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Citations for Millipore Sigma :
2651 - 2700 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Immunology 2021Quote: Transformed bacteria were grown overnight (o/n) in 5 mL of 2x YT (tryptone + yeast extract + NaCl BioXtra, Sigma Aldrich) medium supplemented with Amp100 and 1% of glucose (Glc1% ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were incubated O/N at 4°C followed by two washing steps of 5 minutes with Wash Buffer A (Sigma) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (f-MLP) (F3506) and prostaglandin E1 (PGE1) were all purchased from Sigma Aldrich. The purinergic receptor agonist ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Plant Biology 2023Quote: ... flowers were treated for three consecutive days one week after bolting with the solution containing 30 μM 1-N-naphthylphthalamic acid (Sigma) and 0.010 % (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... At this point the hct was lowered to 2.5% and the culture was treated for 48–72 h with 50 mM N-acetylglucosamine (NAG; Sigma-Aldrich) in order to clear residual asexual parasites and obtain a virtually pure gametocyte culture ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM Na-pyro-phosphate in PBS) supplemented with 10 mM N-ethylmaleimide and Complete EDTA-free protease inhibitor cocktails (Sigma). Lysates were centrifuged at 14,000 rpm for 20 min at 4°C and cleared with protein G-sepharose beads ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids used to produce ActRII and ActRIIB in bacteria were constructed by inserting the corresponding coding sequence downstream of the N-terminal His6 tag and thrombin cleavage site in pET15b (EMD-Millipore). The plasmid used to produce BMPRII extracellular domain in mammalian cells was constructed by inserting the coding sequence for this downstream of the rat serum albumin signal peptide ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Neuroscience 2024Quote: RNA extraction was done from age-matched adult Drosophila heads (n=60) using Trizol (Cat no. T9424, Sigma-Aldrich, USA) reagent as per manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Gedunin (Gaia Chemicals, USA) and the specific HSP90 inhibitor 17-AAG (17-N- allylamino-17-demethoxygeldanamycin, Tanespimycin, Sigma-Aldrich, USA) were dissolved in DMSO (dimethyl sulfoxide ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Neuroscience 2023Quote: ... the bath application of 30 μM 5-HT together with 50 μM N-methyl-DL-aspartic acid (NMA, Sigma-Aldrich) was applied to the isolated brains ...
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bioRxiv - Microbiology 2022Quote: ... NSm and the entire pcDNA3.1 vector encoding Gn with an N-terminal SS plus HA tag were amplified by PCR using KOD Hot Start DNA polymerase (Novagen), mixed and then concatenated using the NEBuilder HiFi assembly kit according to the manufacturer’s instructions to yield pSnH-OROV-M ...
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bioRxiv - Molecular Biology 2023Quote: ... or full length DDX50) and 50 ng N-terminally tagged HaloTag-GID4 using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The obtained plasma from control and depleted fish (n = 18-32 fish) was diluted 10-folds with endotoxin-free PBS (Millipore) and heated at 70 °C for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Plant Biology 2022Quote: ... The dried wax residues were derivatized using 200 μL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) (derivatization grade, Sigma-Aldrich)/pyridine mixture (1:1 ...
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bioRxiv - Developmental Biology 2023Quote: ... single CC-hPSCs were inoculated at a density of 8×104 cells per 3.5 cm dish onto feeders in AIC-N medium supplemented with 1mM Valproic acid (VPA, Sigma, P4543) 36 ...
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bioRxiv - Biochemistry 2023Quote: ... male and female Il10−/− mice (n = 7 per sex) were switched to a diet containing 100 ppm of piroxicam (Sigma) (Envigo ...
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bioRxiv - Neuroscience 2023Quote: ... or vehicle comprised of 10% dimethyl sulfoxide (DMSO) in saline (n = 20) all acquired from Sigma Aldrich (St. Louis, Missouri). On the eighth day ...
