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Citations for Millipore Sigma :
2851 - 2900 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Physiology 2024Quote: ... groups of mixed staged adult Hypsibius exemplaris (n≈20 animals) were collected from culture dishes (at 20°C) via manual microaspiration (a Sigma-Aldrich A517 aspirator tube assembly with a hand-pulled 150mm Glass Pasteur Pipette tip ...
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bioRxiv - Neuroscience 2023Quote: ... mice received systemic injections of the DREADD agonist clozapine N-oxide (CNO, Sigma-Aldrich; 1-1.5 mg/kg dissolved in saline). The control group was injected with saline ...
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bioRxiv - Biochemistry 2023Quote: The remaining 10% of the free N-glycans (see above) was subject to solid-phase extraction on non-porous graphitised carbon (SupelClean ENVICarb, Sigma-Aldrich) and eluted with 40% acetonitrile ...
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
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bioRxiv - Immunology 2023Quote: ... a targeted amplicon library was generated by PCR from the standard gene expression library cDNA using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004). 50uL PCR reactions were prepared by combining 25uL ReadyMix with 2.5 uL of a 10uM stock of each primer (10x Partial Read 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Cell Biology 2023Quote: Tubulin was purified from porcine brain as described before (Castoldi and Popov, 2003) and labeled with biotin-N-hydroxysuccinimide (NHS; Sigma-Aldrich) or ATTO-647N-NHS (ATTO-TEC ...
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bioRxiv - Microbiology 2023Quote: ... tubes were allowed to cool for 10 mins at room temperature and 2 mL of spectroscopy grade n-heptane (Sigma 1043660500) and 667 uL of sterile deionized water were added to each tube and vortexed for 3 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... individual lung lobes were isolated and homogenized in 1 mL PBS and subsequently hydrolyzed through the addition of 1 mL of 12 N HCl (Sigma Aldrich) followed by incubation at 120°C for 18 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Neuroscience 2024Quote: ... These double transgenic animals were separated into 4 groups (n = 3) and injected with a single dose of 4OH-Tx (Sigma-Aldrich). Tissue was collected at 10- ...
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bioRxiv - Neuroscience 2024Quote: ... 14 days later, Clozapine N-oxide (CNO, Cat# HY-17366, MCE, USA) was dissolved in dimethyl sulfoxide (DMSO, Cat# D8418, Sigma, Germany) to create a 100 mg/mL stock solution ...
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bioRxiv - Biochemistry 2024Quote: ... and N-(1-Naphthyl)ethylene diamine dihydrochloride (NEDC) (cat#222488-5G) and penicillin/streptomycin (PEN/Strep; cat# P0781) were from Sigma-Aldrich Chemie GmbH (Taufkirchen ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of N-Flag WT or mutant TTC5 in rescue cell lines was detected by anti-Flag M2-HRP (Sigma A8592), monoclonal anti-Flag M2 (Sigma F3165) ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 15,000 cells per well in 50 µL of medium containing the HDAC substrate N-(4-Methyl-7-aminocoumarinyl)-Nα-(t-butoxycarbonyl)-Nω-acetyllysineamide (21 μM, MAL, Sigma-Aldrich) and incubated for 8 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: The interaction between PRDX6 and SEPHS2 in SK-N-DZ WT and SK-N-DZ PRDX6 KO cells was observed using Duolink in situ proximity ligation assay (PLA) kit (DUO92101, Sigma- Aldrich) in the presence or absence of 600nm Selenocysteine ...
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bioRxiv - Cell Biology 2020Quote: ... 4-well LabTek dishes were incubated 30 min at 37°C with 10 μg/ml fibronectin or O/N at 37°C with 20 μg/ml laminin (Sigma, L2020-1MG). RPE1 cells were then plated at low density in the dishes and imaged the next day ...
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bioRxiv - Cell Biology 2020Quote: ... at pressure range 25 – 30 kPsi with subsequent addition of 0.5 % w/v Triton X-100 (Cavin1 purification) or n-dodecyl β-D-maltoside (DDM) (1.2 mM) (MBP-GBP-CAV1 purification) and 5 mM imidazole (Sigma Aldrich, 792527) followed by centrifugation 35,000x g for 30 min ...
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bioRxiv - Genetics 2021Quote: ... CHCs were extracted by submersing individual wasps for 10 min in 10 µl n-hexane (≥ 99.0%; Sigma-Aldrich, St. Louis, MO, USA) using 1-ml glass vials equipped with a 0.1-ml micro insert (Alltech ...
