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Citations for Millipore Sigma :
2451 - 2500 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 100 μM hydrogen peroxide (H2O2) for 5 min or alkylated with 20 mM N-ethyl maleimide (NEM; Sigma #E3876) in 100 mM sodium phosphate ...
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bioRxiv - Cell Biology 2021Quote: ... saturated 1 hr in 4% BSA and incubated O/N with rabbit anti-Laminin antibody (1:300, Sigma-Aldrich) to reveal myofiber outlines ...
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bioRxiv - Cell Biology 2022Quote: Representative slices from different areas of the tissue were fixed O/N with 10% neutral buffered formalin (Sigma-Aldrich). Next ...
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bioRxiv - Neuroscience 2020Quote: ... live TTX-treated neurons were incubated with either a mouse monoclonal anti-N-terminal GluA1 (1:40, Merk-Millipore) or GluA2 (1:30 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Biochemistry 2021Quote: ... The residual acetone was removed by brief evaporation and the precipitate was resuspended (600μl) in urea buffer containing 100mM N-ethylmaleimide (d0-NEM) (E3876, Sigma), dissolved in EtOH ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min at 4°C with 22C11 antibody (anti-N-terminus of APP, Millipore) or anti-FLAG (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min at 4°C with 22C11 antibody (anti-N-terminus of APP, Millipore) or anti-FLAG (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Experimental mice of the hM4Di or control group were randomly treated with saline or Clozapine-N-oxide (CNO 3mg/kg i.p. in 0.9% saline, Sigma-Aldrich) and placed for 1 hour in their home cage where the test was subsequently carried out (Figure 2) ...
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bioRxiv - Biochemistry 2021Quote: ... One milliliter of the N-oxide solution was added dropwise to 3 mL of sodium acetate (37.5 mM; pH 5.3; Sigma Aldrich), followed by incubation at room temperature for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: Wasp eggs were collected upon bleeding 50 infested larvae in PBS 1X added with some N-Phenylthiourea crystals (Sigma) to prevent haemocyte melanisation ...
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bioRxiv - Genetics 2022Quote: ... The homogenates were then lysed on ice for 30 min in nucleus lysis buffer (HEPES 50 mM, EDTA 10 mM, N lauryl sarkosyl 0.5%, sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma). DNA was sheared using a Bioruptor pico from Diagenode for 10 cycles (30 sec on ...
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bioRxiv - Immunology 2022Quote: ... cells using minimal essential medium supplemented with 0.2% bovine serum albumin and 2 μg/mL N-Tosyl-L-phenylalanin-chlormethylketon (Sigma). For the determination of the tissue culture infectious dose 50 (TCID50) ...
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bioRxiv - Developmental Biology 2019Quote: ... Denuded fail to fertilize M-II oocytes (n=8) were obtained 36h post-ovulation and cultured briefly (2-4h) in 50µl KSOM medium (Millipore) containing 0.4% BSA at 37°C ...
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bioRxiv - Physiology 2019Quote: ... general Cl− transport was inhibited with 4,4’-diisothiocyano-2,2’-stilbenedisulfonic acid (DIDS, 100 μmol L−1, N = 4) (all chemicals from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... Samples were dried in a vacuum centrifuge and were derivatised by the addition of 0.1mL BSTFA (N,O-Bis(trimethylsilyl)trifluoroacetamide) TMCS (Trimethylchlorosilane) (99:1, Sigma) and 0.3 mL anhydrous pyridine (Sigma ...
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bioRxiv - Neuroscience 2019Quote: DNA from offspring was extracted from mouse ear punches using Extract-N-Amp® kit (Sigma, St. Louis, MO) and genotyped using a multiplex PCR reaction ...
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bioRxiv - Zoology 2020Quote: PCR amplification of two genomic regions were performed using 12.5 μl of the Extract-N-AmpTM Tissue PCR kit (Sigma), 1 μl of each primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... IVM was converted into a fluorescent molecule using N-methylimidazole and trifluoroacetic anhydride (Sigma Chemical, St Louis, MO, USA). An aliquot (100 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended in 200 µL of W-Buffer and treated with 1% SDS (Sigma cat. n° 05030) and 0.1 mg Proteinase K (Euroclone ...
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bioRxiv - Molecular Biology 2020Quote: ... The aqueous phase was filtered with Millipore ultrafree MC tubes and then precipitated with isopropanol (Sigma, cat. n° I9516) at - 80°C for 2 hours or overnight ...
