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Citations for Millipore Sigma :
2901 - 2950 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... BMDM were treated with either 5μm DPC333 (Qian et al., 2007) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes ...
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Versatile and Robust method for Antibody Conjugation to Nanoparticles with High Targeting EfficiencybioRxiv - Biochemistry 2021Quote: ... N-(3-dimethylaminopropyl)-N’-ethyl-carbodiimide (EDC, 97%), Dimethyl sulfoxide (DMSO, >99,5%) and 3D Petri Dish ® - Microtissues were purchased from Sigma Aldrich (Germany). Dulbecco’s modified eagle medium (DMEM) ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Immunology 2020Quote: ... embryos collected from rpe65a:nfsB-eGFP outcrosses were incubated in system water treated with 1.5X n-phenylthiourea (PTU; Sigma-Aldrich, St. Louis, Missouri) from 0.25 – 5 days post-fertilization (dpf ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1:100 dilution of GlutaMax and penicillin/streptomycin and cells were treated (n = 3 per group) with compounds and tBHQ (Sigma 11294-1) for 24 hr ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...
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bioRxiv - Physiology 2022Quote: ... a sharp glass pipette (tip inner diameter: 15–30 μm) filled with NMDA (N-methyl-D-aspartate, Sigma-Aldrich, M3262, 0.2 mM), C21 (200 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % sodium deoxycholate, 0.1 % SDS, 1X protease inhibitor (GenDEPOT, P3100), 1X phosphatase inhibitor (GenDEPOT, P3200) and 50 mM N-ethylmaleimide (Sigma-Aldrich, E3876-5G) using a Dounce homogenizer ...
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bioRxiv - Physiology 2022Quote: ... Protein contents of mitochondrial pellets were extracted by adding 4X volume of 1x MAS buffer followed by 1/9 volume of 10% n-dodecyl β-D-maltoside (DDM; Sigma D4641) for a final 1% DDM concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... from the free radioactivity by transferring the cell suspension to a 0.4 mL centrifuge tube (USA Scientific, Ocala, FL) containing a layer of oil consisting of 1:37.5 n-dodecane:bromo-dodecane (Sigma–Aldrich, St. Louis, MO) and spinning at 12,535 g for 8 s (Beckman E centrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... embryos from all genotypes (N = 7 per genotype) were collected at 40 hpf and fixed with 4% paraformaldehyde (PFA; Sigma, Gillingham, UK) in phosphate buffered saline (PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated in 70% EtOH for 1 hour on a nutator at RT and rinsed in demi-water and stained overnight (O/N) in carmine solution (1g carmine (Sigma, cat. #C1022), 2.5 g aluminum potassium sulphate (Merck ...
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bioRxiv - Systems Biology 2022Quote: ... The extracts were dried under a stream of nitrogen gas and derivatized by incubation at 70 °C for 30 min with 100 μl of N,O–Bis (trimethylsilyl) trifluoroacetamide (BSTFA) and 100 μl anhydrous pyridine (Sigma, St. Louis). After the derivatization reaction ...
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bioRxiv - Physiology 2023Quote: ... eNOS-independent relaxation was tested in presence of the pharmacologic eNOS inhibitor N-nitro-L-arginine methyl ester (L-NAME, 300 μM; Sigma-Aldrich N5751) either using increasing concentrations of the NO-donor dimethylamine nonoate (DEA-NONOate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were resuspended in cold Ripa buffer supplemented with 1% n-Dodecyl-ß-D-maltoside and complete ethylenediaminetetraacetic acid (EDTA)-free protein inhibitors (Sigma-Aldrich), and incubated at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were intravitreally injected with 1.5 μl of 20 mM N-methyl-d-aspartic acid (NMDA) (Sigma-Aldrich, St. Louis, MO). The 1.0mg/mL commercially available morphine solution (M-005-1ML in methanol ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Biochemistry 2023Quote: ... higher temperature and/or purification of the reaction mix did not yield a more homogenous sample as several screening datasets were collected and analysed.) Incubation with n-Octyl-β-D-glucopyranoside (Sigma Aldrich) at a final concentration of 0.1 % (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... 2E9 (epitope as determined by Willem et al.: PWHSFGADSVP, N-terminal of β-cleavage and C-terminal of η-cleavage site; Millipore), M3.2 (mouse Aβ region-specific ...