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bioRxiv - Developmental Biology 2021Quote: ... vehicle control and treated (n=3 per group) were incubated in the sepeate fish tank containing dissolved BrdU (25 μg/L) (Sigma-Aldrich, USA) for 24 hours before tissue collection ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Genomics 2019Quote: ... engrafted colonic tissues were finely minced with a razor then transferred to a Dounce tissue homogenizer (Kimble Chase KT885300-0002) in 2 mL of ice-cold Nuclei EZ Lysis buffer (Sigma #N-3408) supplemented with protease inhibitor (Roche #5892791001 ...
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Physiology 2019Quote: Islets (n≥4 mice/sex/treatment group) were lysed in radioimmunoprecipitation assay buffer with protease inhibitor cocktail (Sigma, St. Louis, MO, USA). Lysates were subjected to SDS-PAGE and blotted using antibodies as detailed in Supplemental Table 3 ...
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bioRxiv - Physiology 2021Quote: ... Each mouse kidney was minced and homogenized using Dounce homogenizer (5x loose, 10x tight, Kimble Chase KT885300-0002) in 2 mL of Nuclei EZ lysis buffer (Sigma N-3408) supplemented with SuperaseIn and cOmplete ULTRA Tablets Mini (Roche ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Bioengineering 2020Quote: ... 9µL antibody solutions in phosphate buffered saline (PBS) at 1-2 mg/ml were combined with 1 µL 2mM dibenzocyclooctyne-PEG4-N-hydroxysuccinimidyl ester (Sigma, Catalog # 764019) in dimethylsulfoxide (DMSO ...
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bioRxiv - Immunology 2021Quote: ... subsequently lysed and postnuclear supernatants were incubated o/n with monoclonal mouse anti-HA-antibody coupled to agarose beads (clone HA-7, Sigma-Aldich, USA) at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... An emulsion was generated by injecting 50 μL of pre-bead-solution in 500 μL of n-hexane with 3% Span®80 (Sigma-Aldrich) using a 100 μL Hamilton Syringe ...
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bioRxiv - Bioengineering 2020Quote: ... collagen type I was extracted from rat tail tendons as freeze-dried sponges according previously published protocols.42,43 The extracted collagen sponges were dissolved in 0.02 N acetic acid (cat. no. 695092, Sigma-Aldrich, St. Louis, MO) to prepare a sterile stock collagen solution ...
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bioRxiv - Biochemistry 2021Quote: ... from the free radioactivity by transferring the cell suspension to a 0.4 mL centrifuge tube (USA Scientific, Ocala, FL) with a layer of 1:37.5 n-dodecane:bromo-dodecane (Sigma–Aldrich, St. Louis, MO) and spinning at 12,535 x g ...
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bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... 5×106 cells/ ml) in N-free medium were seeded on cover slips coated with poly-L-lysine (Sigma, Cat. No. P8920) for 5 minutes followed by fixation with 4% paraformaldehyde solution (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 34.5 mL of the liquid fertilizer was mixed with 0.85 g 15N labelled N (98 atom% 15N, in the form of 15NH4 15NO3, Sigma-Aldrich, Buchs, Switzerland). The amount of 15N corresponded to 8% of the total N given ...
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bioRxiv - Microbiology 2021Quote: ... and trimethylsilylated shortly before analysis through addition of 20 μl N,O-bis(trimethylsilyl)trifluoroacetamide with 1 % trimethylchlorosilane (BSTFA-TMCS, Cerilliant, Sigma B-023). The GC-MS instrumentation and settings (electron ionization mode ...
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bioRxiv - Cell Biology 2020Quote: ... IMR90 cells (n=2 biological replicates) stably expressing ZNF416-FLAG were cross-linked with 1% formaldehyde (Sigma Aldrich, St. Louis, MO, USA) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl, 2 mM MgSO4, 0.2% n-dodecyl-β-D-maltoside [Nacalai Tesque, 14239-54] with protease inhibitor [Sigma-Aldrich, P8340]). After 20 min of incubation on ice ...
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bioRxiv - Bioengineering 2019Quote: Samples were taken from scaffolds using a 2mm biopsy punch (n=5) and incubated with 1% alizarin red s (Sigma Aldrich, A5533) in distilled water for 10 min at a pH of between 4.1 - 4.3 ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Swiss-Webster mice (n=5/group) were injected with 75 µg of ferrous chloride (FeCl2·4H2O, Sigma-Aldrich Inc., St. Louis, MO) by the intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...