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Citations for Millipore Sigma :
3001 - 3050 of 3086 citations for N Acetyl Tizanidine d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Systems Biology 2024Quote: ... Abs were then reacted with 10x molar excess of dibenzocyclooctyne-S-S-N-hydroxysuccinimidyl (DBCO-S-S-NH) ester (Sigma Aldrich, 761532-1mg) for 2 hours at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG2000, Laysan Lipids, Arab, AL) were dissolved in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... was electroblotted (25 V and 1 mA for 20 min) under semi-dry conditions onto a positively charged Hybond®-N+ hybridization membrane (Millipore Sigma, GERPN203B) using a Trans-Blot® TurboTM Transfer System (Biorad ...
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bioRxiv - Microbiology 2023Quote: ... 0.165 M 3-(N- morpholino)propanesulfonic acid (Melford, Suffolk, UK) adjusted to pH 7 by 1 M NaOH (Sigma-Aldrich, Burlington, MA, USA)) ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were harvested at E11.5 and N=3 WT and mutant forelimbs (pooled left and right) were dissociated into single cell suspensions using collagenase (Sigma C9697; 50mg/mL) at 37°C for 15 minutes followed by gentle pipette mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Neuroscience 2022Quote: To prepare cell nuclei on the day of library preparation frozen tissue was promptly transferred from -80 °C storage to a chilled 2mL dounce with chilled Nuclei EZ Lysis Buffer (Sigma-Aldrich N 3408). Sample was dounced 25 times with pestle A and 20 times with pestle B ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Biochemistry 2022Quote: ... only C501 and C527 of C2-TRAP1 variant) were blocked by incubating proteins with 10 mM N-ethylmaleimide (NEM, Sigma-Aldrich, Missouri, USA) for 30 min at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... The filtered sample was loaded at 0.5 mL/min onto an AKTA FPLC system fitted with either a Mono S 5/50 GL column or freshly prepared 5 mL fine mesh cellulose phosphate column (Sigma P/N C2258) equilibrated in binding buffer containing 50 mM HEPES (pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Neuroscience 2024Quote: ELANE catalytic activity was determined using the synthetic substrate N-methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (M4765, Sigma-Aldrich, St. Louis, MO). In brief ...
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bioRxiv - Neuroscience 2024Quote: ... mice were intra-amygdalally (i.a.) injected (ventral [V], −2.0 mm below the guide cannula) with either kainic acid (i.a.KA, Sigma-Aldrich, USA; 0.3 µg/2µL; n=30) to induce status epilepticus or sterile phosphate-buffered saline (i.a.PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Rats that underwent training received bilateral infusions of vehicle (n = 4) or one of three doses of 7,8-DHF (Sigma-Aldrich, St. Louis, MO): 1 µg (n = 4) ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Physiology 2022Quote: ... cells were washed two times with PBS+/+ and medium was replaced with glucose-free DMEM (catalog n°D5030, Sigma-Aldrich, Saint-Louis, MO, USA) without phenol red supplemented with 2 mM L-glutamine ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... and kept for 60 s at pH 7.4.The mixture was digested with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 200 μg/mL in the presence of 1% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a sub-set of benzene and air-exposed mice (n=24/group) were treated with 0.5 mg/kg lipopolysaccharides (LPS, Sigma Cat# 2630, Lot# 028M4022V; i.p). At the end of the exposure protocol ...
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bioRxiv - Biophysics 2020Quote: ... The ClyA monomers were purified using Ni+-NTA affinity chromatography and oligomerized by incubation in 0.2 % D-maltoside n-dodecyl-β-D-maltopyranoside (Sigma-Aldrich, Zwijndrecht, The Netherlands) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Neuroscience 2020Quote: ... The cleared hemispheres were then transferred to CUBIC-RA solution (45% w/w antipyrine, 30%w/w N-methylnicotinamide, in H2O, Sigma Aldrich, refractive index=1.5) and again kept at 37°C under continuous shaking ...
