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Citations for Millipore Sigma :
3001 - 3050 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The ClyA monomers were purified using Ni+-NTA affinity chromatography and oligomerized by incubation in 0.2 % D-maltoside n-dodecyl-β-D-maltopyranoside (Sigma-Aldrich, Zwijndrecht, The Netherlands) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Neuroscience 2020Quote: ... The cleared hemispheres were then transferred to CUBIC-RA solution (45% w/w antipyrine, 30%w/w N-methylnicotinamide, in H2O, Sigma Aldrich, refractive index=1.5) and again kept at 37°C under continuous shaking ...
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bioRxiv - Biochemistry 2021Quote: ... All standard amino acids were purchased from AAPPTec except for: N-α-Fmoc-O-t-butyl-L-trans-4-hydroxyproline (Hyp) was purchased from EMD Millipore (Billerica, MA), Fmoc-γ-carboxy-glutamic acid γ,γ-di-t-butyl ester (Gla ...
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bioRxiv - Microbiology 2020Quote: ... thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA ...
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bioRxiv - Neuroscience 2022Quote: ... and cervical spinal cord tissues (C3-C6) were isolated (n=5-8/treatment; 1 male and 1 female per litter) and sonicated in Tri-Reagent (Sigma, St. Louis, MO, USA) and stored at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Two weeks following infection, the drinking water provided to hamsters was supplemented with dimethylnitrosamine (DMN) (synonym, N-nitrosodimethylamine) (Sigma-Aldrich, Inc., St. Louis, MO) at 12.5 ppm until 10 weeks following infection (7 ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Immunology 2024Quote: Biotinylated monomers were diluted to a concentration of 0.5 mg/mL with 0.1 M phosphate buffer and incubated with 0.4 mg/ml of at 37°C for 72 h in the presence of 2.5 mg/ml n-Octyl β-D- glucopyranoside (OG) and 1 mM Pefabloc SC (Sigma–Aldrich, St. Louis, MO). Peptide loaded monomers were subsequently conjugated into tetramers using R-PE streptavidin (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... six-to eight-week-old ΔSPC and wild type FVB/N mice were intraperitoneally (i.p.) injected with urethane (1 mg/g body weight, Sigma-Aldrich, St. Louis, MO, USA) once a week for six consecutive weeks [6] ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Microbiology 2023Quote: ... #M-3258), TMAO (Trimethylamine N-oxide, #317594), NaClO (Sodium hypochlorite #425044), and BV (Benzyl Viologen, #B8133) were all purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Bioengineering 2023Quote: ... poly[di(carboxyphenoxy)phosphazene] disodium salt (PCPP, 1 MDa), spermine tetrahydrochloride, calcium chloride dihydrate (CaCl2), and sodium hydroxide solution (NaOH, 1 N) were purchased from Sigma-Aldrich (St. Louis, MO). Anhydrous sodium sulfide (Na2S ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Cell Biology 2023Quote: RNA was individually extracted from 10 larvae each group (control and hypergravity) for one biological replicate (N = 20 total) with TRIzol (T9424, Sigma-Aldrich, St. Louis, Missouri) according to manufactured procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DPPE-PEG5K) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was procured from Fisher Scientific (Pittsburg ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were pipetted onto a pad of 2% agarose prepared in YES media with 0.1 mM N-propyl gallate (Sigma-Aldrich, catalog no. P-3130) and 1% gelatin (Millipore ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Cancer Biology 2021Quote: ... For AR and FOXA1 ChIP organoids were double fixed (Singh et al., 2019) with 2 mM Di(N-succinimidyl) glutarate (Millipore Signal, Cat #80424-5MG-F) and then 1% formaldehyde ...
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bioRxiv - Physiology 2022Quote: ... and streptomycin (100 μg/ml) and in presence or absence (negative control) of 20 mM sodium lactate (catalog n°L7022, Sigma-Aldrich, Saint-Louis, MO, USA), 1,5 mM sodium pyruvate (catalog n°11360-070 ...
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bioRxiv - Neuroscience 2020Quote: ... SND75 devices and Aclar sheets were coated separately using 100 μg/ml poly L-ornithine (PLO) (Sigma, St. Louis, MO USA, Cat N° P3655-100MG) in DPBS (Cat N° 14190250 ...
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bioRxiv - Microbiology 2021Quote: ... the MDCK monolayers were washed twice to remove FCS using PBS containing 1 μg/mL of trypsin treated with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) (Sigma-Aldrich, St. Louis, Mo, USA), inoculated with each sample ...
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bioRxiv - Physiology 2022Quote: ... and Trpc6E897K/E897K (n=15) mice were given daily injections of low-endotoxin bovine serum albumin (BSA, A-9430, Sigma Chemical Co, St. Louis, MO) in sterile saline (300mg/ml ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... ground and homogenized western corn rootworm larval tissue (3-7 larvae per biological replicate, n=8-11) using the GenElute Universal Total RNA Purification Kit (Sigma-Aldrich, St. Louis, MO, USA). A NanoDrop spectrophotometer (ND-1000 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7) gradient (20%, 40%, 60%, 80%, 100%), incubated 3 times (1h, o/n, 2h) in 100% dichloromethane (DCM) (Sigma-Aldrich, St. Louis, MO, USA) to remove hydrophobic lipids ...
