: ... Each lobe was dissected off and minced into small pieces in a beaker containing 39 mL SC-2 supplemented with 1 mL DNase I (Sigma-Aldrich) and 20 mg collagenase P ...
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bioRxiv - Cancer Biology 2023Quote: ... and GSC #161) were obtained from the Mayo Clinic [44] and cultured on laminin-coated plates (1–2 μg/cm2) (Sigma #L2020) in StemPro neural stem cell (NSC ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated in a shaking water bath (180 rpm) at 37°C for 50 minutes with Type 1 Collagenase (2 mg/ml; Sigma-Aldrich) and DNase (100 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The zona free eggs were washed 2× in 1 % BSA in PBS and fixed with 3.7 % paraformaldehyde (P6184, Sigma-Aldrich®) for 20 min and washed 2× in 1% BSA in PBS ...
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bioRxiv - Genomics 2022Quote: ... in PBS) were used for the spectrum codes preparation followed by reacting with 1 μL biotin-NHS (Sigma, 2 mg/mL) for 2 hours in room temperature ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Genomics 2023Quote: ... 0.05% digitonin) containing 2 mM EDTA and a 1:25 dilution of the 0.1 mg/mL BG4 primary antibody (Sigma-Aldrich: MABE917). Primary antibody incubation was performed overnight at 4°C and then the liquid was removed ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Cell Biology 2023Quote: Long-term cultures of MCF10A PLK4/1-608 and MCF7 PLK4 seeded on filter inserts were fixed in 2% glutaraldehyde (Sigma G5882), 2% paraformaldehyde (Fisher Scientific 10131580 ...
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bioRxiv - Microbiology 2022Quote: ... the PVDF-membrane blots were blocked with 5% skimmed milk in PBST for 2 hours at room temperature and then incubated 1 μg/mL anti-Flag mAb (Sigma, F1804), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Systems Biology 2023Quote: ... 50 mL of blocking buffer was prepared per 2 x 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: The coverslip was equilibrated in 1 ml of 30% formamide buffer composed of 2×SSC and 30% deionised formamide (Sigma, F9037) in a 12-well plate for >5 min prior to hybridisation ...
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bioRxiv - Bioengineering 2023Quote: ... three samples of each concentration were incubated in 2 mL of a solution containing 1 mM toluidine blue (no. 89640; Sigma Aldrich) in PBS at pH 10 at room temperature on an orbital shaker set at 150 rpm (no ...
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bioRxiv - Cell Biology 2023Quote: ... the liquid culture phase was collected as a starter culture and diluted 1:100 in fresh BSFB (final volume of 2 L) supplemented with 5 µg/mL vancomycin (Sigma-Aldrich), and incubated on a shaker platform in a humidified ...
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bioRxiv - Developmental Biology 2023Quote: ... were harvested and fixed for 1 h at RT with 2% PFA and Glutaraldehyde in 0.1M phosphate buffer pH7 and 1% sucrose (all from Sigma-Aldrich, USA). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20 mL of the processed media was concentrated to approximately 1 mL using Amicon Ultra-2 spin-filter with 10 kDa molecular wight cut-off (Millipore, UFC201024).
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bioRxiv - Immunology 2023Quote: ... RBCs depleted splenocytes from these mice were ex vivo stimulated with F(ab’)2 anti-Kappa (1 ug/ml, Sigma-Aldrich) and (or ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... the arrested culture was split into three subcultures: one subculture was treated with phleomycin (2-10 μg·mL−1; Sigma-Aldrich, P9564), a second one with β-estradiol (2 μM ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted in 3D homemade printed chambers covered with coverslips and sealed with a melted paraffin–vaseline mixture (∼2:1 ratio) (Cat. #: P3558, Sigma-Aldrich. Vaseline pure ...
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bioRxiv - Neuroscience 2023Quote: ... A high-titre solution of viral vector (AAV9-syn-jGCaMP7f-WPRE) was diluted to a final ratio of 2:1 with a 20% mannitol solution (Sigma-Aldrich) to promote diffusion ...
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were cleared using a 1:2 mixture of Benzyl Alcohol: Benzyl Benzoate (108006, B6630, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2023Quote: ... samples were dehydrated in series of Methanol and cleared using BABB (a mixture of 1 part benzyl alcohol to 2 parts benzyl benzoate; Sigma-Aldrich, benzyl alcohol ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% BoltTM Bis-Tris gels (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
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bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore, Burlington ...
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bioRxiv - Cancer Biology 2023Quote: ... Stable KD of EIF4G2 was generated by infecting HEC-1A or RL95-2 cells with lentiviruses harboring pLKO.1-puro plasmid expressing shRNA targeting GFP (Control) or shRNA targeting EIF4G2 (Sigma TRCN0000147914), followed by selection using puromycin ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were grown in E2 medium (Westerfield, 2000) with the addition of 200 μM 1-phenyl-2-thiourea (PTU, Sigma, P7629) from 1 day post fertilisation (dpf ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in saline solution 2 ml of Evans blue albumin (EBA, 5% bovine albumin labelled with 1% Evans blue; molecular weight: 68 kDa, Sigma Chemicals) for 1 h.[2] Finally ...
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bioRxiv - Neuroscience 2024Quote: ... third-instar larvae were dissected in ice-cold larval Ringer’s solution with a 1:100 dilution of phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726). Tissues were fixed in 4% paraformaldehyde with 1:100 phosphatase inhibitor cocktail 2 in PBS for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were subsequently blocked with 2% BSA-PBS for 1 hour at room temperature and then stained with 5 µU/mL of phalloidin 568 (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... These cell lines were generated by the transduction with lentiviruses expressing the 2 subunits of a PARP-1-specific zinc-finger nuclease (Sigma, CKOZFN1116). Transduced cells were subjected to single cell-cloning ...
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bioRxiv - Immunology 2024Quote: ... the samples were dehydrated with increasing methanol series and subsequently optically cleared in glass-bottom microwell dishes first with 50 % benzyl alcohol (Honeywell) 1:2 benzyl benzoate (BABB; Sigma–Aldrich) in methanol and then with 100 % BABB ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were transferred to BABB solution (1:2 ratio of benzyl alcohol, Sigma-Aldrich, 305197; and benzyl benzoate, Sigma-Aldrich, B6630) for three hours ...
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bioRxiv - Bioengineering 2024Quote: ... Trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2- propenylidene]malononitrile (DCTB) (100 mg mL-1 in ACN) was used as the matrix without further purification (Sigma-Aldrich). NaTFA salt was used as the ionization agent (1 mg mL-1 in MeOH) ...
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bioRxiv - Cell Biology 2024Quote: Implanted SRA were iodinated by immersion overnight in 1.25% potassium triiodide (Chemondis, II0181) diluted 1:2 in 10% formalin (Sigma-Aldrich, HT501128). After washing with distilled water ...
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bioRxiv - Systems Biology 2024Quote: Mouse ESCs were cultured for at least two passages on 0.1% gelatin and fed every 1-2 days with ESC medium consisting of DMEM (EMD Millipore SLM220B) supplemented with ESC-screened FBS (Hyclone SH30071.03E) ...
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase conjugated secondary antibodies (table 2) were added for 1 h at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... bisphenol C-Cl (CAS no. 14868-03-2) and bisphenol S (CAS no. 80-09-1) were bought from Sigma-Aldrich. Compounds were dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Genetics 2024Quote: ... Membranes were washed again twice with PBST and once with PBS followed by 1-2 min incubation in horseradish peroxidase substrate (Millipore, WBKLS0) before visualization using a Chemidoc MP imaging system (Bio-Rad Laboratories).
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...