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bioRxiv - Cell Biology 2020Quote: ... and lysed in 300 μl of lysis buffer (20mM Tris pH=8.0, 150mM NaCl, 1% Triton) containing 100 μM of N-Ethylmaleimide (Millipore-Sigma) and 1Xprotease inhibitor cocktail (Biotool ...
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bioRxiv - Developmental Biology 2020Quote: ... pigmentation of embryos was prevented by treating them with 0.002% N-phenylthiourea (PTU) (Sigma-Aldrich, St. Louis, US-MO) starting at 25hpf ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed once with cold PBS containing 20 mmol/L N-ethylmaleimide (NEM, Cat. No. E3876, Sigma-Aldrich) and then lysed in radioimmunoprecipitation assay buffer (25 mmol/L Tris (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... Group 2 (DN+CL, n=8): mice received surgical denervation of BAT and intraperitoneal injections of CL316243 (C5976; Sigma) at 1mg/kg for consecutive 14 days ...
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Cell Biology 2020Quote: ... 10 % glycerol and bromophenol blue) containing 50 μM phenylmethylsulfonyl fluoride (PMSF) and 50 μM N-ethylmaleimide (all Sigma-Aldrich). For LMα3 ECM processing blots ...
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bioRxiv - Microbiology 2021Quote: ... dissolved in PBS or 0.8 mg/ml fMLP 2.5 ml (N-Formyl-Met-Leu-Phe, F3506, Lot#MKCC5355; Sigma-Aldrich), where 2 mg fMLP was dissolved in 50 μl DMSO and then in 2.5 ml PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Clozapine-N-oxide (NIDA Drug Supply Programme) was dissolved first in 5% DMSO (Dimethyl Sulfoxide, Sigma-Aldrich, Poole, UK) and then in sterile 0.9% NaCl (Henry Schein Ltd ...
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bioRxiv - Plant Biology 2020Quote: The ability of pollen to remove OH• (generated in a Fenton reaction) was analyzed in experiments with spin trap DMPO (5,5-dimethyl-1-pylloline-N-oxide) (Sigma). First H2O2 and spin trap were added to a suspension of pollen ...
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bioRxiv - Neuroscience 2022Quote: ... we stimulated cells O/N with 15 mM LGA before incubating them with 2.5 µM calcein-AM (Sigma-Aldrich) and 0.4 µg/mL propidium iodide (PI ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Surface hydrocarbons were extracted from individual eggs and larvae using 10µl of n-pentane (≥99%, HPLC grade, Sigma-Aldrich) for 2 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Purified Vnucfree solubilized in 0.03% n-DDM was mixed with the lipid stock and membrane scaffold protein MSP1E3D1 (Sigma) at a specific molar ratio of enzyme ...
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bioRxiv - Cell Biology 2022Quote: ... and re-solubilized in the working cell buffer with 2.25 mM n-Dodecyl β-D-maltoside (DDM, Sigma Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... paraformaldehyde (using a stock solution with 0.4 g paraformaldehyde dissolved in 10 ml 0.1 M sucrose with 0.6 µl 10 N sodium hydroxide [all from Sigma-Aldrich]), 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the supernatant was incubated O/N at 4°C with 8µg (per condition) of anti-NUP98 (rat, N1038 – Sigma). 62.5µl of A/G beads per condition were added and incubated for 2h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The total membranes were solubilized with a buffer containing 1×PBS (pH 7.4) and 0.5% N-lauroylsarcosine sodium (Sigma-Aldrich) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... but without the use of iodoacetamide for cysteine alkylation due to the treatment of 50 mM N-ethylmaleimide (Sigma) during the sample immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 250 mM xylose or 225 mM N-acetylglucosamine) were applied to a TLC Silica gel 60 F254 (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... 22 mm × 22 mm #1.5 borosilicate coverslips (DOT Scientific) were placed in a Wash-N-Dry coverslip rack (Sigma) and submerged in ∼80 mL of 1 M NaOH in a 100-mL glass beaker ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were collected and raised at either 28.5 or 24°C in N- Phenylthiourea (PTU, Sigma Aldrich, Cat# P7629)/Volvic source water (0.003% final ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... – N medium was prepared using 0.5x MS basal salt micronutrient solution (M0529-1L, Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 1.5mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941). After vortexing for 1 min samples were retuned to −80°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cancer Biology 2024Quote: ... Larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich) to prevent pigment formation during imaging experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The homogenates were then lysed on ice for 30 min in nucleus lysis buffer (HEPES 50 mM, EDTA 10 mM, N lauryl sarkosyl 0.5%, sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma). DNA was sheared using a Bioruptor pico from Diagenode for 10 cycles (30 sec on ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...