Labshake search
Citations for Millipore Sigma :
2551 - 2600 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
-
bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
-
bioRxiv - Cancer Biology 2020Quote: ... Coding regions of wildtype BAG2 and ZNF451-BAG2 were amplified from SK-N-AS cells for subcloning into p3XFLAG-CMV14 (Sigma) and pLVX-EF1α-IRES-mCherry (Clontech ...
-
bioRxiv - Immunology 2022Quote: ... the DNA sequence for the synthetic N gene (Met1 to Ala 419) was amplified and cloned into the NdeI and XhoI digested pET24a(+) vector (Novagen).
-
bioRxiv - Developmental Biology 2019Quote: ... Villin-CreERT2 duodenum (n=4 per genotype) were treated two days after seeding with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) 1.25 µM for 15h ...
-
bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
-
bioRxiv - Biochemistry 2019Quote: ... Xa factor site and StreptagII sequence in its N-terminus are digested by HindIII and BamHI and then inserted into a pET-20b plasmid (Novagen). The over-expression in BL21 (DE3 ...
-
bioRxiv - Biochemistry 2019Quote: ... Xa cleavage site and StrepTagII sequence in its N-terminus part were digested with XbaI and Hind III enzymes and inserted into pET-20b plasmid (Novagen). The over-expression in Rosetta (DE3 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Hybridization was performed at 50°C O/N with the probe 1.6 kb fragment containing the sequence (TTAGGG)n63 labelled with Nick Translation Kit (Sigma-Aldrich) and developed according to62 ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used include anti-α and β-Synuclein (EP1646Y, N-terminal rabbit monoclonal, 1:100 dilution; Millipore, Billerica, MA), anti-pS129 αSyn (81A ...
-
bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
-
bioRxiv - Cell Biology 2020Quote: ... the 1.01 µm diameter carboxylated silica microspheres (SC04000, Bangs Beads) were activated by amine-reactive crosslinker chemistry using NHS (N-hydroxysuccinimide, 130672-5G, Sigma) and functionalized by gelsolin (HPG6-A ...
-
bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and 50mM Tris pH 8.0) supplemented with 10 mM freshly prepared N-ethylmaleimide and protease inhibitors (Sigma-Aldrich). Washed GFP-Trap A beads (Chromotek ...
-
bioRxiv - Cancer Biology 2019Quote: PyMT [FVB/N-Tg(MMTV-PyVT)634Mul/J] transgenic animals harboring breast tumors were treated with 4-hydroxytamoxifen (Sigma-Aldrich) (1.2 mg/kg/daily ...
-
bioRxiv - Biochemistry 2020Quote: ... Fl-SNED1 and the N-terminal fragment were purified by affinity chromatography on an anti-FLAG resin (Sigma-Aldrich, A2220) as previously described (58 ...
-
bioRxiv - Plant Biology 2021Quote: ... The released N-glycans were purified on graphitized carbon SPE columns (Supelclean™ ENVI-Carb™, Sigma-Aldrich, Poznań, Poland) and labeled with a fluorescent tag (anthranilic acid ...
-
bioRxiv - Plant Biology 2020Quote: ... the pellet was resuspended in minimal volume of 50 mM MOPS (pH 7.5) before being digested with Asp-N endoproteinase (Sigma-Aldrich) at 1:50 (enzyme:acyl-ACP ...
-
bioRxiv - Microbiology 2021Quote: ... cells were cultured in MDCK medium and after inoculation in infection medium containing N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (3µg/mL; Sigma, T1426).
-
bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
-
bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
-
bioRxiv - Neuroscience 2019Quote: ... injection of saline (10 ml/kg body weight) or clozapine N-oxide (CNO, 10 mg/kg, 10 ml/kg, Sigma). Drugs (saline or CNO ...
-
bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
-
bioRxiv - Neuroscience 2021Quote: All primers were provided from Thermo Fisher and the REDExtract-N-Amp PCR Reaction Mix™ reagent used for the polymerase reaction was provided from Sigma-Aldrich® ...
-
bioRxiv - Biochemistry 2020Quote: ... the reactions were stopped with EDTA buffer and the samples incubated with 0.1U/mL of chondroitinase ABC (AMS Biotechnology, Abingdon, UK) and keratanase (endo-beta galactosidase, Cat. n.: G6920, Sigma Aldrich) in deglycosylation buffer (50mM sodium acetate ...
