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Citations for Millipore Sigma :
2751 - 2800 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...
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bioRxiv - Microbiology 2021Quote: ... which were then derivatized by adding 100 μl 90% N,O-bis(trimethylsilyl)-trifluoroacetamide-10% tetramethylsilane (TMS) (Sigma, St. Louis, MO) and 200 μl anhydrous pyridine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... EPR spin-trapping studies were performed using the spin-trapper 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) (Sigma, St. Louis, MO) at 10 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Cancer Biology 2022Quote: ... The solution was neutralized using sodium acetate pH 7.0 and DNA sample was transferred to Whatman Hybond N+ Blotting Membrane (Millipore Sigma, Z761079). Nucleic acids were cross-linked by to the membrane by baking at 60 °C for 30 min followed by UV exposure ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Plant Biology 2022Quote: Proteasome activity was measured using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin (Millipore Sigma; S6510) as described Üstün et al ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Cysteine containing phosphopeptide was conjugated to carrier protein Limulus hemocyanin using hetero-bifunctional crosslinker m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (Sigma# 803227). This conjugate was used to immunize New Zealand white rabbits (Charles River Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... The top flow channel was perfused with hECSR and the bottom chamber with N-Formylmethionyl-leucyl-phenylalanine (fMLP, 10 nM, Sigma Aldrich), a potent neutrophil chemoattractant ...
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bioRxiv - Cancer Biology 2022Quote: ... Actin (#A1978) and DCLK1 (targeted to the N-terminus, #62257) (#SAB4200186) were purchased from Sigma Aldrich (Sigma-Aldrich, St. Louis, MO). Bax (#5023) ...
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bioRxiv - Immunology 2021Quote: ... Monomers were loaded with 0.2 mg/ml peptide at 37°C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma-Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were treated with 0.8 M mannitol [32] with 0.1% (w/v) Calcofluor white (Sigma-Aldrich, cat. n. 18909) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3’-phosphoadenosine-5’-phosphosulfate (PAPS), and [1,2,6,7-3H(N)]dehydroepiandrosterone ([3H]DHEA, 60 Ci/ mmol) were purchased from Sigma-Aldrich (St. Louis,MO). SDS–polyacrylamide gel electrophoresis reagents were obtained from Bio-Rad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Neuroscience 2021Quote: ... catalog #96121229) were cultured as described (N. J. Ortner et al., 2020) in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, catalog #D6546) that was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: U87 glioblastoma (ATCC HTB-14) and SK-N-BE(2) neuroblastoma (ATCC CRL-2271) were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (Sigma-Aldrich, Australia) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Desiccated tissue sections mounted on ITO glass slides were sprayed using an HTX TM-Sprayer (HTX Technologies, LLC) with 10 mg/ml N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC, Sigma #222488) dissolved in 70%:30% methanol:water ...
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bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
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bioRxiv - Cell Biology 2022Quote: ... Pigmentation was prevented during experiments by treating the embryos with 0.002% N-phenylthiourea (PTU) (Sigma-Aldrich, St. Louis, US-MO, #P7629) from 1dpf onwards ...
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bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescent spectra was then acquired after VK3 and NAC (N-acetylcysteine) treatment or auranofin and buthionine sulfoximine (both Sigma, Burlington, MA) treatment or BBI-608 (Boston Biomedical ...
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bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mM EDTA, 150 mM NaCl, 2.5% SDS, 10 mM N-ethylmaleimide, 5 μl.ml−1 protease inhibitor [P9599, Sigma-Aldrich], 100mg PVPP). Samples were mixed gently for 10 minutes at room temperature and centrifuged at 16,000 xg for 1 minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Biophysics 2019Quote: The tetra-p-tosylate salt of meso-tetrakis(4-N-methyl-pyridyl)porphine (TMPyP4+, Fig. 1) and potassium salt of poly(rA) from Sigma-Aldrich Chemical Co ...
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bioRxiv - Biochemistry 2019Quote: ... trimethylsilyl-N-methyl trifluoroacetamide (MSTFA) and N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) were obtained from CS-Chromatographie Service and pyridine from Sigma-Aldrich at >99.98% purity ...
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bioRxiv - Genomics 2019Quote: ... The dried extract was then resuspended in 5 μl of a hexane solution containing 7.5 ng/μl of n-dodecane (EMD Millipore Corp.) as an internal standard ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... 100 μL aliquots of 50 μM K311Dha in in 20 mM NaPi (pH 8) was reacted with 1000 molar equivalents of N-acetylcysteamine (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1 μg/μl) were isolated and solubilized on ice for 5min using 1% β-DDM (n-Dodecyl β-D-maltoside, Sigma) in a buffer containing 0.7 M Sucrose ...
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bioRxiv - Microbiology 2021Quote: ... cadavers or conidia were extracted in 2-mL vials with 500 µL n-hexane (for liquid chromatography LiChrosolv®, Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μL of the NaOH/DMSO slurry was added to the lyophilized N-glycans in addition to 100μL iodomethane (Sigma-Aldrich, #289566) and placed in on a vortex shaker for 20 minutes at room temperature with a microtube cap to prevent the lid from opening due to increased gas pressure ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then lysed under denaturing conditions using the lysis buffer (40 mM HEPES KOH, pH 7.4, 1% SDS) containing 5 mM N-ethylmaleimide (NEM, Sigma-Aldrich, E1275) to prevent deubiquitination and boiled for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... filled with pre-heated (to 37 °C) CUBIC-L solution (10% w/w N-butyldiethanolamine, 10%w/w triton X-100, in H2O, Sigma Aldrich) and kept at 37 °C under continuous shaking ...
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bioRxiv - Neuroscience 2021Quote: ... mediated knockdown of Zfp804a (the rat homolog of ZNF804A) in rat primary cortical neurons was conducted using the N-Ter Nanoparticle siRNA Transfection System (Sigma: N2788) at DIV15 per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...