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bioRxiv - Microbiology 2020Quote: ... The optimization of the analytical parameters in MS was performed by infusing the internal standard N-heptanoyl-L-homoserine lactone (C7-HSL) (Sigma-Aldrich, Saint Louis, MO, USA).
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted from tail tissues and amplified with specific PCR primers using REDExtract-N-Amp™ Tissue PCR kit (Cat. No. XNAT-100RXN, Sigma-Aldrich, Saint Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue digestion for DNA extraction and subsequent polymerase chain reaction (PCR) was performed with the prepared sample buffer from REDextract-N-Amp PCR KIT (Sigma-Aldrich, St. Louis, United States) or DreamTaq™ Hot Start Green DNA Polymerase (Thermo Fischer Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... crosslinking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Carl Roth GmbH + Co. KG) and N-hydroxysuccinimide (NHS) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was applied ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Neuroscience 2020Quote: ... fixed with vacuum grease and kept in physiological Tyrode’s solution for imaging (in mM: 120 NaCl, 2.5 KCl, 30 glucose, 2 CaCl2, 1 MgCl2, and 20 HEPES; Sigma Aldrich; pH = 7.2-7.4; n = 1.33).
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bioRxiv - Neuroscience 2021Quote: ... Oocytes were mechanically homogenized and membrane proteins were extracted using DDM (100 mM phosphate buffer, 150 mM NaCl 0.5 % n-dodecyl-β-D-maltoside, SIGMAFAST Protease Inhibitor Cocktail (Sigma-Aldrich, St. Louis, MO, USA); pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Cancer Biology 2022Quote: Western blotting of nuclear extracts or whole cell extracts was performed by standard methods using antibodies specific for the N-terminal portion of ETV6 (Sigma, # HPA000264; Santa Cruz, #sc-166835x), CTCF (Cell Signaling ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... in case of dissolving and removing the remaining oil, 200 μL n-Hexane (Aladdin, catlog no. H140380-1L) with 1% (v/v) span80 (Sigma-Aldrich, catalog no. S6760-250ML) was added to microgels to dissolve any remaining oil ...
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bioRxiv - Microbiology 2022Quote: ... each flask was spiked with 3 ml of 100 mg NH4+-N L-1 NH4Cl or 100 mg Mn2+ L-1 MnCl2·4H2O (Sigma Aldrich, Saint Louis, Missouri USA). Liquid samples (1 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Single colony was picked up into 50 ml 3-(N-morpholino) propane sulfonic acid medium (MOPS) buffered defined liquid medium (Sigma-Aldrich, St. Louis, MI, USA). For DSM1092 ...
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bioRxiv - Cell Biology 2021Quote: ... 40,000 cells/well for COS-7 cells and 30,000 cells/well for MEFs (Wild-type or Drp1 KO) were seeded into poly-D-lysine-coated 8 well glass chamber (Sigma, P6407-5MG, Cellvis, C8-1-N). The next day ...
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bioRxiv - Immunology 2021Quote: ... were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no. T7408-500ML + 0.05% n-Dodecyl β-D-maltoside, Millipore Sigma, catalog no. D4641-1G). Protein was quantified using Pierce BCA kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Mouse groups (n=4) were injected with 100μl of vehicle (PBS, or No Drug) or immunosuppressants: cyclophosphamide monohydrate (CYC, Sigma Aldrich C0768, 60mg/kg every other day), leflunomide (LEF ...
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bioRxiv - Cell Biology 2024Quote: Lyophilized N-glycan samples were resuspended in 200 µL of a freshly prepared NaOH/DMSO slurry (7 NaOH pellets, Sigma-Aldrich #S8045; grounded in ∼3mL DMSO) and 100 µL of iodomethane (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Neuroscience 2024Quote: Reagents for the nitrite assay (sodium nitrite standard [CAS # 7632-00-0]: Sigma-Aldrich 237213, ACS reagent, ≥97.0%; sulfanilamide [CAS #63-74-1]: Sigma-Aldrich S9251, ≥98.0%; N-(1-naphthyl)ethylenediamine [CAS #1465-25-4] ...
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bioRxiv - Microbiology 2024Quote: ... Neuraminidase inhibition test was measured using 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (Munana; Biosynth AG, Staad SG, Switzerland and Sigma-Aldrich, St. Louis, Missouri, USA) as substrate ...
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bioRxiv - Microbiology 2024Quote: Sulfolobus acidocaldarius MW001 was grown in Brock medium (Brock et al., 1972) supplemented with 0.1% (w/v) N-Z amine (Sigma-Aldrich®, Merk KGaA, Burlington, MA, USA), 0.2% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated overnight at 4 ° C using antibodies specific for the N-terminal amino acids 66 – 81 of the amyloid precursor protein (APP) (Millipore, Burlington MA, clone 22C11 at 1:80K), GFAP (Leica ...