-
bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
-
bioRxiv - Biochemistry 2020Quote: ... The reaction was initiated by the addition of 1 mM N-α-benzoyl-L-arginine-p-nitroanilide substrate (Sigma-Aldrich) and incubated for 30 min at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
-
bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
-
bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
-
bioRxiv - Cell Biology 2021Quote: Liver nuclei (n=4) were prepared from frozen tissue specimens by crushing and dounce homogenising the tissue in 1 ml EZbuffer (SIGMA) (20 strokes with loose and a tight pestle ...
-
bioRxiv - Biochemistry 2021Quote: ... Cysteine Biotinylation was carried out using Poly(ethylene glycol) [N-(2-maleimidoethyl)carbamoyl]methyl ether 2-(biotinylamino)ethane (Sigma 757748) (Biotin-maleamide) ...
-
bioRxiv - Biochemistry 2020Quote: ... Specifically the dried extracts were dissolved in 0.2-mL acetonitrile and silylation was performed by the addition of 0.05-mL of N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) (Sigma-Aldrich), and incubated at 65°C for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
-
bioRxiv - Plant Biology 2020Quote: ... was cloned using cDNA from Arabidopsis seedlings and inserted in frame with an N-terminal His6-tag between the BamHI and EcoRI sites of the pETDuet-1 vector (Novagen). Protein expression was performed in E ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
-
bioRxiv - Cell Biology 2020Quote: ... was added at equal volume and supplemented with 10 μL anti-fade solution (9 parts glycerol, 1 part 10X PBS, 0.1 part 20%(w/v) n-propyl gallate (Sigma P3130) in dimethyl sulfoxide) ...
-
bioRxiv - Neuroscience 2019Quote: ... rats were injected (1 mL/kg; i.p.) with either the cannabinoid agonist CP-55,940 (n=36; C112-10MG; Sigma-Aldrich, Inc.) or a control vehicle (n=11 ...
-
bioRxiv - Neuroscience 2019Quote: ... Supernatants were immediately used for measurement of proteasome peptidase activity by cleavage of the fluorogenic peptide N-Suc-LLVY-MCA (Sigma), enzymatic activity were acquired for 2h ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µg of purified MurA protein were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction buffer containing 100 mM Tris-HCl pH8.0 and 150 mM NaCl (final volume ...
-
bioRxiv - Plant Biology 2021Quote: ... Free hydroxyl FAs were derivatized at 110°C for 30 min in 100 µL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA, Sigma) and pyridine (50:50 ...
-
bioRxiv - Neuroscience 2021Quote: ... four brains were pooled (n=6) and homogenized in 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
-
bioRxiv - Cell Biology 2021Quote: ... cycloheximide (C4859) and the tankyrase inhibitor IWR-1 (I0161) and the deubiquitinating enzyme inhibitor N-Ethylmaleimide (NEM) (E3876) were from Sigma. The tankyrase inhibitor Az6102 (S7767 ...
-
bioRxiv - Cell Biology 2020Quote: ... mADD1 has 6xHis tag at its N terminus followed by TEV protease recognition site and the plasmid was transformed into Rosetta (DE3) pLysS cells (Novagen). Target protein was expressed in cultures grown in autoinduction media at 18°C overnight 48 ...
-
bioRxiv - Cell Biology 2021Quote: ... 50 mg/mL EDC and NHS solutions were prepared in 25 mM cold 2-(N-morpholino) ethanesulfonic acid (MES, cat. num. M3671-50G, Sigma), at pH5 ...
-
bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
-
bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
-
bioRxiv - Pathology 2022Quote: ... The resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) [both purchased from Sigma] ...
-
bioRxiv - Microbiology 2022Quote: ... BHI was supplemented with BC (n-alkyl dimethyl benzyl ammonium chloride, Sigma-Aldrich, St. Louis, MO, CAS: 63449-41-2) to achieve appropriate BC target concentrations ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
-
bioRxiv - Developmental Biology 2022Quote: ... a small population (n≈10) of 3rd instar larvae were embedded in a previously heated 5% m/v carboxymethylcellulose (Sigma) solution ...