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bioRxiv - Biochemistry 2021Quote: ... All standard amino acids were purchased from AAPPTec except for: N-α-Fmoc-O-t-butyl-L-trans-4-hydroxyproline (Hyp) was purchased from EMD Millipore (Billerica, MA), Fmoc-γ-carboxy-glutamic acid γ,γ-di-t-butyl ester (Gla ...
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bioRxiv - Microbiology 2020Quote: ... thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Microbiology 2023Quote: ... #M-3258), TMAO (Trimethylamine N-oxide, #317594), NaClO (Sodium hypochlorite #425044), and BV (Benzyl Viologen, #B8133) were all purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Cancer Biology 2022Quote: ... Two weeks following infection, the drinking water provided to hamsters was supplemented with dimethylnitrosamine (DMN) (synonym, N-nitrosodimethylamine) (Sigma-Aldrich, Inc., St. Louis, MO) at 12.5 ppm until 10 weeks following infection (7 ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Immunology 2024Quote: Biotinylated monomers were diluted to a concentration of 0.5 mg/mL with 0.1 M phosphate buffer and incubated with 0.4 mg/ml of at 37°C for 72 h in the presence of 2.5 mg/ml n-Octyl β-D- glucopyranoside (OG) and 1 mM Pefabloc SC (Sigma–Aldrich, St. Louis, MO). Peptide loaded monomers were subsequently conjugated into tetramers using R-PE streptavidin (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... six-to eight-week-old ΔSPC and wild type FVB/N mice were intraperitoneally (i.p.) injected with urethane (1 mg/g body weight, Sigma-Aldrich, St. Louis, MO, USA) once a week for six consecutive weeks [6] ...
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bioRxiv - Bioengineering 2023Quote: ... poly[di(carboxyphenoxy)phosphazene] disodium salt (PCPP, 1 MDa), spermine tetrahydrochloride, calcium chloride dihydrate (CaCl2), and sodium hydroxide solution (NaOH, 1 N) were purchased from Sigma-Aldrich (St. Louis, MO). Anhydrous sodium sulfide (Na2S ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Cell Biology 2023Quote: RNA was individually extracted from 10 larvae each group (control and hypergravity) for one biological replicate (N = 20 total) with TRIzol (T9424, Sigma-Aldrich, St. Louis, Missouri) according to manufactured procedures ...
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bioRxiv - Neuroscience 2022Quote: ... and cervical spinal cord tissues (C3-C6) were isolated (n=5-8/treatment; 1 male and 1 female per litter) and sonicated in Tri-Reagent (Sigma, St. Louis, MO, USA) and stored at −80°C ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DPPE-PEG5K) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was procured from Fisher Scientific (Pittsburg ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were pipetted onto a pad of 2% agarose prepared in YES media with 0.1 mM N-propyl gallate (Sigma-Aldrich, catalog no. P-3130) and 1% gelatin (Millipore ...
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bioRxiv - Immunology 2024Quote: ... the allosteric FFA2R modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide) and adenosine 5′-thriphosphate disodium salt hydrate (ATP) were from Sigma-Aldrich (Merck, Burlington, MA, USA). The FFA2R antagonist CATPB ((S)-3-(2-(3-Chlorophenyl)acetamido)-4-(4-(trifluoromethyl ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was transferred to a positively charged Amersham Hybond N+ membrane (Cytiva, Cat. No. RPN303B) overnight using capillary action with 20x SSC buffer (Sigma-Aldrich, Cat. No. 33220). Following transfer ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1×106 cells (n=2) were collected per sample and transferred into a Nunc MicroWell 96-well polypropylene plate (Millipore Sigma, cat#P6866-1CS) for staining ...
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bioRxiv - Cancer Biology 2021Quote: ... For AR and FOXA1 ChIP organoids were double fixed (Singh et al., 2019) with 2 mM Di(N-succinimidyl) glutarate (Millipore Signal, Cat #80424-5MG-F) and then 1% formaldehyde ...
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bioRxiv - Physiology 2022Quote: ... and streptomycin (100 μg/ml) and in presence or absence (negative control) of 20 mM sodium lactate (catalog n°L7022, Sigma-Aldrich, Saint-Louis, MO, USA), 1,5 mM sodium pyruvate (catalog n°11360-070 ...