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Citations for Millipore Sigma :
2951 - 3000 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Total RNA was extracted from snap frozen tissue (n=10 liver and quadricep muscle) and cells using Tri-Reagent (Sigma-Aldrich, Dorset, UK) and concentration determined spectrophotometrically at OD260 on a Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: Methanol-fixed infected cells and a methanol-fixed negative control were incubated overnight at 4 C° with 150 μl of a solution of anti-S IgG or anti-N IgG in PBS + 3% BSA (Sigma-Aldrich, Milan, Italy). For colocalization experiments ...
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Single cell kinetic modeling of redox-based drug metabolism in head and neck squamous cell carcinomabioRxiv - Systems Biology 2022Quote: ... cells were washed three times with PBS and siRNA packaged in lipid nanoparticles using the N-TER Nanoparticle siRNA Transfection System (Sigma-Aldrich, Cat#N2913) was applied to each well at 50 nM in 100 uL of serum-free media for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... we filtered 500 ml of the collected water through a 0.22 μm Sterivex filter (Millipore Sigma, Burlington, MA; n=4 per time point) using peristaltic pressure ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) into propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Immunology 2020Quote: ... To purify devil MHC-I molecules by immunoaffinity purification, devil cells were expanded (1×109 cells/replicate, n=4) using Corning® HYPERFlask® M cell culture vessels (Sigma-Aldrich), harvested and washed in 1X-PBS before being snap frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... released crypts were mixed with 10ml DMEM Basal Media (Advanced DMEM F/12 containing Pen/Strep, Glutamine, 1mM N-Acetylcysteine (Sigma Aldrich A9165-SG)) containing 10 U/ml DNAse I (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... nitrogen (N) and deuterium (D) isotopes were added: (i) light (L: R0K0) isotopes (12C14N on both Arg and Lys; Sigma-Aldrich: A6969, L8662), (ii ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Physiology 2021Quote: ... dams mated with CON and PHF males (n = 10) were fasted for 6hrs and subjected to a standard intraperitoneal (i.p.) GTT with glucose (G5767; Sigma-Aldrich; 1.5g/kg, i.p.). In another cohort at term gestation (E18.5) ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2020Quote: The sequences encoding mature VdAve1 and VnAve1 were cloned into pET-15b with an N-terminal His6 tag sequence (Novagen, Madison, WI, USA) (primer sequences ...
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bioRxiv - Neuroscience 2019Quote: ... Pregnant Wistar rats (293.0–428.7 g) therefore received a single intraperitoneal (i.p.) injection of either poly (I:C) (n=8; P9582, potassium salt, Sigma-Aldrich; Gillingham, Dorset, United Kingdom) at a dose of 10 mg/kg or 0.9% non-pyrogenic sterile saline as a control (n=8 ...
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bioRxiv - Neuroscience 2019Quote: ... they were dissociated with trypsin and cultured on sterile 5mm n.1 glass coverslips (Warner, 640700) treated with 1mg/ml Poly-D-Lysine (Millipore, A-003-E) and 1mg/ml laminin (Corning ...
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bioRxiv - Physiology 2020Quote: Bone marrow from Winnie and control mice tibiae (n=4-8 mice/group) was collected by flushing with α-modified essential medium ((α-V1EV1, Sigma, Australia). Bone marrow-derived mesenchymal stromal cells (BM-MSCs ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... a subset of mice received an intraperitoneal glucose tolerance test (n=26) using 1.5 g/kg body weight D-glucose (Sigma, St Louis, MO, USA). At the completion of the experiment (8 weeks post-transplant) ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were harvested at E11.5 and N=3 WT and mutant forelimbs (pooled left and right) were dissociated into single cell suspensions using collagenase (Sigma C9697; 50mg/mL) at 37°C for 15 minutes followed by gentle pipette mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Neuroscience 2022Quote: To prepare cell nuclei on the day of library preparation frozen tissue was promptly transferred from -80 °C storage to a chilled 2mL dounce with chilled Nuclei EZ Lysis Buffer (Sigma-Aldrich N 3408). Sample was dounced 25 times with pestle A and 20 times with pestle B ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Immunology 2022Quote: ... were dosed 2 mL/Kg intraperitoneally (IP) twice weekly with either olive oil (N=8) or CCl4 (CCl4:olive oil, 1:3, [v:v], Sigma-Aldrich, St Louis, MO) for 4 weeks ...