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bioRxiv - Microbiology 2023Quote: ... 22 mm x 22 mm #1.5 borosilicate coverslips (DOT Scientific) were placed in a Wash-N-Dry™ coverslip rack (Sigma) and submerged in ∼80 mL of 1 M NaOH in a 100 mL glass beaker ...
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bioRxiv - Molecular Biology 2023Quote: Cys ox-PTMs were labeled using a bioswitch workflow (Fig. 1A and S1A) involving a thiol alkylation step using 2.3mM N-ethylmaleimide (NEM, Sigma Aldrich), followed by reduction of reversibly oxidized thiols using 4mM tris(2-carboxyethyl ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 mL nitrate stock solution (10 g N L-1) containing ∼1% 15N-labelled sodium nitrate (98 atom % 15N, Sigma) was added to achieve a concentration of ∼20 mg N L-1 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an introduction of 0.12 mL nitrate stock solution (40 g N L-1) which contained ∼1% 15N-labelled NO3- (98 atom % 15N, Sigma). The butane culture was flushed with argon gas (99.99% ...
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bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Biochemistry 2023Quote: ... was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were dehydrated with methanol/PBS and incubated O/N at RT in 66% dichloromethane (DCM; cat. #: 270947; Sigma-Aldrich) and 33% methanol ...
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bioRxiv - Developmental Biology 2023Quote: The testes from 8-week-old C57BL/6J wild-type mice or 4-month-old Foxc2f/+ and Foxc2f/-;Ddx4Cre/+ mice (n=4) were minced and digested in the collagenase type IV (1 mg/mL, Sigma) and DNase I (500μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was freshly prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ∼20 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... N-termini were labeled with light and heavy formaldehyde (12CH2O in H2O, and13CD2O in D2O (252549 and 596388, Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2023Quote: Right tibias from SMAD1/5WT and SMAD1/5iΔEC mice (n= 10 per group) were collected and fixed in 4% paraformaldehyde (PFA; Sigma Aldrich) in PBS overnight (12h ...
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bioRxiv - Developmental Biology 2022Quote: ... the dish was filled with embryo water supplemented with MS222 (final concentration 160ng/ml) and N-Phenylthiourea (PTU) (0.045% stock-Sigma Aldrich) which was circulated with a peristalsis pump (Harvard Apparatus) ...
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bioRxiv - Microbiology 2023Quote: ... Hemin (0.5 mL/L of a 10 mg/mL stock which was prepared in 0.1 N NaOH; Sigma, Cat. # 51280). All solutions were made in Milli-Q-water unless otherwise noted ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
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bioRxiv - Plant Biology 2023Quote: ... 8-day-old plants (n=15) were dipped for 8 minutes in Lugol staining solution (0.1% I, 1%KI, Sigma-Aldrich) and observed in an Eclipse E200 Microscope (Nikon ...
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bioRxiv - Microbiology 2023Quote: ... either alone or in the presence of 22 μL-enzymes cocktail constituted by 1U Cellulase (product n° 22178, Millipore-Sigma), 1U Beta-glucosidase (product n° 49290 ...
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bioRxiv - Microbiology 2023Quote: ... either alone or in the presence of 22 μL-enzymes cocktail constituted by 1U Cellulase (product n° 22178, Millipore-Sigma), 1U Beta-glucosidase (product n° 49290 ...
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bioRxiv - Neuroscience 2023Quote: ... were placed in the middle of a 2% agar plate containing a container with 10 µl n-amylacetate (AM, diluted 1:50 in mineral oil; SAFC) or 3-Octanol (3-Oct, Sigma) on one side and a blank on the other side ...
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bioRxiv - Neuroscience 2023Quote: ... or acidic pH 6.5 media (26mM NaHCO3, 20mM 2-(N-morpholino) ethanesulfonic acid (MES)(Millipore Sigma, St. Louis, MO, USA), 5mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... were plated one day prior to the coculture with γδ T cells at a density of 15.000 cells/well in a flat-bottom 96-well plate and treated O/N with 10 μM pamidronate (PAM, Sigma) in complete IMDM or DMEM ...