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Citations for Millipore Sigma :
2501 - 2550 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Ifngr1flox/flox male mice (n=4/group) were daily administrated intraperitoneally with tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) solution (20 mg/mL stock solution dissolved in corn oil (Aladdin ...
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bioRxiv - Cancer Biology 2024Quote: ... Larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich) to prevent pigment formation during imaging experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941). After vortexing for 1 min samples were retuned to −80°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Microbiology 2023Quote: ... Pelleted cells were resuspended in 1X PBS with 100 mM N-ethylmaleimide (NEM) (Cat#128-53-0, Millipore Sigma) for 15 minutes at room temperature27 ...
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bioRxiv - Biophysics 2022Quote: ... 1% DDM [n-Dodecyl-β-D- maltoside]) containing cOmplete mini protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Lysates were mixed 1:1 with 2X LDS sample buffer containing 50 mM TCEP (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were then mixed with freshly prepared Griess solution consisting of 0.1% N-1-naphthylethylenediamine dihydrochloride (Sigma, #N9125) and 1% sulfanilamide (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: Neuroblastoma cell line SK-N-AS was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: PAW-induced outer membrane permeability was measured based on fluorescent dye N-phenyl-1-naphthylamine (NPN; Sigma-Aldrich, Australia) uptake [22] ...
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bioRxiv - Biochemistry 2023Quote: Purified LceB was dialyzed O/N at 4 °C with the addition of 1:200 mg/mg LceB:thrombin (Sigma) to remove the N-terminal 6His-tag ...
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bioRxiv - Microbiology 2023Quote: ... 1x106 ATCC46645-H2A:mRFP and ATCC46645-H2A:mRFP Bir1OE-N conidia were inoculated in 100 mL of RPMI + 0.5 ug/mL Voriconazole (Sigma-Aldrich) and incubated at 50 RPM for 6 hrs at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... and microforged glass capillaries (25 to 30µm inner diameter) filled with mineral oil (ref. n°M5904, Merck/Sigma-Alrich). 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx. ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... consisting of PBS supplemented with 1mM of the amino-reactive MA-NHS (Methacrylic Acid N-Hydroxysuccinimide Ester, Sigma-Aldrich). Specimens were incubated for 1-1.5 hours on gentle rocking at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... The DNA corresponding to the fxn gene was amplified using KOD hot start master mix (Cat. N° 71842 Millipore) and FwDdFXNOE and RevDdFXNOE as primers (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... 10-200 mg tissue was digested in 1 ml 3 N hydrochloric acid (Fisher)/10% trichloroacetic acid (Millipore Sigma) at 65 °C for two days ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from mouse tail tip samples using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... we deployed a rapid lysis protocol using reagents from the SYBR Green Extract-N-Amp Tissue PCR Kit (Sigma) (Doan and Monuki ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 % (v/v) n-dodecyl-β-D-maltoside (DDM from Inalco Pharmaceuticals) and 1.6 mg/mL iodoacetamide (Sigma-Aldrich) for 20 min at 4 °C ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: Animals were randomized with the sealed envelope method to receive either rapamycin (Sigma Aldrich, 250 μg/kg, n=9) or vehicle (<5% ethanol in saline ...
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bioRxiv - Physiology 2023Quote: ... Second derivatization was with 35 µL N,O-Bis(trimethylsilyl)trifluoroacetamide (15238- 10x.1ml, Sigma-Aldrich, St. Louis MO) on thermo-shaker at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and lysed in NP-40 buffer supplemented with 10 mM N-ethylmaleimide (Sigma, catalog no.: E3876). Flag-immunoprecipitations were performed as described previously ...
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bioRxiv - Neuroscience 2023Quote: ... The antennae were bent outwards from the head and stabilized by a small droplet of n-eicosane (Sigma Aldrich) onto the edge of a microscopy glass slide ...
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bioRxiv - Microbiology 2023Quote: ... Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day) 0.22 µm Sterivex polyethersulfone membrane filter unit (Millipore) to obtain biomass for DNA extraction and following amplicon sequencing of 16S and 18S small subunit ribosomal RNA (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reconstituted visual pigments were extracted using 1% n-dodecyl b–D-maltoside (DDM) (Sigma-Aldrich, Saint Louis, MO) in 10 ml of extraction buffer (pH 6.7 ...
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bioRxiv - Biochemistry 2023Quote: ... Tubulin was further purified by recycling and some tubulin was labeled with Alexa647-N-hydroxysuccinimide ester (NHS; Sigma-Aldrich), Atto647-NHS (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (10 µg) was separated by denaturing agarose gel electrophoresis and transferred onto Hybon-N+ membrane (Sigma-Aldrich) and probed with [32P]dCTP-radiolabelled DNA fragments ...
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bioRxiv - Cell Biology 2024Quote: ... reactions were diluted in 500 µl immune-precipitation buffer (RIPA buffer supplemented with 40 mM N-Ethylmaleimide (Sigma-Aldrich), 40 mM iodoacetamide (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Some vessel segments were incubated with L-NAME (N-nitro-L-arginine methyl ester 100 μmol.L-1, Sigma-Aldrich), The mechanical activity was isometrically recorded using a force transducer and a data acquisition system (DATAQ Instruments ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated o/n (4°C) with primary antibody against Laminin (Rabbit polyclonal, Sigma, L9393, diluted 1:20). Alexa Fluor Donkey anti-Rabbit 488 (A11034 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing N-cTnC were pooled and concentrated using Amicon centrifugal concentrators with a 3 KDa molecular weight cut-off (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... 25 mM HEPES pH 7.4, 1 mM EGTA and 30% v/v Percoll, cat n. GE17-0891-01, Sigma-Aldrich) in a 10-ml polycarbonate ultracentrifuge tube ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Developmental Biology 2021Quote: ... E11.5 embryos were dissected into ice-cold PBS and associated yolk sacs used for rapid genotyping using the Extract-N-Amp Tissue PCR kit as recommended by the manufacturer (Sigma). During genotyping ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) [both purchased from Sigma] ...
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was extracted from single blastocysts by placing each blastocyst in a microtube containing 4.4 µl Extraction buffer (REDExtract-N-Amp Tissue PCR Kit, Millipore Sigma) mixed with 1.1 µl of Tissue Prep Buffer ...