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Citations for Millipore Sigma :
2401 - 2450 of 3056 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... culture media was replaced by culture media supplemented with 1 mg/mL MTT reagent (Sigma, cat n° M5655). MTT reduction to formazan crystals by cellular oxidoreductases were allowed to form by incubating the cells in a 5% CO2 gas-equilibrated humidified incubator at 37°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 μg of MurA were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction volume of 50 µl containing 100 mM Tris/HCl pH8.0 and 150 mM NaCl as the buffer and preincubated at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis was then performed by submerging slides in lysis buffer (2% N-Lauroylsarcosine sodium salt (Sigma-Aldrich), 0.5M NA2EDTA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Lysis buffer was used as a positive control of dead cells with tamoxifen (Sigma-Aldrich Cat. N. T5648).
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Plant Biology 2024Quote: 0.1% KMnO4 in 0.1 N H2SO4: Add 0.01g KMnO4 (Sigma-Aldrich, Cat. No. 223468, St. Louis, MO, USA) in 10 mL 0.1 N H2SO4 (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... CD1 male (n=10 per group) mice between the ages of 4-6 weeks were given tamoxifen (Sigma) in peanut oil ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then reduced in 50 mM Tris HCl (pH 8.2) / 2.4 mM sodium deoxycholate / 2.4 mM sodium N-lauryl sarcosine / 5 mM dithiothreitol (all from Sigma Aldrich). Sulfhydryl groups in cysteines were alkylated with 10 mM 2-chloroacetamide (Fluka ...
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bioRxiv - Cell Biology 2024Quote: RSL3 (#SML2234) and S-nitroso-N-acetylpenicillamine (SNAP, #N3398) were purchased from Sigma-Aldrich (St. Louis, MO, USA). CPZ (chlorpromazine ...
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bioRxiv - Cell Biology 2024Quote: Lysis was performed in 4 mL of 0.01 % n-dodecyl-beta-maltoside in 60 mM triethyl ammonium bicarbonate (TEAB, pH 8.5, Sigma) at 95 °C in a PCR cycler with a lid temperature of 110 °C for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... NovaMatrix) was dissolved at 1% w/v in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES; Sigma-Aldrich), 0.3M NaCl (EMD Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... The following chemicals were then added quickly to the 1 wt% alginate solution with the following order: N-hydroxysuccinimide (NHS) (261.6 mg, 2.3 mmol, 0.4 eq., #130672 Sigma Aldrich), EDC (435.5 mg ...
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bioRxiv - Cancer Biology 2024Quote: ... and used to transduce N/TERT-1 keratinocytes with a MOI of 100 via spinfection using Polybrene (Sigma) as transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Microbiology 2020Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre-soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated at 4°C o/n with the monoclonal anti-flag M2 primary antibody (Sigma Aldrich) in TBS-T buffer containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was washed once with buffer A and then resuspended in 10 mM Tris-HCl pH 7.5 + 0.1% N-lauroyl-sarcosin (Sigma-Aldrich) and Complete Protease Inhibitor Complete (buffer B) ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Gelsolin TL40 (N-terminal cytoplasmic gelsolin, Hypermol, Germany) was covalently bound to carboxylated microspheres (0.9 µm diameter, Sigma Aldrich) with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in order to bind the +end of a single actin filament ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells’ membranes was lysed by adding 1 volume of the 2x Lysis Buffer (Buffer N supplemented with 0.6% NP-40 substitute (Sigma)) to the single cell suspension resuspended in 2 PCVs (packed cell volumes ...
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bioRxiv - Neuroscience 2020Quote: ... we selected pentyl acetate (PA; also known as amyl acetate or n-amyl acetate; Sigma-Aldrich, cat no. 109584). EA and PA are homologous esters that differ by a length of three carbons ...
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bioRxiv - Physiology 2022Quote: ... The glucose content of the supernatant was measured using hexokinase (catalog n° H4502, Sigma-Aldrich, Saint-Louis, MO, USA) and glucose-6-phosphatase enzyme (catalog n°G8404 ...
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bioRxiv - Neuroscience 2021Quote: Antibodies and dilutions were as follows: Anti-HNK-1 antibody (Sigma Aldrich, Monoclonal Anti-HNK-1/N-CAM (CD57), # C6680) ...
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bioRxiv - Molecular Biology 2020Quote: Yolk sacs were dissected from embryos and used for DNA extraction with the Red Extract-N-Amp kit (Sigma). Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Retinal lesions were induced by a single intravitreal injection of 0.5 - 1 nL of freshly prepared 100 mM N-Methyl-D-aspartic acid (NMDA, M3262, Sigma). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... a sample was removed and diluted 1:1 into ice-cold PBS containing 20 mM N-ethyl maleimide (Sigma). Cells were collected by centrifugation at 1250 xg for 5 minutes and washed twice with ice-cold PBS ...
