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Citations for Millipore Sigma :
1651 - 1700 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... anti-human FITC conjugated-IgG (AP113F;1:50 dilution) and anti-HA (H9658, clone HA-7;1:20,000 dilution) were from Sigma-Aldrich. Antibodies against SR-B1 for immunoblotting (ab217318 ...
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bioRxiv - Plant Biology 2021Quote: ... Bound-antibodies were located by linking to alkaline phosphatase-conjugated goat anti-rabbit IgG according to manufacturer’s instructions (Sigma).
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bioRxiv - Immunology 2019Quote: ... Horseradish peroxidase (HRP)-linked goat anti-rabbit and goat anti-mouse IgG secondary antibodies were purchased from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2019Quote: ... NP-40 was added to a final concentration of 0.15%(v/v) to the clarified extract which was then incubated with rabbit IgG (Sigma) coupled Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C at a 1:1000 at dilution and α-mouse IgG (whole molecule)-peroxidase (Sigma #9044) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... After incubation at room temperate for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... BL cells were spun down and plated at 0.5 × 10^6 cells in fresh media containing soluble anti-IgM or anti-IgG (Sigma) at concentrations and times indicated in figure legends (2.5-5 ug/mL for 22-48 hrs).
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bioRxiv - Cell Biology 2020Quote: ... The plates were then washed with PBS-T and an anti-IgG rabbit peroxidase (Sigma-Aldrich, St Louis, MO) was applied and incubated at room temperature for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl blocking buffer (10 mg/ml mouse IgG with 5 mM Azide) and 0.5 μl 0.5% Phenol red (Sigma P0290) were added for blocking the unconjugated Anti-Mouse-IgG-Atto647N and vortexed thoroughly ...
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bioRxiv - Microbiology 2022Quote: ... Immunized mice sera followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Antibody complexes were detected using the ImmobilonTM Western systems (Millipore).
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bioRxiv - Immunology 2020Quote: ... for 1 hour at RT and detection of anti-mouse IgG by the addition of pNPP substrate (Sigma-Aldrich). An arbitrary number of ELISA units were assigned to the reference pool and OD values of each dilution were fitted to a 4-parameter logistic curve using SOFTmax PRO software ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated sequentially with 500 ng/mL previously described mouse anti-ZIKV mAb ZV-165 and horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgG in PBS supplemented with 0.1% (w/v) saponin (Sigma) and 0.1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Immunology 2020Quote: ... were incubated overnight at 4°C with 50 μL of a solution of in 75 mM sodium carbonate (pH 9.6) containing either: 2.5 μg/ml aggregated human IgG (Sigma-Aldrich) for CP ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... Two additional 0.5 mL solubilisates of cerebellum and midbrain samples were incubated with 7.5 µg IgG (Millipore, 12-370), which were used as internal specificity control ...
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bioRxiv - Immunology 2022Quote: ... Secondary incubation was performed with 1:20 000 dilution of HRP-conjugated anti-human IgG antibody (Sigma Aldrich SAB701283) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... After incubation at room temperature for 1 hr with a 1:2,500 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Immunology 2022Quote: ... Fractions containing IgG were identified by SDS-PAGE under reducing/non-reducing conditions and concentrated using Centrifugal Filters (Millipore). Protein concentration was quantified with the BCA Assay Kit (Beyotime ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were incubated with 1.5 µg polyclonal anti-V5 or IgG for 1 h and captured with protein A agarose resin (P2545, Sigma) for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h in 20 mL TBS-T with 3% milk powder and 1:10,000 diluted goat anti-rabbit IgG-HRP (Sigma). Finally ...
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bioRxiv - Microbiology 2022Quote: ... the HBGA phenotype was detected with horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma-Aldrich, USA) for type A ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was immunoprecipitated with anti-PPARγ (A3409A, Perseus Proteomics, Tokyo, Japan) or anti-mouse IgG (Millipore, 12-371) antibody ...
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bioRxiv - Microbiology 2023Quote: ... we added a calibration range by serially diluting a polyclonal IgG antibody from human sera solution (reference: I2511, Sigma), to reach concentrations ranging from 0.68 ng.mL-1 to 22 ng.mL-1 ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SA1 and SA2 were immunoprecipitated with 10 µg SA1 and SA2 antibodies or non-specific IgG control (Sigma) conjugated to protein G dynabeads (Dynal ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Plant Biology 2023Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)– HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: ... or naïve polyclonal rabbit IgG (control for α-ORF2p: Innovative Research, #IRBIGGAP10MG; control for α-ZCCHC3: Millipore Sigma, #I5006). Total RNA controls were prepared by combining up to 35 µl of the clarified cell extracts with up to 500 µl of Trizol ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody incubation was performed for 30min with 1:10000 (v/v) anti-rabbit IgG-peroxidase antibody (Sigma-Aldrich). Again ...
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bioRxiv - Microbiology 2023Quote: ... gondii antibodies detection was conducted using an anti-monkey IgG FITC conjugate produced in rabbit (Sigma-Aldrich®, USA). T ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Tetraspanin and mouse IgG (mIgG) beads were washed in PBS with 0.1% (m/v) bovine serum albumin (BSA) (Sigma) prior to use ...
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bioRxiv - Microbiology 2023Quote: ... and FLAG-tagged proteins were detected by alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich, St. Louis, MO) and 1-Step NBT/BCIP substrate solution (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed in PBS-T (0.05% Tween-20 in PBS) and PBS before incubation with 1:10,000 dilution of HRP-conjugated anti-mouse IgG (Sigma) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... nonspecific antibody binding was blocked by incubation with an anti-CD16/32 antibody (clone 2.4G2; UCSF Ab production facility) and whole mouse IgG (Sigma) on ice for 15 min ...
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bioRxiv - Immunology 2024Quote: ... with the test mAb diluted to 5 µg/mL and AP-conjugated goat anti-mouse IgG (A3562 Sigma-Aldrich) used as a secondary.
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bioRxiv - Cell Biology 2024Quote: ... as the primary antibodies and Alexa Fluor 488 goat anti-rabbit IgG (Sigma-Aldrich, 11034, 1:1000 for IF), Alexa Fluor 488 goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The wells were incubated with 100 μl of 1/10,000 dilution of goat anti-rabbit-IgG alkaline phosphatase conjugate (Sigma) for 1 h at 20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881 ...
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bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-chicken IgY (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... an anti-GFP polyclonal antibody (Medical & Biological Laboratories) and a rabbit horseradish-peroxidase-linked IgG whole antibody (Sigma-Aldrich) were used as the primary and secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 8000 g for 10 min and pre-cleared by pre-incubated with 100 μl Mouse IgG−Agarose beads (A0919 Sigma) without antibody for 2 h at 4°C on wheel ...