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bioRxiv - Microbiology 2024Quote: ... and influenza A/California/7/2009 H1N1pdm09 virus were amplified on MDCK α2-6 in MEM supplemented with 1% HEPES and 1 μg/ml trypsin treated with N-tosyl-L-phenylalanine chloromethyl ketone (Sigma, Oakville, ON, Canada). Viral titers were determined by plaque assays ...
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bioRxiv - Neuroscience 2024Quote: ... sections from a subset of animals (n=16) were processed identically as described above with rabbit anti-nNOS (Millipore Cat# AB5380, RRID AB_91824) and the appropriate secondary antibody conjugated to Alexa Fluor 647 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates (intracellular RBD and N protein) were generated by processing the cell pellets in a suitable volume of RIPA buffer (Sigma Aldrich cat# R0278) according to the manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... see the Results) and a second egg with the 10 µl of PBS (Sigma Aldrich, Cat. No. D5652-50L; E13-PBS, N = 53), serving as a control ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOC) and N,O-bis(trimethylsilyl)trifluoroacetamide (TMS) (Sigma-Aldrich, St. Louis, MO, USA). The dried extracts were reconstituted in 60 μL of 11.4 mg/mL MOC in anhydrous pyridine (VWR International ...
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bioRxiv - Biochemistry 2022Quote: ... only C501 and C527 of C2-TRAP1 variant) were blocked by incubating proteins with 10 mM N-ethylmaleimide (NEM, Sigma-Aldrich, Missouri, USA) for 30 min at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... The filtered sample was loaded at 0.5 mL/min onto an AKTA FPLC system fitted with either a Mono S 5/50 GL column or freshly prepared 5 mL fine mesh cellulose phosphate column (Sigma P/N C2258) equilibrated in binding buffer containing 50 mM HEPES (pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
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bioRxiv - Systems Biology 2024Quote: ... Abs were then reacted with 10x molar excess of dibenzocyclooctyne-S-S-N-hydroxysuccinimidyl (DBCO-S-S-NH) ester (Sigma Aldrich, 761532-1mg) for 2 hours at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG2000, Laysan Lipids, Arab, AL) were dissolved in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... was electroblotted (25 V and 1 mA for 20 min) under semi-dry conditions onto a positively charged Hybond®-N+ hybridization membrane (Millipore Sigma, GERPN203B) using a Trans-Blot® TurboTM Transfer System (Biorad ...
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bioRxiv - Microbiology 2023Quote: ... 0.165 M 3-(N- morpholino)propanesulfonic acid (Melford, Suffolk, UK) adjusted to pH 7 by 1 M NaOH (Sigma-Aldrich, Burlington, MA, USA)) ...
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Neuroscience 2024Quote: ... mice were intra-amygdalally (i.a.) injected (ventral [V], −2.0 mm below the guide cannula) with either kainic acid (i.a.KA, Sigma-Aldrich, USA; 0.3 µg/2µL; n=30) to induce status epilepticus or sterile phosphate-buffered saline (i.a.PBS ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Physiology 2022Quote: ... cells were washed two times with PBS+/+ and medium was replaced with glucose-free DMEM (catalog n°D5030, Sigma-Aldrich, Saint-Louis, MO, USA) without phenol red supplemented with 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... and kept for 60 s at pH 7.4.The mixture was digested with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 200 μg/mL in the presence of 1% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a sub-set of benzene and air-exposed mice (n=24/group) were treated with 0.5 mg/kg lipopolysaccharides (LPS, Sigma Cat# 2630, Lot# 028M4022V; i.p). At the end of the exposure protocol ...