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Citations for Millipore Sigma :
2701 - 2750 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Immunology 2023Quote: ... were plated one day prior to the coculture with γδ T cells at a density of 15.000 cells/well in a flat-bottom 96-well plate and treated O/N with 10 μM pamidronate (PAM, Sigma) in complete IMDM or DMEM ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Neuroscience 2023Quote: ... or acidic pH 6.5 media (26mM NaHCO3, 20mM 2-(N-morpholino) ethanesulfonic acid (MES)(Millipore Sigma, St. Louis, MO, USA), 5mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Human neuroblastoma cell line SK-N-SH (ATCC HTB-11) was cultured in Dulbecco’s Modified Eagle’s Medium with 1g/L glucose (Sigma-Aldrich, FIN) supplied with 10 % FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pelleted after 6 h or 12 h and resuspended in SD-N containing 30 mg/l L-lysine-13C6,15N2 hydrochloride (Lys8, Sigma, 608041) and incubated for indicated time ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... N’-tetramethylethane-1,2-diamine (TEMED) and 1% (v/v) of solution of 10% (w/v) ammonium persulfate (APS) (Sigma-Aldrich) was added to the mixture and vortexed to initiate the polymerisation ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Cell Biology 2024Quote: ... Two µl of colorless hemolymph was aspirated from each pile and separately transferred to 96-well plates (Thermo-Scientific) containing 0.1% N-Phenylthiourea (Sigma-Aldrich) in 50Lµl PBS ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was neutralized with 1 N acetic acid until gas evolution ceased and filtered through Celite 545 (EMD Millipore). The solution was concentrated by rotary evaporation at 50 °C and precipitated in cold diethyl ether (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Infant (n=1) and adult (n=1) hippocampal sections (10μm) were fixed in 10% neutral buffered formalin (catalog no. HT501128, Sigma-Aldrich) for 30 minutes at RT ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Biochemistry 2024Quote: N-Lactoyl Phenylalanine (catalogue # 318943) was purchased from Novo Pro Labs and 1-Carboxyethylphenylalanine (ENAH95E73EBB) was purchased from Sigma Aldrich.
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... were identified and quantified by gas liquid chromatography (Perichrom) as their alditol acetates obtained after hydrolysis (4 N trifluoroacetic acid (TFA, Sigma), 100 °C ...
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bioRxiv - Immunology 2024Quote: HAP1 target cell lines were plated one day prior to the coculture with γδ T cells at a density of 15,000 cells/well in a flat-bottom 96-well plate and treated O/N or not with 10 µM pamidronate (PAM, Sigma) in complete IMDM (includes 10% FCS ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... In the reciprocal experiment 1 mg of N-terminally his-tagged Saci_1104 was loaded onto a Nickel-NTA (Sigma, Germany) column (column volume of 250 µl) ...
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bioRxiv - Cell Biology 2024Quote: Tubulin was purified from porcine brain using established protocols as described previously [39] Polarity-marked microtubules were prepared by preferably extending the plus-ends of long GMPCPP seeds in the presence of N-ethylmaleimide (Sigma) modified tubulin (NEM-tubulin) ...
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bioRxiv - Biochemistry 2024Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Plant Biology 2020Quote: ... 150 uM stock trans-zeatin in .01 N KOH was added to autoclaved media to create 150 nM working concentration (Sigma, Z0876). For the NPA experiment ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C in a wet chamber with the following antibodies: anti-P2X7 (cat. n. P8232, Sigma-Aldrich) and anti-Tom 20 (cat n ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Steidle and Dettner, 1993) in 70 μl hexane (company, GC/MS analytical grade) containing 150 ng/μl n-octadecane (Sigma Aldrich) as an internal standard ...
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bioRxiv - Molecular Biology 2020Quote: ... and mice exposed to 5 days of photo-oxidative damage (PD) (n=6) were lysed in CellLyticTM Cell Lysis Buffer (Sigma-Aldrich) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 5% lysed horse blood (defibrinated 50% stock, Hatunalab AB Cat. N° 139) and 20 mg/mL β-NAD (Sigma, Lot# SLCD5502). Prior to use the 50% horse blood stock was freeze thawed five times and clarified via centrifugation twice for 30 minutes at 18,000 rpm at 4 °C and then filtrated using 0.2 μm membrane filter ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we treated females in the Pregnancy GCs (n = 44 total litters from 43 unique females) and Lactation GCs (n = 18 litters from 17 unique females) treatment groups with exogenous cortisol (hydrocortisone, Sigma H4001) dissolved in peanut butter and wheat germ mixture (8 g of peanut butter ...
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bioRxiv - Microbiology 2019Quote: ... cells were transferred (2% inoculation) into 50 ml of T-R minimal medium supplemented with 0.5% (w/v) N-acetylglucosamine (Sigma-Aldrich #G4875) as a carbon source and incubated ~12 hr at room temperature and 150 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Retinal explants were grown as for DRGs but without NGF and with medium supplemented with N-2 (Sigma-Aldrich, 100x concentrate) and bovine pituitary extract (200μg/ml ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Peaks were tentatively identified by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... whole brains of females (n = 6) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Bioengineering 2021Quote: ... The MCF-7 and the SK-N-SH cells were cultured in RPMI 1640 and in Minimum Essential Medium Eagle (MEM) from Sigma Aldrich, respectively ...
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bioRxiv - Biochemistry 2020Quote: We fused FtsE at its N-terminus to a 6xhis-followed by a TEV protease cleavage site in vector pETDuet™-1 (Novagen) that allows tunable expression of the fusion protein from a T7 promoter induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on two connected in series 1 ml Heparin-Sepharose columns (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on a Superdex 200 10/300 GL size exclusion column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... myotube compartments were treated with 19 μM of the AChR competitive antagonist tubocurarine hydrochloride pentahydrate (DTC) (Sigma, Cat N° T2379-100G) 10 min before analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Biophysics 2020Quote: ... The coating was done in the following three step procedure: I) 1mL of the resulting solution was added to 11.5 mg NHS (N-Hydroxysuccinimide, Sigma-Aldrich, Germany) and vortexed ...