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Citations for Millipore Sigma :
2001 - 2031 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cell culture medium was then replaced with the PsV-IgG or PsV-MB mixture supplemented with 10 µg/mL polybrene (Sigma-Aldrich, St. Louis, MO). Cells were incubated for 48 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Biochemistry 2021Quote: ... or with anti-GFP primary antibody (1:10000; CHROMTEK 3H9-100 Lot#60706001AB) and then anti-rat-IgG-HRP secondary antibody (1:10000; SIGMA A9037-1ML Lot#SLCF6775). Ponceau staining was used as a loading control.
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bioRxiv - Biochemistry 2021Quote: ... the cells were washed with 1% BSA/1xPBS prior to addition of Pellets were incubated with 10 µl of mouse anti-rabbit IgG (Sigma RG-96; 1:100) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) were received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) was received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Developmental Biology 2022Quote: ... at 20°C overnight and then treated with the secondary antibody (AlexaFluor 488-conjugated goat anti-mouse IgG; Sigma-Aldrich; 1:1000 in PBS) at 20°C overnight ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168; anti-Rabbit IgG, Sigma, Catalog number A0545).
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... primary antibody against α-tubulin (T5168) and secondary antibodies (A0545, anti-rabbit; A9044, anti-mouse IgG-peroxidase) were purchased from Sigma-Aldrich (Darmstadt, Germany).
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed 10 min three times in PBS 1X-Tween buffer and then incubated for one h at room temperature with peroxydase labeled anti-rabbit donkey antibody (#NIF 824 ECL Rabbit IgG, HRP-linked whole Ab, Sigma-Aldrich Life Science, USA) diluted in saturation buffer at 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and then incubated with diluted sera and then plates were washed and incubated with HRP conjugated anti-mouse IgG (SIGMA, St. Louis, MO, USA). Results were read at 450 nm to collect end point ELISA data ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS 1X and incubated during 1h at room temperature with Alexa FluorTM 594 donkey anti-rabbit IgG (H+L) (Sigma-Aldrich, cat. nr A21207) secondary antibody 1:500 was used ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084, and Anti-Guinea Pig CF™ 647, 1:1000, Sigma Aldrich, SAB4600180) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... we used donkey anti-goat Igg H&L (Alexa Fluor® 647) Preadsorbed (Ab150135, 1:50) and sheep anti-rabbit Cy3 (Sigma–Aldrich C2306, 1:100).
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was subsequentially incubated with a horseradish peroxidase (HRP)-conjugated α-mouse whole IgG antibody produced in rabbit (Sigma-Aldrich, CAT. #A9044; 1:80000). Protein-HRP chemiluminescence was detected using Chemidoc XRS1 System (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 ng of F(ab’)2-Rabbit anti-Rabbit IgG (H+L) (CST, 4412S, Lane Danvers, MA, USA) was diluted in 0.1% Triton X-100 (Sigma-Aldrich, T8787, St. Louis, MO, USA) and incubated at 95°C for 5 min ...
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bioRxiv - Pathology 2024Quote: ... buffer the membranes were incubated for 1 hour at room temperature with anti-rabbit/mouse/goat IgG secondary antibody conjugated with horseradish peroxidase (1:5000; Sigma–Aldrich, St Louis, MO, USA). Signals was detected with Clarity enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2021Quote: ... 3h at 4°C under rotating with 10 µg of primary antibody (Supplementary Table S4; anti-mouse and anti-rabbit whole IgG (Millipore 12-371 and 12-370 respectively)) ...
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bioRxiv - Immunology 2020Quote: ... 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen, Karolinska Institute, Sweden) or 4 µg/IP anti-rabbit IgG control (Sigma-Aldrich Cat#I5006; RRID: AB_1163659). Two IPs were performed for LXR and pooled prior to DNA purification.
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... were subjected to immunoblot analysis with specific primary antibodies (Table 1) and secondary antibodies (anti-mouse/rabbit IgG coupled to horseradish peroxidase; Sigma Aldrich; 1:5000 dilution in blocking buffer). Detection of chemi-luminescent signal was performed with Clarity ECL Western Blotting substrate (BioRad ...