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Citations for Millipore Sigma :
2801 - 2850 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... then incubated for 1 h in a PBS solution of 1 mM methacrylic acid N-hydroxysuccinimide ester (MA-NHS, Sigma-Aldrich), diluted from a 1 M MA-NHS DMSO stock ...
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bioRxiv - Molecular Biology 2019Quote: Dry samples were resuspended in 300 µL 0.1 N HCl and filtered through 0.45 micron filters (EMD Millipore, Billerica, MA, USA). 5 µL of the filtered extracts was derivatized according to the AccQTag™ Ultra Derivitization Kit protocol (Waters Corporation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then resuspended in 10 ml of PBS supplemented with 2 mM DTSP (3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester dissolved in DMSO, a crosslinking agent from Sigma, MO, USA) and incubated for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... hearts were transferred in 50% glycerol for 1 h and then mounted in anti-fade mounting media solution containing 100% glycerol + 20%(w/v) n-propyl gallate (Sigma P3130).
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... clean up and trypsinisation was performed on 50-100 mg of frozen tissue (n=4/group) was homogenised in 500 μL CellLytic MT cell lysis buffer (Sigma, C3228) prior to removal of lipid and other contaminants using the ReadyPrep 2D cleanup Kit (Biorad ...
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bioRxiv - Physiology 2020Quote: ... before deproteinization with 1 N perchloric acid (final concentration 0.5 N) and the supernatant collected and filtered Rhrough a 0.22 μm filter (Millex-GV filter; Millipore Sigma, Burlington, MA). Filtered samples were separated using a 50 x 3 mm column (length x i.d ...
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bioRxiv - Physiology 2020Quote: ... physiological responses of the animals were analyzed with an intraperitoneal administration of 1 mg/kg of clozapine-n-oxide (CNO, Sigma-Aldrich) as described above with some modifications ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was chopped into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated with staining solution (3.5 µl/ml nitro blue tetrazolium, N-6876 Sigma; 3.5 µl/ml BCIP, B-8503). After staining for 1-2 h (depending on the probe) ...
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bioRxiv - Molecular Biology 2021Quote: ... n = 3 for each group) were intraperitoneally injected with lipopolysaccharide (LPS; Escherichia coli 0111:B4, Sigma-Aldrich, St Louis, MO, USA), polyinosinic:polycytidylic acid [poly(I:C) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Compounds with VIP>1 and adjusted p-value<0.05 were tentatively annotated by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed and injected into mice ovary orthotopically (n=8) followed by Doxycycline (2mg/ml) and 10% (w/v) sucrose (Sigma-Aldrich) in drinking water after 1 weeks of cells inoculation ...
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bioRxiv - Neuroscience 2022Quote: ... we treated 1 or 14 days primary cultures of hippocampal neurons O/N with 15 mM L-glutamic acid (LGA; Sigma-Aldrich). Total RNA concentration and purity (260/280 and 260/230 ratios ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Bioengineering 2022Quote: ... The nanopatterned flexible films were then washed with an amine-terminated poly(N-isopropylacrylamide) (pNIPAM) solution (13 µM in H2O, Mn = 2500 Sigma-Aldrich) for 24 hours on a tabletop rocker (55 rpm ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 or TFCP2L1 in the case of those imaged for confocal analysis ...
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bioRxiv - Immunology 2022Quote: ... Sections were then cleared using 10% w/v CHAPS and 25% v/v N-Methyldiethanoamine in PBS for 48hrs before washing with PBS followed by 72hrs of blocking 5% donkey serum (Sigma, #D9663) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... and at various times they were treated with 10 mM N-ethylmaleimide (NEM; Sigma-Aldrich, cat. No.-E3876, St Louis, MO) for 5 min at room temperature followed by fixation with 4% paraformaldehyde (PFA ...
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bioRxiv - Plant Biology 2022Quote: ... NEROSs-RFP-Flag and GFP were transiently expressed in 4-week-old tobacco (N. benthamiana) leaves and the expression was induced using 100 μM β-Estradiol (cat. no: E8875-1G; Sigma). Leaves were dark-adapted for 20 min before chlorophyll fluorescence and ETR were measured at 20°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CFP was cut into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: GANT61 (2,2’-[[dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis[N,N-dimethyl-benzenamine) is a GLI antagonist that was used to reduce the number of oligodendrocytes.58,59 GANT61 (Sigma-Aldrich, MO) was dissolved in 100% DMSO to create 10 ...
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bioRxiv - Cell Biology 2022Quote: ... and 50ng/mL nerve growth factor (Alomone, cat. no. N-100) in poly-D-lysine (Sigma, CAS number 27964-99-4) / laminin (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized (n=6) and treated with vehicle (10% D-a-tocopherol polyethylene glycol 1000 succinate in PBS, Sigma Aldrich) or VE822 (60 mg/kg ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Physiology 2024Quote: ... groups of mixed staged adult Hypsibius exemplaris (n≈20 animals) were collected from culture dishes (at 20°C) via manual microaspiration (a Sigma-Aldrich A517 aspirator tube assembly with a hand-pulled 150mm Glass Pasteur Pipette tip ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Cell Biology 2023Quote: Tubulin was purified from porcine brain as described before (Castoldi and Popov, 2003) and labeled with biotin-N-hydroxysuccinimide (NHS; Sigma-Aldrich) or ATTO-647N-NHS (ATTO-TEC ...
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
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bioRxiv - Cancer Biology 2024Quote: The interaction between CAMKV and CREB was assessed in SK-N-AS cells using an in situ PLA assay (Duolink in situ red starter kit, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Molecular Biology 2023Quote: ... the mouse anti-DCP1A antibody was covalently conjugated to a 488 fluorophore using the Mix-n-Stain CF488A Antibody labeling kit (Sigma, MX488AS100) and used together with the rabbit polyclonal anti-ORF1p antibody covalently conjugated to a 647 fluorophore with the Mix-n-Stain CF647 Antibody labeling kit ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then delipidated with clear unobstructed brain imaging cocktail and computational analysis (CUBIC)-L clearing solution (10 wt% N-buthyldiethanolamine [[#471240, Sigma-Aldrich, Germany] and 10 wt% Triton X-100 [Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... we weakened N-cadherin-mediated cell-cell adhesion of SMCs [49] by using the medium with the addition of mouse monoclonal anti-N-cadherin antibody (10 µg/mL, C3865, Sigma-Aldrich). The SMCs were cultured for 48 h on trough-shaped concavity arrays ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM, Sigma) in PBS with 1x cOmplete™ protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... Each group (n = 6) was immunized intraperitoneally with peptides-KLH (10 μg/mouse) emulsified in Incomplete Freund’s Adjuvant (Sigma, Cat.#F5506), and a control group (n = 6 ...
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested in lysis buffer supplemented with protease and phosphatase inhibitors and the isopeptidase inhibitor N-ethylmaleimide (NEM) (Sigma: 04259). After clarification by centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: To establish folic acid (FA)-induced nephropathy, mice (25-30g, n= 6-10 mice per group) were injected intraperitoneally with FA (F7876, Sigma-Aldrich, dissolved in 0.3M sodium bicarbonate at a dose of 250 mg/kg) ...