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bioRxiv - Biochemistry 2023Quote: ... dried phosphopeptides from each fraction were re-dissolved in 800 μL cold IAP buffer (50 mM Tris-HCl, 150 mM NaCl, 1% w/v n-Octyl-β-D-glucopyranoside (EMD Millipore), EDTA-free Halt protease inhibitor and phosphatase inhibitor cocktails ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were visualized by TIRF-M in imaging media and stimulated with a final uniform concentration of 20 nM fMLF (N-Formyl-L-methionyl-L-leucyl-L-phenylalanine, Sigma, cat#F3506) to drive PI(3,4,5)P3 production.
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bioRxiv - Immunology 2023Quote: ... and an N-terminal NusA fusion separated by a TEV cleavage in a pET-50b (+) was transformed into tuner (DE3) (Novagen; cat # 70623), BL21(DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... A 3X proteinase buffer was prepared (For 1 ml: 300 µl PBS, 300 µl 20% N-Lauryl sarcosine sodium solution (Sigma Aldrich, L7414), 60 µl 0.5 M EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... Live-cell AIS staining was performed using a mouse anti-neurofascin antibody targeting an extracellular epitope (NeuroMab clone A12/18, #75-172) conjugated with CF647 (Mix-N-Stain CF647 antibody labeling kit, Sigma-Aldrich #MX647S50) following the manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µM of the nonspecific nitric oxide synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME [Sigma Aldrich], n=5), or 1.0 mM P188 and 100 µM L-NAME (n=5) ...
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bioRxiv - Neuroscience 2024Quote: ... An antibody reactive for the N-terminal amino acids 66-81 of the amyloid precursor protein (APP) (Clone 22C11: Millipore, Billerica, MA) was applied at 1:40K and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
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bioRxiv - Cancer Biology 2024Quote: ... The injected embryos were then incubated at 30°C for 24 - 48 h and allowed to recover in the presence of N-phenylthiourea (Sigma-Aldrich, P7629) to inhibit melanin synthesis ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was cut across the molecular mass region of the corresponding proteins and separately probed with the following corresponding antibodies: an anti-β-glucuronidase (N-terminal) antibody (G5420, Sigma‒Aldrich) and an anti-tubulin antibody (T5168 ...
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bioRxiv - Physiology 2024Quote: ... Plates were washed with 1 M NaCl for 10 seconds and then stained for 5 hours using 0.2% amido black (Sigma Product N- 3393) in 1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2021Quote: ... The effect of metabolites on the thermal stability of SdGA was measured using 0.1 mM acarbose (Sigma-Aldrich cat. n° 56180-94-0), 1 mM CaCl2 or 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Multiple clones were isolated and successful integration of N-terminal 3xFLAG tag was validated by western blotting with anti-FLAG-HRP (Sigma, A8592, 1:10,000).
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bioRxiv - Cancer Biology 2021Quote: ... Purification of N-glycans from the reaction mixture was done on the Supelclean™ ENVI-Carb™ SPE graphitized carbon tubes (Sigma-Aldrich). Prior to purification ...
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bioRxiv - Neuroscience 2020Quote: LC-MS grade water was purchased from Honeywell (Riedel-de Hae n; LOT: I174P) and formic acid was purchased from Sigma-Aldrich (LOT: 179247). LiChromasolv hypergrade Acetonitrile (LOT ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then mounted in 12 µL of mounting media (20 M N-propyl gallate [Sigma-Aldrich] and 0.14 M Tris in glycerol) with a no ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Biophysics 2020Quote: Goat anti-mouse IgG was incubated using a 5 x excess of N-hydroxysuccinimidyl esters – Alexa647 (LifeTech, A20106) and purified after 4 h using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML) to remove excess dyes ...
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bioRxiv - Biochemistry 2022Quote: Labeling buffers were made from lyophilized aliquots of buffer A and were directly resolubilized in D2O (99.9% atom D, Sigma Aldrich P/N 151882) or after adding 6M Urea-d4 (98% atom D ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin, HEPES, GlutaMAX, N-acetylcysteine (1 mM; Sigma-Aldrich, Zwijndrecht, the Netherlands) and 1.5% B27 supplement (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).