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Citations for Millipore Sigma :
2601 - 2650 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...
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bioRxiv - Biophysics 2022Quote: Myristoylated c-Src constructs were obtained by co-expression of the N-myristoyl transferase (NMT) enzyme along the c-Src USH3-His6Tag construct in a pETDuet-1 (Novagen) plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: Live TTX-treated WT and Fmr1R138Q hippocampal neurons (14-15 DIV) were incubated with a mouse monoclonal anti-N-terminal GluA1 antibody (1:100; Merk-Millipore) for 10 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... Surviving midges and mosquitoes at day 10 were pooled (n=5-10) in 1 ml virus transport media (199E media supplemented with antibiotic-antimycotics; Sigma), and stored at −80°C until processed for virus isolation (VI ...
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bioRxiv - Microbiology 2020Quote: ... virus containing media was transferred to a new tube and the cell pellet was lysed in 500 μl of lysis buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1% n-dodecyl β-D-maltoside, 1X protease inhibitor (Sigma)) ...
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bioRxiv - Neuroscience 2019Quote: ... coated glass slides in a 12-well plate O/N and then were stressed with 0.25 mM Sodium Arsenite (NaArs, S7400-100G Sigma-Aldrich) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: Apoptotic cells were revealed in 2-days-old live embryos by adding the Acridine Orange vital dye (N-4638; Sigma) to the embryo water at a final concentration of 5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... and derivatized by the addition of 150 μL of pyridine and 150 μL of N,O-bis(trimethylsilyl)trifluoro-acetamide with trimethylchlorosilane (99 : 1, w/w, Sigma) and incubated at 70 °C for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled samples were washed 3 times in PBT (10 min each) and incubated with DAPI (Sigma, cat. N. D9542) (5μg/ml ...
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bioRxiv - Physiology 2021Quote: ... IF n = 7 pups per sex from N = 7 litters) under Inactin anaesthesia (sodium thiobutabarbital 100 mg/kg body weight i.p., T133, Sigma Aldrich), as described previously [17] ...
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bioRxiv - Neuroscience 2022Quote: ... allele-specific PCRs were performed on 2 µl crude buccal DNA in a total of 10 µl using the REDExtract-N- AmpTM PCR ready MixTM (Sigma) according to the manufacturer’s protocol using 0.5 µM forward ...
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bioRxiv - Neuroscience 2022Quote: ... mice were given ad libitum access to 5 mg of clozapine-N-oxide (CNO; Sigma-Aldrich cat. #34233-69-7) dissolved in 200 mL of drinking water.
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bioRxiv - Microbiology 2022Quote: ... Nine biological replicates (n = 9) of seven-day-old seedlings were transferred to ⅕ MS agar plates supplemented with 0.1 mM serotonin (Sigma-Aldrich) or with purified H2O ...
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bioRxiv - Cancer Biology 2022Quote: ... To covalently conjugate the DNA strands to the surface aldehyde groups—thereby converting the hydrolysable Schiff base to single C─N bonds—reductive amination was conducted at room temperature for 15 min in 0.25% sodium borohydride (Sigma-Aldrich) in 1× phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... was mixed into the prepolymer solution and before adding 0.005% w/v acrylic acid N-hydroxysuccinimide (previously dissolved in DMSO, Sigma-Aldrich), pH of the solution was neutralized with HCl (0.0054 % 1 M ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Systems Biology 2019Quote: ... Radical formation in HepG2 cells was measured by ESR spectroscopy in combination with the spin trapping technique using 50 mM 5,5-dimethyl-1-pyrolline N-oxide (DMPO, Sigma-Aldrich). Protein oxidation was confirmed via a protein carbonyl assay and oxidative DNA damage via detection of this using the Fpg comet assay (Deferme et al ...
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bioRxiv - Systems Biology 2019Quote: ... 0.01 g/l CaCl2 ×2H2O) were supplemented with 5 g/l Peptone N-Z-Soy BL 7 (Sigma-Aldrich S.r.l) (complex medium ...
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bioRxiv - Physiology 2019Quote: ... IHC was performed by incubating sections (n=5 per each group) with the primary antibody (anti-mUCP-1, U6382, Sigma), followed by a secondary antibody conjugated with HRP ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Six μm sections of formalin-fixed paraffin-embedded tissue from the cortex frontal (n=3) were used to stain NeuN (Millipore), IBA1 (Wako) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: ... Next day strains were diluted to an OD600 of 0.05 in fresh glucose M9 medium containing N-acyl homoserine lactone (AHL, Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and lysed in 300 μl of lysis buffer (20mM Tris pH=8.0, 150mM NaCl, 1% Triton) containing 100 μM of N-Ethylmaleimide (Millipore-Sigma) and 1Xprotease inhibitor cocktail (Biotool ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Cell Biology 2019Quote: ... and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore, Tullagreen Carrigtwohill Co.Cork ...
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... Griess reagent was prepared in-house by mixing equal volumes of 0.1% N-(naphtyl)ethelenediamine (NED) (catalog no. 222488; Sigma-Aldrich) in distilled water with 1% Sulfanilamide (catalog no ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Developmental Biology 2019Quote: 6 dpf Cntnap2a-positive fish were treated for 1 hour with 0.05% DASPEI (2-4-dimethylamino-N-ethylpyridinium iodide; Sigma Aldrich), which labels both superficial and canal neuromasts [46] ...
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bioRxiv - Cancer Biology 2019Quote: ... RIPK3 inhibitor GSK 872 (N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine) was purchased from Millipore Sigma and frozen stocks were maintained in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM NaF (Nacalai tesque), 20 mM N-ethylmaleimide (NEM, Nacalai tesque) and 0.25 mM phenylmethylsulfonyl fluoride (PMSF, Sigma-Aldrich). The cells were washed twice with ice-cold PBS and incubated with an appropriate volume of CSK buffer for 1 hour on ice with occasional mixing ...
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bioRxiv - Bioengineering 2021Quote: ... the BMOs were placed in an increasing sucrose solution (7.5 % for 1 h, 15 % for 1 h and 30 % O/N; Sigma, S1888) for improved cryoprotection ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Biochemistry 2021Quote: ... Proximity ligation reaction was followed by O/N decrosslinking at 65°C in the presence of 250 μg/ml proteinase K (Eurobio) and 5 mM EDTA (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Membranes were then solubilized overnight at 4°C in presence of 0.5% n-Dodecyl β-D-maltoside (DDM) (Sigma, D4641). Lysates were used for co-immunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... M and N proteins were concentrated and buffer changed to phosphate buffered saline (PBS) using Microcon-10 KDa centrifugal filter units (Millipore). For N-terminal (residues from 43 to 180 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...
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bioRxiv - Immunology 2021Quote: Elastase activity was determined by the chromogenic substrate N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (M4765, Sigma-Aldrich, USA). Supernatants were used for measuring elastase activity ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... fresh-frozen tissue sections were fixed in 4% paraformaldehyde in HBSS overnight at 4°C and treated with 0.2 N HCl for 20 minutes and subsequently with 3 μg/ml Proteinase K (#3115887001, Sigma-Aldrich) for 7 minutes at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole, pH 8.0) to 2 mg/mL, and 0.6% n-Dodecyl β-D-maltoside (DDM, w/v) (Sigma-Aldrich) and 0.01% lysophosphatidylcholine (lyso-PC ...