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bioRxiv - Bioengineering 2022Quote: ... FDG is mixed in LB to 0.2 mM with IPTG (1 mM, final concentration), sodium N-lauroyl sarcosine solution (0.1%, cell lysate buffer to allow FDG in) (Sigma), Pluronic F127 (0.001%) ...
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bioRxiv - Physiology 2019Quote: ... The study included five treatment groups (n≥6 per group) receiving either 0.24 g/kg bodyweight bovine fetuin-A (Sigma) or 0.10 g/kg bodyweight PPi (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... Labeled standards for indole-3-acetic acid and N-(3-indolylacetyl)-DL-aspartic acid were obtained from Sigma-Aldrich. Calibration curves were linear (r values = >0.99 ...
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bioRxiv - Neuroscience 2019Quote: Female fly heads (30-50 per N) at 2 DAE (days after eclosion) were homogenized using a mechanical pestle homogenizer in 200μl Trizol (Sigma). After adding 800μl Trizol with 200μl chloroform and a 15min centrifugation at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... The glass micropipettes were surface-modified through silanization with N-(2-aminoethyl)- 3-aminopropyltrimethoxysilane (EDA) (Sigma Aldrich catalog # 440302) in order to reduce stiction forces between polysilicon and the wall of the miniaturized glass micropipette during their integration ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were diluted to 17.5 μg/ml in 100 μl 0.1M 2[N-Morpholino]ethanesulfonic acid (MES)-buffer (M2933, Sigma-Aldrich), pH 4.5 ...
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bioRxiv - Cell Biology 2019Quote: ... inhibitors (AR-C155858 – Tocris #4960, LY294002 – Sigma #L9908, Quercetin – Sigma #00200595, Cycloheximide – Sigma #C1988 and N-acetylcysteine – Sigma #A7250) were applied 15 min before lactate or pyruvate stimulation ...
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bioRxiv - Cell Biology 2019Quote: ... larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich). All zebrafish lines used in this study are listed in Table S1.
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated for one minute before blotting with filter paper Whatman n° 1 (Sigma Aldrich, Munich, Germany). Subsequently ...
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bioRxiv - Synthetic Biology 2020Quote: ... Marchantia 5-7 day old sporelings were co-cultivated with 100 µL of Agrobacterium culture in 4 mL of liquid 0.5x Gamborg B5 plus supplements (0.1% N-Z amino A (Sigma C7290), 0.03% L-glutamine ...
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bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were washed 10 times with PBS/Tween and substrate was added (o-phenylenediamine dihydrochloride tablets) and then quenched with 0.2 N sulfuric acid (Sigma) after colour appears visible ...
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bioRxiv - Cell Biology 2020Quote: ... and 2μL of purified gDNA was added to 5μL of the 2X REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich), 0.2μL 10μM forward/reverse primer mix ...
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bioRxiv - Microbiology 2021Quote: ... Gold strain was performed in 800 ml 2xYT o/n at 37°C after induction with 0.5 mM IPTG (Sigma). Cells were harvested by centrifugation ...
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bioRxiv - Bioengineering 2021Quote: ... the particles were transferred into aqueous solution (DPBS or cell media) containing 10µM N-ethylmaleimide (NEM, Sigma-Aldrich, E3876) at a pH of 6.5 ...
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bioRxiv - Bioengineering 2021Quote: ... 2.5-month-old CON male and female (C57Bl/6J, n=7-9/sex/group) were injected with 0.5 mg/kg of raloxifene hydrochloride (SIGMA) for 6 weeks ...
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bioRxiv - Bioengineering 2020Quote: ... O,O′-bis[2-(N-succinimidyl-succinylamino)ethyl]polyethylene glycol with molecular weight of 2kDa (NHS-PEG-NHS, Sigma) was mixed with the protein solution at 10 w/w% ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized for 20 min at RT with DPBS + 0.1% Triton X-100 (Sigma, Cat N° T8787-250ML) followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
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bioRxiv - Neuroscience 2019Quote: ... or a control vehicle (n=11; 5% ethanol, 5% Cremphor EL [C5135-500G; Sigma-Aldrich, Inc.], and 90% saline) 30 minutes before testing ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 200x molar excess of succinimidyl 4-[N-maleimidomethyl]cyclo-hexane-1-carboxylate (SMCC-Sigma-Aldrich #M5525) for 2.5 hours at room temperature to generate maleimide-DNA capable of sulfhydryl-reactive crosslinker chemistry with peptides with terminal cysteines ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...